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Culture media for tissue culture of red leaf pistacia chinensis, application and culture method

A technology of tissue culture and culture method, which is applied in the field of culture medium for producing red-leaf Pistacia chinensis seedlings, can solve the problems that the germination rate and survival rate are not very high, and cannot meet the needs of seedlings, so as to maintain genetic stability and improve germination rate, increase the effect of reproduction coefficient

Inactive Publication Date: 2015-12-23
SICHUAN COLORLINK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, Pistacia chinensis is mainly reproduced by sowing and grafting, but the germination rate and survival rate are not very high, which is far from meeting the domestic demand for seedlings

Method used

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  • Culture media for tissue culture of red leaf pistacia chinensis, application and culture method
  • Culture media for tissue culture of red leaf pistacia chinensis, application and culture method
  • Culture media for tissue culture of red leaf pistacia chinensis, application and culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) healthy, aseptic axillary buds that germinate are cut into 2cm band bud stem segments, as spare materials;

[0038] (2) Carry out the primary culture of the test material according to the sequence of the aforementioned steps to obtain differentiated buds, and inoculate the gained buds on MS proliferation medium with different concentrations of hormones in turn, inoculate 20 bottles for each concentration gradient, and set 3 repetitions, Each culture bottle is connected with 3 stem sections with buds, and the multiplication coefficient is counted;

[0039] (3) Place the inoculated test material under the culture conditions of light 2500Lx, temperature 25±2°C, light time 12h / d, and carry out routine culture;

[0040] (4) Every 7 days, the proliferation situation of buds is counted once, and the test period is 35 days;

[0041] (5) Process and analyze the test data.

[0042] Proliferation treatment comparison test A:

[0043]

[0044] Proliferation treatment comp...

Embodiment 2

[0049] (1) the clustered shoots gained from the multiplication culture are separated into individual plants, as spare materials;

[0050] (2) test material is inoculated on the 1 / 2MS rooting medium that adds different concentrations of hormones, each concentration gradient inoculates 20 bottles, establishes 3 repetitions, and each culture bottle is connected with 3 clustered buds, and the rooting rate is counted;

[0051] (3) Place the inoculated test material under the culture conditions of light 2500Lx, temperature 25±2°C, light time 12h / d, and carry out routine culture;

[0052] (4) Every 7 days, the rooting situation is counted once, and the test period is 35 days;

[0053] (5) Process and analyze the test data.

[0054] Rooting treatment comparative test A

[0055]

[0056] Rooting treatment comparison test B

[0057]

[0058]

[0059] From the comparison of the test data table, it can be seen that the rooting rate is the highest on the medium of test design 3, ...

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Abstract

The invention discloses culture media, application and a culture method and particularly relates to culture media for tissue culture of red leaf pistacia chinensis, application and a culture method. The culture media are divided into the primary generation culture medium, the enrichment culture medium and the rooting culture medium. The enrichment culture medium is prepared from MS, 1.5-2.5 mg / L of 6-benzyladenine, 0.05-0.15 mg / L of naphthylacetic acid, 15-25 g / L of saccharose and 5.5-6.5 g / L of agar. The rooting culture medium is prepared from 1 / 2MS, 0.15-0.25 mg / L of naphthylacetic acid, 0.75-0.85 mg / L of 3-indolebutyric acid, 15-25 g / L of saccharose, 5.5-6.5 g / L of agar amd 0.15-0.25 mg / L of active carbon. The pH of the enrichment culture medium is regulated to be 5.5-6.5 before sterilization is conducted, and the pH of the rooting culture medium is regulated to be 5.5-6.0 before sterilization is conducted. The culture media can effectively improve the sprouting ratio and the survival rate of the red leaf pistacia chinensis, and effectively increase the reproduction coefficient of the red leaf pistacia chinensis while the genetic stability of fine tree species is maintained.

Description

technical field [0001] The invention relates to a culture medium, its application and a cultivation method, in particular to the culture medium, its application and its cultivation method for producing red leaf pistachio chinensis seedlings by tissue culture technology. Background technique [0002] Red-leaved Pistacia chinensis, also known as Kaishu, Kaimu, Huanglian, etc., belongs to the deciduous tree of Pistacia genus in the Anacardaceae family. The adult tree is more than 20m high and has excellent material. The leaves show different colors with the seasons. Afforestation, is a multipurpose tree species. Because its seeds have an oil content of 42.5% and an oil yield of 20% to 30%, it can be refined into biodiesel. In recent years, Pistacia chinensis has been listed by the country as one of the main biomass energy tree species that will be developed during the "Eleventh Five-Year Plan" period, and has great development potential. [0003] At present, Pistacia chinensi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李刚余金莲周棚
Owner SICHUAN COLORLINK CO LTD
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