Breeding method of temperature-resistant glutamic acid production strains

Abstract

The invention discloses a breeding method of temperature-resistant glutamic acid production strains. The method includes the steps of firstly, using the traditional strain breeding technology to perform mutagenesis on original strains, screening to obtain positive mutant strains with enhanced temperature resistance and increased glutamic acid production capacity; secondly; using lysozyme with the concentration of 1-2 g / L to perform enzymolysis on the strains for 3-5 hours to obtain glutamic acid production strain protoplasts; thirdly, irradiating with UV for 25-30 minutes, and heating to 55-60 DEG C for inactivation; fourthly, using polyethylene glycol for induced fusion; fifthly, performing progressive fusion on original strain multi-female parent protoplasts, and combining with multi-batch fermentation stability tests to breed the temperature-resistant glutamic acid production strains. The breeding method has the advantages that the method is high in temperature-resistant glutamic acid production strain screening efficiency; the temperature-resistant glutamic acid production strains bred by the method are high in acid production capacity, stable in genetic character and suitable for large-scale continuous production, continuous production and fermentation can be performed under high temperature, interference of high-temperature climate is overcome, and production cost can be controlled favorably.

Description

technical field [0001] The invention relates to a breeding method of a temperature-resistant glutamic acid-producing strain. Background technique [0002] Glutamic acid is the world's largest amino acid product. There are many factors affecting the fermentation level of glutamic acid, among which temperature is one of the main influencing factors. The most suitable temperature for the growth of traditional glutamic acid-producing bacteria is 30-34 °C. When the summer temperature is high, it is easy to affect the normal glutamic acid fermentation production. Especially in recent years, my country has maintained a high temperature in summer for a long time. It will cause the production enterprise to be forced to stop production. Therefore, the high temperature resistance of the glutamic acid producing strain is directly related to the production benefit. [0003] The temperature-resistant glutamic acid production strain can carry out continuous production and fermentation un...

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the defects that the existing glutamic acid strain breeding method is greatly affected by high temperature during strain fermentation, the production efficiency is low, and the genetic traits of the strain are unstable, and a kind of temperature-resistant grain production method is provided. Breeding method of acidic strain