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Detection reagent box for sulfydryl compounds in urine and preparation method and application

A technology for detection kits and detection reagents, which is applied in the direction of analyzing materials through chemical reactions and observing the impact on chemical indicators. and low accuracy, to achieve low false positive rate and false negative rate and improve accuracy

Active Publication Date: 2015-12-02
无锡先君生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a detection kit for sulfhydryl compounds in urine, its preparation method and application, which are used to solve the problems of prominent false negative and misjudgment of current sulfhydryl detection reagents and low detection accuracy and precision

Method used

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  • Detection reagent box for sulfydryl compounds in urine and preparation method and application

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Embodiment 1

[0030] To make 1000 thiol compounds detection kits in urine, the process is as follows:

[0031] 1. Preparation of detection reagent: Dissolve mercurous chloride and trichloroacetic acid in distilled water respectively, configure the concentration of mercurous chloride to be 0.001mol / L, and the mass concentration of trichloroacetic acid to be 10wt%, and separate them in a sterile environment. Fill into 5ml ampoules and wire drawing potting, each ampule contains 1ml, and prepare 1000 detection reagents accordingly;

[0032] 2. Preparation of contrast reagent: dissolve mercuric chloride, mercurous chloride and trichloroacetic acid in distilled water, configure the concentration of mercuric chloride to be 0.04mol / L, the concentration of mercurous chloride to be 0.001mol / L, trichloro The mass concentration of acetic acid is 10wt%, and it is divided into 5ml ampoules in a sterile environment and drawn and sealed. The filling capacity of each ampoule is 1ml, and 1000 control reagent...

Embodiment 2

[0040] To make 1000 thiol compounds detection kits in urine, the process is as follows:

[0041] 1. Preparation of detection reagent: Dissolve mercurous chloride and trichloroacetic acid in distilled water respectively, configure the concentration of mercurous chloride to be 0.001mol / L, and the mass concentration of trichloroacetic acid to be 10wt%, and separate them in a sterile environment. Fill into 5ml ampoules and wire drawing potting, each ampule contains 1ml, and prepare 1000 detection reagents accordingly;

[0042] 2. Preparation of contrast reagent: dissolve mercuric chloride, mercurous chloride and trichloroacetic acid in distilled water, configure the concentration of mercuric chloride to be 0.045mol / L, the concentration of mercurous chloride to be 0.001mol / L, trichloro The mass concentration of acetic acid is 10wt%, and it is divided into 5ml ampoules under aseptic environment and drawn and sealed, and the filling capacity of each ampoule is 1ml, and 1000 contrast re...

Embodiment 3

[0050] To make 1000 thiol compounds detection kits in urine, the process is as follows:

[0051] 1. Preparation of detection reagents: Dissolve mercurous chloride and trichloroacetic acid in distilled water respectively, configure the concentration of mercurous chloride to 0.001mol / L, and the mass concentration of trichloroacetic acid to 10%, and separate them in a sterile environment. Fill into 5ml ampoules and wire drawing potting, each ampule contains 1ml, and prepare 1000 detection reagents accordingly;

[0052] 2. Preparation of contrast reagent: dissolve mercuric chloride, mercurous chloride and trichloroacetic acid in distilled water, configure the concentration of mercuric chloride to be 0.04mol / L, the concentration of mercurous chloride to be 0.001mol / L, trichloro The mass concentration of acetic acid is 10%, and it is divided into 5ml ampoules in a sterile environment and drawn and sealed.

[0053] 3. Preparation of color reagent: the concentration of phosphotungsti...

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Abstract

The invention relates to a urine detection reagent in the medical examination field, in particular to a detection reagent box for sulfydryl compounds in urine and a preparation method and application. The detection reagent box for sulfydryl compounds in urine comprises a detection reagent, a contrast reagent and a color developing reagent, wherein the detection reagent is an aqueous solution containing a mercurous ionic compound and trichloroacetic acid, the contrast reagent is an aqueous solution containing a mercury ionic compound, a mercurous ionic compound and trichloroacetic acid, and the color developing reagent is a buffering solution containing phosphotungstic acid, silicotungstic acid and a lithium ion compound. The detection reagent box for sulfydryl compounds in urine can be used for screening and auxiliary diagnosis of cervical cancer. Compared with the prior art, the technical scheme is simple in technology and high in stability, the false positive rate and the false negative rate are low according to the detection result, and the detection reagent box for sulfydryl compounds in urine is suitable for screening and auxiliary diagnosis of cervical cancer.

Description

technical field [0001] The invention relates to a urine detection reagent in the field of medical testing, in particular to a detection kit for sulfhydryl compounds in urine, a preparation method and application thereof. Background technique [0002] Among the malignant tumors that endanger women's health in the world, cervical cancer is one of the most common gynecological malignant tumors in women worldwide after breast cancer. Its incidence rate ranks first in some developing countries. According to estimates by the International Cancer Research Center, there are approximately 371,200 new cases of cervical cancer each year, accounting for 9.8% of all tumors, accounting for 15% of new cases in women, of which 78% are Cases occurred in developing countries, only 4.4% in developed countries. my country has a vast territory and a large population. According to the large-scale national population death cause survey organized by the National Cancer Prevention and Research Offi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 刘东郑明彬黎磊
Owner 无锡先君生物医药有限公司
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