A kind of shiitake mushroom liquid fermentation medium and the method for producing shiitake mushroom
A fermenter, liquid strain technology, applied in mushroom cultivation, botanical equipment and methods, cultivation and other directions, can solve the problems of high cost, long mycelium cultivation cycle, low mushroom efficiency, etc., and achieves low price, easy source, The effect of short production cycle
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Embodiment 1
[0041] According to the formula of bran, weigh it according to a 500L standard fermentation tank: 10000g of bran, 5000g of soybean meal, 3000g of glucose, 500g of potassium dihydrogen phosphate, 250g of magnesium sulfate, 100g of CMC-Na (cellulase inducer), and put them into a 200L stainless steel frying pan. In a cooking pot, add 100L of water and stir to dissolve, boil for 20 minutes and then filter, add water to the filtrate to 500L, and add 350g of fresh soybean oil, set aside. The fermenter was emptied for 2 hours under the conditions of steam pressure 0.13-0.15Mpa and temperature 121°C; the above-mentioned prepared liquid fermenter culture medium was filled into tanks, and it was sterilized under the conditions of steam pressure 0.13-0.15Mpa and temperature 121°C for 40 minutes. After the internal temperature of the liquid in the fermenter is cooled to below 28° C., inoculate under sterile conditions with an inoculum size of 2% (v / v). Fermentation culture temperature is ...
Embodiment 2
[0043] According to the formula of bran, weigh it according to a 500L standard fermentation tank: 10000g of bran, 5000g of soybean meal, 3000g of glucose, 500g of potassium dihydrogen phosphate, 250g of magnesium sulfate, 100g of CMC-Na (cellulase inducer), and put them into a 200L stainless steel frying pan. In a cooking pot, add 100L of water and stir to dissolve, boil for 20 minutes and then filter, add water to the filtrate to 500L, and add 350g of fresh soybean oil, set aside. The fermenter was emptied for 2 hours under the conditions of steam pressure 0.13-0.15Mpa and temperature 121°C; the above-mentioned prepared liquid fermenter culture medium was filled into tanks, and it was sterilized under the conditions of steam pressure 0.13-0.15Mpa and temperature 121°C for 40 minutes. After the internal temperature of the liquid in the fermenter is cooled to below 28°C, inoculate under aseptic conditions, and inject 1000 mL of liquid parent seed of shiitake mushrooms into a bot...
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