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Construction method of systemic lupus erythematosus atlas model

A lupus erythematosus, atlas model technology, applied in the fields of biochemical equipment and methods, library creation, microbial determination/inspection, etc., can solve problems such as difficult to detect

Active Publication Date: 2017-07-14
中国人民解放军联勤保障部队第九二四医院
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Problems solved by technology

And even if there is a difference, it is difficult to detect in the peripheral blood

Method used

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  • Construction method of systemic lupus erythematosus atlas model
  • Construction method of systemic lupus erythematosus atlas model
  • Construction method of systemic lupus erythematosus atlas model

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Embodiment Construction

[0048] In order to make the above objects, features and advantages of the present invention more comprehensible, specific implementations of the present invention will be described in detail below in conjunction with the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. However, the present invention can be implemented in many other ways different from those described here, and those skilled in the art can make similar improvements without departing from the connotation of the present invention, so the present invention is not limited by the specific embodiments disclosed below.

[0049] For example, a method for constructing a systemic lupus erythematosus map model includes the following steps: setting a systemic lupus erythematosus group and a normal control group, each group including several peripheral blood samples from different human bodies that have been sep...

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Abstract

A method for constructing a systemic lupus erythematosus map model, comprising the following steps: setting a systemic lupus erythematosus group and a normal control group, each group including several peripheral blood samples from different human bodies that have been separated from the human body; The DNA in the blood sample is obtained to obtain the DNA test solution; the DNA content and integrity in the DNA test solution are measured, and if the test is qualified, then the next step is performed; multiplex PCR technology is used to construct a gene library and detect the gene library , if the test is qualified, then proceed to the next step; use high-throughput sequencing technology to sequence the gene library; analyze the sequencing results to construct a systemic lupus erythematosus map model. The construction method of the systemic lupus erythematosus atlas model mentioned above is reasonable and feasible in design, and can effectively obtain SLE-related information as an intermediate result.

Description

technical field [0001] The invention relates to an acquisition of systemic lupus erythematosus information as an intermediate result, in particular to a method for constructing a systemic lupus erythematosus map model. Background technique [0002] Systemic lupus erythematosus (SLE) is a classic autoimmune disease characterized by autoantibody production, complement activation, immune complex deposition, and various tissue and organ damage. The clinical manifestations of SLE are diverse, and multiple tissues and organs such as the skin, kidney, hematopoietic system, nervous system, lung, and serosa can be involved. There are many kinds of abnormal immune functions in patients, such as decreased number and function of T cells; excessive activation and proliferation of B cells, production of various autoantibodies; dysfunction of immune complex clearance, deficiency of complement system; abnormal apoptosis and production of a large number of cytokines Triggers persistent auto...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C40B50/06C12Q1/68
Inventor 眭维国戴勇侯显良
Owner 中国人民解放军联勤保障部队第九二四医院
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