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Application of chlorogenic acid serving as inhibitor of Sortase A

A chlorogenic acid and inhibitor technology, applied in the field of chlorogenic acid, can solve problems such as application that have not been reported

Inactive Publication Date: 2015-12-02
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chlorogenic acid is widely used, mainly in pharmaceutical, daily chemical and food industries, and there is no report on its application in SortaseA inhibitors

Method used

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  • Application of chlorogenic acid serving as inhibitor of Sortase A
  • Application of chlorogenic acid serving as inhibitor of Sortase A
  • Application of chlorogenic acid serving as inhibitor of Sortase A

Examples

Experimental program
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Embodiment

[0019] The strains and plasmids used in this example are listed in Table 1. The fluorescent peptide DABCYL-QALPETGEE-EDANS used was synthesized by Jill Biochemical. Compound chlorogenic acid (CHA) was purchased from the National Food and Drug Inspection Institute.

[0020] Table 1 Bacteria and plasmid list

[0021]

[0022]

[0023] Escherichia coli strains were inoculated in LB medium, cultured with shaking at 37°C, and ampicillin (100 μg / mL) was added. Staphylococcus aureus strains were inoculated in brain heart infusion (BHI) broth (Sigma Company), cultured with vigorous shaking at 37°C, and erythromycin (EM, 2.5 μg / mL) and chloramphenicol (15 μg / mL) were added. . Recombinant SrtA of S. aureus was purified from E. coli strain BL21(DE3) by GST affinity chromatography. The designated PsrtA59F and PsrtA59R primers were selected from Staphylococcus aureus Newman genomic DNA to amplify the SrtAΔN59 sequence (only expressing 60-206), and cloned into the pGEX-6P-1 vector...

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Abstract

The invention discloses new application of chlorogenic acid, particularly relates to application of chlorogenic acid serving as an inhibitor of Sortase A, and the application is characterized in that chlorogenic acid can be directly added and plant extracts taking chlorogenic acid as main components can serve as an additive for treatment of pathogenic bacterial infection. The application of chlorogenic acid serving as the inhibitor of Sortase A, provided by the invention, has the advantages as follows: the activity of Sortase A can be effectively inhibited under the condition that the addition dosage of chlorogenic acid is much lower than the MIC (minimum inhibitory concentration) thereof, therefore the adhesion between Sortase A and extracellular matrix can be inhibited, the infection caused by pathogenic bacteria like staphylococcus aureus can be effectively treated; besides, the action mechanism taking virulence factors as the target is different from that of conventional antibiotics, the drug resistance is not easily produced, the cross tolerance to conventional antibiotics is avoided, and clinical infection caused by drug resistant pathogenic bacteria also can be effectively treated.

Description

technical field [0001] The invention relates to the application of chlorogenic acid, in particular to the application of chlorogenic acid as a SortaseA inhibitor. Background technique [0002] Chlorogenic acid is a depsipphenolic acid composed of caffeic acid and quinic acid. It has the same name as coffee tannic acid, chemical name 3-0-caffeoylquinic acid, molecular formula: C16H1809, molecular weight: 354.30. A phenylpropanoid produced by the shikimate pathway during respiration. Chlorogenic acid is an important biologically active substance, which has the functions of antibacterial, antiviral, increasing white blood cells, protecting liver and gallbladder, antitumor, lowering blood pressure, lowering blood fat, scavenging free radicals and stimulating the central nervous system. It is one of the active ingredients of many traditional Chinese medicinal materials, and it is also an index ingredient of some patented medicines. SortaseA is a key protease that mediates the a...

Claims

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Application Information

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IPC IPC(8): A61K31/216A61P31/04
Inventor 邓旭明王大成王琳向华王铁东
Owner JILIN UNIV
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