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Method for extracting plasma protein powder from pig blood

A technology of plasma protein powder and pig blood, which is applied in the field of separation and extraction of biologically active substances, can solve the problems of long time consumption, high energy consumption of the process, and the production of a single product, so as to reduce production costs, overcome the problems of single product, and easy absorption and utilization Effect

Inactive Publication Date: 2015-12-09
HAIAN HUARUN FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

From the direct consumption of pig blood tofu to the production of enzymatic pig blood meal and compound amino acid powder, great progress has been made, but it still cannot meet the needs of human beings.
The problem is that most of the processes are not perfect. For example, some processes consume a lot of energy and take a long time, some processes can only produce a single product, some processes have low yield, some processes use a large amount of organic solvents, and there are environmental protection defects, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] In this embodiment, the composite protease is formed by mixing trypsin, alkaline protease, papain and bromelain in a certain proportion. The specific steps are:

[0021] 1) Preparation of anticoagulant pig blood: fresh pig blood qualified for quarantine is put into the first container, which has been added with 1.0% sodium citrate, 1.0% disodium edetate, 1.0% ethyl alcohol An anticoagulant composed of dipotassium diaminetetraacetic acid, 0.6% sodium hexametaphosphate and 0.8% sodium pyrophosphate, fully stirred evenly, stored in a tank at 7°C for 42 hours;

[0022] 2) Separation of plasma and blood cells: After storage at 7°C for 42 hours, the upper layer of anticoagulated pig blood is a colorless transparent liquid called plasma, and the lower layer of dark red viscous liquid is blood cells. The upper layer of plasma is sucked into the second container by siphon method Middle; after the remaining blood cells were centrifuged at a speed of 2500r / min, the upper layer of...

Embodiment 2

[0029] In this embodiment, the composite protease is formed by mixing trypsin, alkaline protease, papain and bromelain in a certain proportion. The specific steps are:

[0030] 1) Preparation of anticoagulant pig blood: fresh pig blood qualified for quarantine is put into the first container, which has been added with 1.0% sodium citrate, 1.0% disodium edetate, 1.0% ethyl alcohol An anticoagulant composed of dipotassium diaminetetraacetic acid, 0.6% sodium hexametaphosphate and 0.8% sodium pyrophosphate, fully stirred evenly, stored in a tank at 7°C for 42 hours;

[0031] 2) Separation of plasma and blood cells: After storage at 7°C for 42 hours, the upper layer of anticoagulated pig blood is a colorless transparent liquid called plasma, and the lower layer of dark red viscous liquid is blood cells. The upper layer of plasma is sucked into the second container by siphon method Middle; after the remaining blood cells were centrifuged at a speed of 2500r / min, the upper layer of...

Embodiment 3

[0039] In this embodiment, the composite protease is formed by mixing trypsin, alkaline protease, papain and bromelain in a certain proportion. The specific steps are:

[0040]1) Preparation of anticoagulant pig blood: fresh pig blood qualified for quarantine is put into the first container, which has been added with 1.0% sodium citrate, 1.0% disodium edetate, 1.0% ethyl alcohol An anticoagulant composed of dipotassium diaminetetraacetic acid, 0.6% sodium hexametaphosphate and 0.8% sodium pyrophosphate, fully stirred evenly, stored in a tank at 8°C for 42 hours;

[0041] 2) Separation of plasma and blood cells: After storage at 8°C for 42 hours, the upper layer of anticoagulated pig blood is a colorless transparent liquid called plasma, and the lower layer of dark red viscous liquid is blood cells. The upper layer of plasma is sucked into the second container by siphon method Middle; after the remaining blood cells were centrifuged at a speed of 2500r / min, the upper layer of ...

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Abstract

The invention provides a method for extracting plasma protein powder from pig blood. The method comprises the following steps of preparing anticoagulation pig blood; separating plasma from haemocyte; depositing the plasma to collect prothrombin; preparing crude thrombin liquid; preparing fine thrombin products; breaking walls and performing hemolysis of the haemocyte; performing enzymolysis of hyperglobulinemia by segment; preparing hydrolyzed protein of the pig blood. The method has the beneficial effects that the amino acid content of the hydrolyzed protein of the pig blood is more than 90% of protein, the hydrolyzed protein of the pig blood is easy to absorb and utilize, raw materials are fully utilized, and two high added-value products can be produced by once charging. In the whole process of production, organic solvents with large toxicity are not used, the defects of product singleness, solvent residue and the like are overcome, and the production cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of separation and extraction of bioactive substances, in particular to a method for extracting plasma protein powder from pig blood. Background technique [0002] Pig blood is a high-quality protein, and nutritionists call it liquid meat. Pig blood contains almost all the nutrients needed by the human body: protein, amino acids, vitamins, sugar and sodium, potassium, iron, calcium and other indispensable trace elements for the human body. Among them, 18.9% protein, 1.2% non-protein organisms, 0.9% minerals, and 79% water. In addition, its amino acid composition is balanced, and the total amount of 8 essential amino acids required by the human body is higher than that of human milk and whole eggs, especially lysine The amino acid content is as high as 9%, which is in line with the recommended mode of the United Nations Food and Agriculture Organization and the World Health Organization (FAO / WHO) expert group...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/74C07K14/47C12P21/06
CPCC12N9/6429C07K14/4717C12P21/06C12Y304/21005
Inventor 许映文
Owner HAIAN HUARUN FOOD
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