Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Modified diatomite carried immobilized spore laccase as well as preparation method and application thereof

A technology of spore laccase and diatomite, which is applied to chemical instruments and methods, immobilized on or in inorganic carriers, water pollutants, etc., can solve problems such as inability to immobilize spores, and achieves low production cost and abundant resources. , the effect of simple process

Active Publication Date: 2015-11-18
JIANGNAN UNIV
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the experience of traditional immobilized enzymes and immobilized cells cannot be fully applied to the immobilization of spores

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Modified diatomite carried immobilized spore laccase as well as preparation method and application thereof
  • Modified diatomite carried immobilized spore laccase as well as preparation method and application thereof
  • Modified diatomite carried immobilized spore laccase as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1 diatomaceous earth amination and spore laccase immobilization method 1

[0048] Weigh 2g of diatomaceous earth, pour it into 95ml of absolute ethanol, add 5ml of 3-aminopropyltriethoxysilane at the same time, shake the mixture, and treat it with ultrasonic wave for 30min. The above-treated mixture was treated at 70°C and 200r / min for 5h. The modified diatomite treated above was repeatedly washed and collected by filtration.

[0049] Dip the diatomite prepared above into 40ml potassium dihydrogen phosphate-dipotassium hydrogen phosphate buffer solution (pH7.0, 50mM), add 5ml carbodiimide hydrochloride (2mg / ml) and sonicate for 10min, then add 3ml of spore suspension (100mg / ml, wet weight) was ultrasonically treated for 30min, transferred to a shaker at 200r / min, and fixed at 37°C for 12h. Wash 3 times with buffer and collect by filtration. The recovery rate of enzyme activity was measured to be 82.29%.

Embodiment 2

[0050] Embodiment 2 diatomite amination and spore laccase immobilization method 2

[0051] Weigh 2g of diatomaceous earth, pour it into 48ml of absolute ethanol, add 2ml of 3-aminopropyltriethoxysilane at the same time, shake the mixture, and treat it with ultrasonic wave for 30min. The above-treated mixture was treated at 70°C and 200r / min for 12h. The modified diatomite treated above was repeatedly washed and collected by filtration.

[0052] Dip the diatomite prepared above into 40ml of potassium dihydrogen phosphate-dipotassium hydrogen phosphate buffer solution (pH7.0, 50mM), add 4ml of carbodiimide hydrochloride (4mg / ml) and sonicate for 10min, then add 2ml of spore suspension (100mg / ml, wet weight) was ultrasonically treated for 30min, transferred to a shaker at 200r / min, and fixed at 37°C for 12h. Wash 3 times with buffer and collect by filtration. The recovery rate of enzyme activity was measured to be 83.16%.

Embodiment 3

[0053] Embodiment 3 diatomite amination and spore laccase immobilization method 3

[0054] Weigh 5g of diatomaceous earth, pour it into 75ml of absolute ethanol, add 5ml of 3-aminopropyltriethoxysilane at the same time, shake the mixture, and treat it with ultrasonic wave for 30min. The above-treated mixture was placed at 70° C. and treated at 200 r / min for 10 h. The modified diatomite treated above was repeatedly washed and collected by filtration.

[0055] Dip the diatomite prepared above into 85ml of potassium dihydrogen phosphate-dipotassium hydrogen phosphate buffer solution (pH7.0, 50mM), add 10ml of carbodiimide hydrochloride (4mg / ml) and sonicate for 10min, then add 5ml of spore suspension (100mg / ml, wet weight) was ultrasonically treated for 30min, transferred to a shaker at 200r / min, and fixed at 37°C for 12h. Wash 3 times with buffer and collect by filtration. The recovery rate of enzyme activity was measured to be 67.44%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses modified diatomite carried immobilized spore laccase as well as a preparation method and application thereof, and belongs to the technical field of immobilization and the field of wastewater treatment. The immobilized spore laccase disclosed by the invention is obtained by immobilizing spore laccase generated from a bacillus strain in the surface or pores of diatomite by virtue of an amido bond; and the preparation method comprises four steps: spore preparation, diatomite modification, immobilization and collection. The immobilized spore laccase is simple and convenient in operation, high in enzyme activity recovery rate, strong in bonding force, rich in carrier resource and low in cost, and is an ideal carrier for immobilization. The immobilized spore laccase provided by the invention is directly applicable to decoloration treatment of dyes and is relatively high in recycling capacity.

Description

technical field [0001] The invention relates to an immobilized spore laccase and its preparation and application, belonging to the technical field of immobilization and the field of wastewater treatment. Background technique [0002] Laccase is a copper-containing polyphenol oxidase. Laccase can oxidize many phenolic substances, such as monophenols, diphenols, polyphenols, aminophenols, methoxyphenols, etc., and can also react with non-phenolic substances with the assistance of mediators. Laccase can reduce oxygen to water while catalyzing the oxidation of the substrate. It does not produce harmful substances. It is a very environmentally friendly biocatalyst and can be used in the research and application of dye decolorization, beverage treatment, pulp bleaching, and biosensors. middle. [0003] Laccases are widely found in fungi, bacteria, plants and insects. Recent studies have shown that bacterial laccases are more resistant to alkali and heat than fungal laccases. B...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N11/14C02F3/34C02F101/38
Inventor 管政兵周稳廖祥儒蔡宇杰陈康
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com