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Novel targeting nanoparticle as well as preparation method and application thereof

A nanoparticle and targeting technology, applied in the field of biomedicine, can solve problems such as incompleteness, and achieve the effects of prolonging survival time, improving radiosensitivity, and high stability

Inactive Publication Date: 2015-11-11
XUZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research on how to efficiently transfect small fragments of DNA molecules (length <50bp) into host cells is not yet thorough

Method used

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  • Novel targeting nanoparticle as well as preparation method and application thereof
  • Novel targeting nanoparticle as well as preparation method and application thereof
  • Novel targeting nanoparticle as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0038] The preparation method of the novel targeting nanoparticles comprises the following steps:

[0039] (1) Dissolve the positron polymer carrier PEI-CyD-FA (H1) in double-distilled water to obtain solution 1, and let it stand at room temperature for 5 minutes in the dark;

[0040] (2) Take the double-stranded DNA molecule Dbait with a hairpin-like structure of a small fragment and dissolve it in double-distilled water to obtain solution 2, and let it stand at room temperature for 5 minutes;

[0041] (3) Drop solution 1 into solution 2 in the dark and vortex, continue to vortex for 15 seconds, fully mix solution 1 and solution 2, keep in the dark and stand at room temperature for 20 minutes to obtain the new targeting nanoparticles H1 / Dbait can be used for subsequent related experiments.

[0042] The application of the novel targeted nanoparticle for tumor radiotherapy.

[0043] Further, the tumor is prostate cancer, colorectal cancer, liver cancer, lung cancer, melanoma...

Embodiment 1

[0047] Example 1. The particle size and Zeta potential detection of novel targeted nanoparticles

[0048] Take freshly prepared H1 / Dbait mixtures with different N / P ratios (1:1, 6:1, 12:1, 18:1, 24:1), and use a particle size / Zeta potential measuring instrument to measure the H1 / Dbait The light scattering particle size and Zeta potential value of the nanoparticles were measured.

Embodiment 2

[0049] Example 2. Stability and encapsulation rate detection of novel targeted nanoparticles

[0050] Take freshly prepared H1 / Dbait mixtures with different N / P ratios (1:1, 6:1, 12:1, 18:1, 24:1), and use agarose gel electrophoresis to analyze the new targeting nanoparticles H1 / Dbait's stability and package rate are tested. The specific steps of agarose gel electrophoresis are as follows:

[0051] (1) Weigh 0.5g of agarose and place it in a conical flask, add 50ml of 1×TAE solution, turn the mouth of the bottle upside down into a small beaker, heat in a microwave oven until the agarose is completely melted, and boil 3 times;

[0052] (2) When the agarose gel liquid is cooled to 60°C, add 5ul of nucleic acid dye, shake well and pour it into the spare gel-making tank of the device, so that the gel liquid is evenly spread on the bottom of the gel-making tank. Stand at room temperature until the gel is completely solidified, pull out the toothed comb in the gel-making tank ver...

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Abstract

The invention discloses a novel targeting nanoparticle as well as a preparation method and an application thereof, and belongs to the technical field of biological medicines. By a folate-targeted biodegradable positive electron polymer carrier PEI-CyD-FA (H1), double-chain DNA molecule Dbait (32bp) in a small fragment hairpin structure is encapsulated into a nanoparticle H1 / Dbait, so that the radiotherapy sensitization effect is effectively realized. The nanoparticle has the advantages of low cytotoxicity, high transfection efficiency, high stability and the like, and is convenient to prepare; the targeted nanoparticle has relatively high transfection efficiency, relatively high stability and relatively low cytotoxicity; the radiosensitivity of tumor cells can be obviously increased; the tumor growth of mice with tumors is effectively inhibited; the survival time of the mice with tumors is prolonged; and the novel targeting nanoparticle has a significant effect on tumor treatment.

Description

technical field [0001] The invention relates to a novel targeting nanoparticle loaded with a small-segment hairpin-like structure DNA molecule and its construction and application, belonging to the technical field of biomedicine. Background technique [0002] Radiation therapy is the use of one or more ionizing radiation to treat malignant tumors or some benign lesions, while causing little damage to surrounding normal tissues, and has become the main method for treating malignant tumors. Bergonie and Tribondean proposed the law of radiosensitivity in 1906. Radiosensitivity refers to radiation effects, and the radiosensitivity of tumors mainly depends on their inherent sensitivity, tissue origin, degree of differentiation, gross type, anemia, local co-infection, etc. According to the effect of radiotherapy on tumors, different tumors can be divided into radiosensitive, radiosensitive and radioresistant tumors. For radiation-resistant tumors, in the past, a palliative attit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K47/34A61P35/00
Inventor 章龙珍姚宏邱慧辛勇蒋冠邵智颖
Owner XUZHOU MEDICAL UNIV
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