Method for determining optimal picking period of capsules of paphiopedilum concolor
A technology of the same color and picking period, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of poor capsule maturity, low seed germination rate, etc. simple effect
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Embodiment 1
[0026] A method for determining the best picking period of Paphiopedilum capsules of the same color, comprising the following steps:
[0027] (1) Collection of fruit pods: pollinate the same color Paphiopedilum 4 days after flowering, and pick the fruit pods after 30-270 days of self-pollination as test materials;
[0028] (2) Fruit pod treatment: Wash the fruit pods with tap water, rinse with neutral detergent, wipe with alcohol, rinse with running water for 15 minutes, then transfer to an ultra-clean workbench, disinfect with 75% alcohol for 30 seconds, soak in 1% sodium hypochlorite solution for 12 minutes, Rinse 6 times with sterile water afterwards to obtain the treated fruit pods;
[0029] (3) Observation of inoculation and seed microstructure: cut open the treated fruit pods, take out the seeds, then inoculate a part of the seeds on the germination medium, and place the other part of the seeds under a microscope to observe the microstructure and embryo development; The...
Embodiment 2
[0033] A method for determining the best picking period of Paphiopedilum capsules of the same color, comprising the following steps:
[0034] (1) Collection of fruit pods: pollinate the same color Paphiopedilum 10 days after flowering, and pick the fruit pods as test materials after 30-270 days of self-pollination;
[0035] (2) Fruit pod treatment: Wash the fruit pods with tap water, rinse with neutral detergent, wipe with alcohol, rinse with running water for 15 minutes, then transfer to an ultra-clean workbench, disinfect with 75% alcohol for 30 seconds, soak in 1% sodium hypochlorite solution for 12 minutes, Rinse 6 times with sterile water afterwards to obtain the treated fruit pods;
[0036] (3) Observation of inoculation and seed microstructure: cut open the treated fruit pods, take out the seeds, then inoculate a part of the seeds on the germination medium, and place the other part of the seeds under a microscope to observe the microstructure and embryo development; Th...
Embodiment 3
[0040] A method for determining the best harvest period of Paphiopedilum capsules of the same color, comprising the following steps:
[0041](1) Collection of fruit pods: pollinate the same-color Paphiopedilum 6 days after flowering, and pick fruit pods after 30-270 days of self-pollination as test materials;
[0042] (2) Fruit pod treatment: Wash the fruit pods with tap water, rinse with neutral detergent, wipe with alcohol, rinse with running water for 15 minutes, then transfer to an ultra-clean workbench, disinfect with 75% alcohol for 30 seconds, soak in 1% sodium hypochlorite solution for 12 minutes, Rinse 6 times with sterile water afterwards to obtain the treated fruit pods;
[0043] (3) Observation of inoculation and seed microstructure: cut open the treated fruit pods, take out the seeds, then inoculate a part of the seeds on the germination medium, and place the other part of the seeds under a microscope to observe the microstructure and embryo development; The germ...
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