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Method of removing pollutant from cadmium-containing sewage

A technology for pollutants and sewage, applied in the fields of water pollutants, chemical instruments and methods, water/sewage multi-stage treatment, etc., can solve problems such as ecological environment pollution, human physical and mental health hazards, and complexity, and achieve good application prospects.

Inactive Publication Date: 2015-12-09
河南省地质环境规划设计院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The composition of industrial sewage is relatively complex, especially a large number of artificially synthesized compounds entering the environment. Such substances are mainly ammonia nitrogen, sulfide and phosphorus-containing compounds. Due to the complexity of the structure of these substances, they cannot be decomposed and utilized by microorganisms in a short time. The traditional wastewater treatment method using activated sludge to cultivate and domesticate microorganisms can no longer effectively remove these pollutants. These substances have accumulated in the environment for a long time, causing great pollution to the ecological environment on which we live, and affecting human physical and mental health. bring great harm
A considerable number of industrial polluting enterprises in our country would rather be punished than invest in wastewater treatment. Even if there is a sewage treatment plant, the operation is extremely abnormal.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for removing pollutants in sewage, comprising the steps of:

[0027] Preparation of composite microbial agent: mix the mixed bacterial liquid and the carrier according to the weight ratio of 1:1, stir evenly, then leave it to stand for 6 hours, and finally put it in 4°C for low-temperature drying, and control the water content at 6% after drying, to obtain final product The carrier is obtained by mixing bamboo charcoal, chitosan and diatomaceous earth according to the mass ratio of 2:2:1; the above-mentioned mixed bacteria liquid is mixed by the raw material bacteria of the following parts by weight: 10 parts of Rhodococcus, 9 parts of Thiobacillus denitrificans , 7 parts of Pseudomonas stutzeri, 6 parts of Sphingomonas, 5 parts of Bacillus pumilus, 2 parts of Phanerochaete chrysosporium; the concentrations of the above-mentioned raw material bacteria were all controlled at 1×10 8 pieces / ml.

[0028] Sewage pretreatment: First, the sewage (NH3-N is 300mg / L, su...

Embodiment 2

[0031] Use DNAMAN software to design primers, add BamHI and SalI restriction sites respectively, synthesize the nucleotide sequence HP gene according to the amino acid sequence NP_744955.1 whole gene, obtain the target fragment by amplification, and obtain the target gene by PCR amplification HP (the corresponding mutation sites 71S / T, 89T / Y, 121T / A, 143D / S, 184S / Q, 239W / G, 246V / P, 269K / N, 304P / S, 353K / E, 369G / D, 372C / S, 422D / R, and 448G / I were introduced into the gene sequence, thus obtaining different mutant genes), the PCR product was double-digested with BamHI and SalI, and the PCR product was double digested with BamHI and SalI The digested cloning expression vector PWB980 was connected, and the successfully verified recombinant plasmid was transformed into the Phanerochaetechrysosporium (Phanerochaetechrysosporium) ATCC24725 to obtain a cadmium-degrading genetically engineered bacterium.

Embodiment 3

[0032] Example 3 Verification of the sewage treatment effect of Phanerochaete chrysosporium genetically engineered bacteria and other bacterial agents

[0033]According to the method of Example 1, the corresponding sewage treatment experiment was carried out, and the sewage and the sewage of Example 1 belonged to the same batch and had the same concentration of pollutants. Wherein each component composition of bacterial agent is identical with embodiment 1.

[0034] The genetically engineered bacteria of different mutation sites obtained in Example 2 were used to carry out the reaction of removing cadmium, and it was found through experiments that 71S / T, 89T / Y, 121T / A, 143D / S, 184S / Q, 239W / G, 246V / P, 269K / N, 304P / S, 353K / E, 369G / D, 372C / S, 422D / R, 448G / I mutants all have significantly enhanced effects compared with the original strain. The total treatment time is not more than 4 days, and the result is as follows: the sewage is the same batch of sewage, so as to ensure the sa...

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Abstract

The invention relates to a method of removing pollutants from cadmium-containing sewage, wherein the method includes the steps of: (a) enabling the sewage to pass through a solid-liquid separator and performing solid-liquid separation to remove large solid granular substances; (b) enabling the sewage to enter into a sedimentation tank, performing sedimentation for 12 h, and enabling the sewage to pass through a circular-mesh filter screen to remove solid flocculates; (c) feeding the filtered sewage into a biological reaction tank, regulating the pH to 7, adding a composite microbial inoculant to the sewage by 10 g per cubic meter of the sewage by one time per day, wherein the microbial inoculants is added continuously for one week; and (d) allowing the sewage for stand for 3 days and discharging the water. The method can effectively remove pollutants containing ammonia nitrogen, sulfur and phosphorus and cadmium, is low in investment cost and has excellent application prospect.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a method for removing pollutants in sewage. Background technique [0002] With the development of industrial production and the improvement of people's living standards, the volume of industrial sewage and urban domestic sewage is increasing at an alarming rate. One of the reasons for threatening the human living environment. [0003] In order to meet the continuous improvement of the public's requirements for environmental quality, the country has formulated more and more stringent emission standards for nitrogen. Research and development of economical and efficient nitrogen removal treatment technology has become the focus and focus of water pollution control engineering research. Although there are many methods that can effectively remove ammonia, such as physical methods include reverse osmosis, distillation, and soil irrigation; chemical methods...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F9/14C02F101/22C02F101/20
CPCC02F1/001C02F1/52C02F1/66C02F3/34C02F3/348C02F9/00C02F2001/007C02F2101/101C02F2101/105C02F2101/16C02F2101/20C02F2209/06C02F2209/10C02F2301/08C02F2303/24C07K14/00C07K14/21C12N1/14C12N1/20
Inventor 李娜
Owner 河南省地质环境规划设计院有限公司
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