Multi-mode microimaging method based on programmable LED array illumination

Abstract

The invention discloses a multi-mode microimaging method based on programmable LED array illumination. An LED array used as an illumination light source of a microimaging system is directly installed below a sample carrying bench of the microimaging system and the center of the LED array is located at an optical axis of the microimaging system, thereby realizing phase contrast, optical field imaging, and optical dyeing imaging modes. With the method, dyeing microimaging can be realized flexibly and no additional optical element like an annular diaphragm, a microlens array, and a phase plate and the like needs to be added in an imaging light path of a microscopic, so that the system structure is simplified and the cost is substantially reduced.

Description

technical field [0001] The invention belongs to optical microscopic imaging technology, in particular to a multi-mode microscopic imaging method based on programmable LED array illumination. Background technique [0002] Phase contrast imaging and polarized light imaging are two common microscopic imaging methods, and the corresponding microscopes are called phase contrast microscope and polarized light microscope respectively. Phase contrast microscope, also known as phase contrast microscope, was invented by Dutch scientist Zernike in 1935 (Sheng Fugen. Principle Analysis of Differential Interference Phase Contrast Microscope (Vector Method) [J]. Optical Instruments, 1986, (5).), Microscope for viewing unstained specimens. Phase-contrast microscopy uses the difference in refractive index and thickness between different structural components of the object to convert the optical path difference passing through different parts of the object into the difference in amplitude (...

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Problems solved by technology

[0005] In order to solve this problem, the invention patent "Multi-mode Microscopic Imaging System Based on LED Array and Its Method" (Application No.: 201510186306.5) uses LED array as the microscope illumination source, and realizes bright field, Three microscopic imaging modes of dark field and differential phase contrast solve the problems of complex optical path and difficult operation of traditional microscopes in bright field, dark field, and differential phase contrast imaging

However, there are still many problems that cannot be solved in these existing technologies on how to use LED arrays to realize phase contrast imaging, light field imaging and optical dyeing microscopy imaging.

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