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A preparation method of p(lla-cl)/collagen double-layer vascular stent coordinated by heparin and gemini factor

A collagen and vascular stent technology, applied in the directions of blood vessels, stents, anticoagulation treatment, etc., can solve the problems of unfavorable smooth muscle cell growth around the axis, unfavorable three-dimensional osmotic growth of smooth muscle cells, small nano/micro fiber pore size, etc. Promotes periaxial growth and three-dimensional migration, excellent mechanical properties and biocompatibility, and inhibits smooth muscle cell proliferation

Active Publication Date: 2018-04-20
DONGHUA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if dense and random nanofibers are used for the outer layer of vascular stents, there are many disadvantages: first, the nano / micro fibers obtained by traditional electrospinning have a small pore size, which is not conducive to the three-dimensional osmotic growth of smooth muscle cells; secondly, random Nanofibers are not conducive to inducing the growth of smooth muscle cells around the axis, and cannot achieve the effect of bionic natural vascular smooth muscle layer

Method used

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  • A preparation method of p(lla-cl)/collagen double-layer vascular stent coordinated by heparin and gemini factor
  • A preparation method of p(lla-cl)/collagen double-layer vascular stent coordinated by heparin and gemini factor
  • A preparation method of p(lla-cl)/collagen double-layer vascular stent coordinated by heparin and gemini factor

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Experimental program
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Embodiment 1

[0039] Weigh 0.9g of P(LLA-CL) and 0.3g of collagen with an electronic balance, dissolve them in 10mL of hexafluoroisopropanol, stir overnight until completely dissolved, and obtain P(LLA-CL) / collagen composite spinning solution; 0.3g heparin sodium and 20 μg VEGF were dissolved in 0.2mL diluent to obtain the drug solution loaded on the inner layer; 20 μg PDGF was dissolved in 0.3mL diluent to obtain the drug solution loaded on the outer layer, wherein the diluent was pH 7.2-7.4 PBS buffer solution and 0.1% bovine serum albumin BSA; the inner layer of the blood vessel is prepared by coaxial electrospinning, and the spinning parameters are as follows: the electrostatic high voltage is 16kV, and the shell layer solution (P(LLA-CL) / Collagen composite spinning liquid) propulsion speed is 1.0mL / h, core layer solution (inner layer loaded drug solution) propulsion speed is 0.1mL / h, receiving distance is 12cm, stainless steel rod rotation speed is 1000rpm; spinning is carried out 2 H...

Embodiment 2

[0041] Weigh 0.75g of P(LLA-CL) and 0.25g of collagen with an electronic balance, dissolve them in 10mL of hexafluoroisopropanol, stir overnight until completely dissolved, and obtain P(LLA-CL) / collagen composite spinning solution; 0.75g heparin sodium and 20 μg VEGF were dissolved in 0.5mL diluent to obtain the drug solution loaded on the inner layer; 20 μg PDGF was dissolved in 0.5mL diluent to obtain the drug solution loaded on the outer layer, wherein the diluent was pH 7.2-7.4 PBS buffer and 0.1% bovine serum albumin BSA; the inner layer of the tube is prepared by coaxial electrospinning, and the spinning parameters are as follows: the electrostatic high voltage is 14kV, the shell solution (P(LLA-CL) / collagen Protein composite spinning solution) advancing speed is 1.0mL / h, core solution (inner layer loaded drug solution) advancing speed is 0.1mL / h, receiving distance is 12cm, stainless steel rod rotation speed is 1200rpm; spinning is carried out for 5 hours Finally, the o...

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Abstract

The invention relates to a method for preparing a P(LLA-CL) / collagen double-layer vascular stent coordinated and regulated by heparin and gemini, comprising: mixing P(LLA-CL) and collagen in a solvent to obtain a composite spinning Silk liquid; dissolving heparin sodium and VEGF in the diluent to obtain the drug solution loaded on the inner layer; dissolving PDGF in the diluent to obtain the drug solution loaded on the outer layer; the drug solution loaded on the inner layer is the core layer, and the spinning solution is the shell The inner layer of the stent is obtained by coaxial electrospinning; the drug solution loaded on the outer layer is the core layer, and the spinning solution is the shell layer, and the outer layer of the stent is obtained by bidirectional conjugate electrospinning, which is continuously received in the inner layer of the stent On the outside, a double-layer vascular stent was obtained, cross-linked, and obtained. The vascular stent of the present invention has excellent mechanical properties and biocompatibility, can mimic the components, structures and functions of natural blood vessels, and is beneficial to the in situ regeneration of vascular tissues and the reconstruction of multilayer structures, and is of great importance in vascular tissue engineering. Applications.

Description

technical field [0001] The invention belongs to the field of preparation methods of double-layer vascular stents, and in particular relates to a preparation method of P(LLA-CL) / collagen double-layer vascular stents coordinated and regulated by heparin and geminifactors. Background technique [0002] The application of tissue engineered vascular stents should mainly meet the following requirements: First, vascular stents should have excellent biomechanical properties and vascular compliance, as well as excellent biocompatibility, to meet the support and histocompatibility of the vascular body structure Secondly, the lumen of the vascular stent should have long-term antithrombotic function, avoid the intimal hyperplasia of smooth muscle cells, accelerate endothelialization, and maintain the unobstructed blood flow; at the same time, the outer layer of the stent should promote the smooth muscle cell wrapping. Axial alignment and three-dimensional penetration can regulate the ph...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/26A61L27/24A61L27/18A61L27/54A61L27/58A61L33/10D04H1/728D04H1/76D04H1/4382D01D5/00A61F2/07
Inventor 莫秀梅吴桐王元非
Owner DONGHUA UNIV
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