Medicago sativa MsSOS3 gene and encoding protein and application thereof

An alfalfa, gene technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problem of lack of salt-resistant, alkali-resistant, high-yield, high-quality varieties, etc., and achieve the effect of strong salt and alkali resistance

Inactive Publication Date: 2015-11-11
HARBIN NORMAL UNIVERSITY
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  • Abstract
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  • Claims
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Problems solved by technology

[0005] As a widely planted forage, alfalfa has always lacked salt-resistant, alkali-resist

Method used

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  • Medicago sativa MsSOS3 gene and encoding protein and application thereof
  • Medicago sativa MsSOS3 gene and encoding protein and application thereof
  • Medicago sativa MsSOS3 gene and encoding protein and application thereof

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Embodiment Construction

[0024] The present invention will be further described in detail below in conjunction with specific embodiments, which are explanations of the present invention rather than limitations.

[0025] 1. Cloning of MsSOS3 gene from Medicago sativa

[0026] From the NCBI database ((http: / / www.ncbi.nlm.nih.gov / ) search for the EST sequence of Medicago truncatula close relative species and save it, use the Arabidopsis thaliana AtSOS3 sequence known in the database in the BioEdit software Carry out LocalBlast to the preserved Medicago truncatula EST sequence text file, so just obtained SOS3 gene EST sequence in Medicago truncatula.Utilize this sequence to design primer, and primer is:

[0027] MsSOS3-F: 5'-TCTTGATCAATCCTTCCATC-3';

[0028] MsSOS3-R: 5'-GAATTTGTTCGATCTCTTGG-3'.

[0029] The specific process is as follows:

[0030] 1) RNA extraction: TRIZOL kit was used to extract RNA from young leaves of alfalfa (Medicagosativa), reverse transcribe it into cDNA, and use the cDNA as te...

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Abstract

The invention discloses a medicago sativa saline-alkali stress responsive gene MsSOS3 and the encoding protein and application thereof, and belongs to the technical field of gene engineering. The overall length of a gene open reading frame is 647 bp, and 215 pieces of amino acid are encoded. A nucleotide sequence shown as SEQ. ID. NO. 1 is achieved, the sequence information is used for cloning the gene, a cloning vector and a plant expression vector of the MsSOS3 gene are established, the gene is shifted into a model plant tobacco through an agrobacterium-mediated method, and the transgenic tobacco is cultivated on an MS culture medium containing saline-alkali components. Results show that a transgenic plant has high saline-alkali resistance by contrast, it means that the overexpression of the MsSOS3 gene improves the resistance of the plant to saline and alkali, and it further means that the gene can participate in the adverse resistance process of plants. The MsSOS3 gene provides a theoretical basis for studying adverse resistance molecular mechanisms and breeding of medicago sativa.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and in particular relates to an alfalfa saline-alkali stress response gene MsSOS3 and its encoded protein and application. Background technique [0002] Soil salinization is one of the important factors affecting agricultural production, and the study of plant tolerance mechanism and improvement of crop salinity tolerance have increasingly become widespread concerns. Salt-alkali stress usually causes ion imbalance, oxidative stress, osmotic stress and other damage to plants, and the high pH of alkali stress can directly cause damage to the plasma membrane of the cell. For a long time, researchers have preliminarily understood the metabolic mechanism of plants coping with saline-alkali stress by using morphological and physiological research methods, and analyzed the functions of some key genes in the above process by techniques such as gene cloning and genetic transformation. Ge...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/00
Inventor 郭长虹张军安逸民杜秉昊张雪
Owner HARBIN NORMAL UNIVERSITY
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