Method for detecting pesticide residues through paper-based visualized molecular imprinting biosensor

Abstract

The invention provides a method for detecting pesticide residues through a paper-based visualized molecular imprinting biosensor, and discloses the visualized portable microfluidic paper chip sensor which is easy to operate and low in cost and is successfully applied to on-site detection. Through the sensor, a molecular imprinting technology is successfully introduced to a microfluidic paper chip, and color changing is triggered through a catalytic effect of zinc ferrite on hydrogen peroxide and 3,3',5,5'-tetramethyl benaidine to achieve visualized detection. The sensor is constructed through the following steps that hydrophobic patterns are printed in batches, wax melting forming is performed, and the zinc ferrite is prepared and synthesized; molecular imprinting polymers are synthesized in situ; a hydrogen peroxide solution is added to trigger a reaction, and the concentration range of the pesticide residues is determined by observing the color changes on the paper chip.

Description

technical field [0001] The present invention relates to the field of quantitative analysis and detection of pesticide residues, more specifically the preparation of a colorimetric biosensor for detecting pesticide residues based on molecular imprinting, and the invention also relates to the use of the paper-based molecular imprinting colorimetric sensor to detect pesticides in agricultural products residual method. Background technique [0002] Pesticide residue refers to the general term of pesticide parent, derivatives, metabolites, degradation products and impurities that remain in the environment, organisms and food after pesticide use. The main causes of excessive pesticide residues in vegetables are organophosphorus pesticides and carbamate pesticides that are prohibited from being used in vegetable production in some countries, such as methamidophos, omethoate, phorate, parathion, and methyl parathion wait. Eating food containing a large amount of highly toxic and h...

AI Technical Summary

Problems solved by technology

Gas chromatography-mass spectrometry is the most widely used method for pesticide residue analysis. Gas chromatography-mass spectrometry is used for pesticide residue analysis. However, traditional gas chromatography-mass spectrometry and other pesticide residue analysis techniques are expensive and time-consuming, which poses a challenge to the food safety supervision department. The pre-production, mid-production and post-production supervision of agricultural products has brought a lot of inconvenience, so it has also spawned a large number of rapid detection technologies for pesticide residues. The common ones are chemical rapid detection method, immunoassay method, enzyme inhibition method and living body detection method. Wait

However, the above-mentioned methods are expensive, complicated to operate, and have a strong dependence on the external environment, which is not conducive to on-site detection anytime and anywhere, which limits its current application and development.

Images