Health-care soy sauce rich in soybean polysaccharide and preparation method thereof
A technology for health-care soy sauce and soybean polysaccharide, which is applied in the directions of polysaccharide/gum-containing food ingredients, food science, etc., can solve the problems of high cost and complicated preparation process of health-care soy sauce, and achieve the effect of facilitating natural color, rich nutrition and improving total nitrogen.
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Embodiment 1
[0036] (a) Strain activation: Prepare PDA medium (potato dextrose agar medium) according to the conventional method. Specifically, weigh 200g of peeled potatoes and cut them into small pieces, add water and boil them (boil for 20 to 30 minutes, and can be soaked by glass rods). Just puncture), filter with eight layers of gauze, heat, add 15 g of agar powder, continue to heat and mix well, after the agar is dissolved, add 20 g of glucose, stir evenly, and then make up water to 1000 mL after cooling down slightly. Pack test tubes, stopper, bandage, sterilize at 121°C for 30 min, take out the test tubes and put them on a slant, cool down, inoculate Aspergillus oryzae strain 3042 and Trichoderma viride respectively on the PDA slant medium in a sterile state, to make Aspergillus oryzae Cultivate at a constant temperature of 32°C for 72 hours; Trichoderma viride is cultivated at a constant temperature of 28°C for 48 hours, and the slopes are all covered with spores;
[0037] (b) Pre...
Embodiment 2
[0042] (a) Strain activation: Prepare PDA medium (potato dextrose agar medium) according to the conventional method. Specifically, weigh 200g of peeled potatoes and cut them into small pieces, add water and boil them (boil for 20 to 30 minutes, and can be soaked by glass rods). Just puncture), filter with eight layers of gauze, heat, add 15 g of agar powder, continue to heat and mix well, after the agar is dissolved, add 20 g of glucose, stir evenly, and then make up water to 1000 mL after cooling down slightly. Pack test tubes, stopper, bandage, sterilize at 121°C for 30 min, take out the test tubes and put them on a slant, cool down, inoculate Aspergillus oryzae strain 3042 and Trichoderma viride respectively on the PDA slant medium in a sterile state, to make Aspergillus oryzae Cultivate at a constant temperature of 32°C for 72 hours; Trichoderma viride is cultivated at a constant temperature of 28°C for 48 hours, and the slopes are all covered with spores;
[0043] (b) Pre...
Embodiment 3
[0048] (a) Strain activation: Prepare PDA medium (potato dextrose agar medium) according to the conventional method. Specifically, weigh 200g of peeled potatoes and cut them into small pieces, add water and boil them (boil for 20 to 30 minutes, and can be soaked by glass rods). Just puncture), filter with eight layers of gauze, heat, add 15 g of agar powder, continue to heat and mix well, after the agar is dissolved, add 20 g of glucose, stir evenly, and then make up water to 1000 mL after cooling down slightly. Pack test tubes, stopper and bandage, sterilize at 121°C for 30 min, take out the test tubes and put them on a slope, cool them down, and inoculate Aspergillus sojae and Trichoderma viride on the PDA slope medium under aseptic conditions, so that Aspergillus oryzae can grow at a temperature of Cultivate at a constant temperature of 32°C for 72 hours; Trichoderma viride is cultivated at a constant temperature of 28°C for 48 hours, and the slopes are all covered with spor...
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