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Method for preventing and treating greensickness

A technology for cotton verticillium wilt and biodegradation, which is applied in the field of prevention and control of verticillium wilt through the cooperation of agricultural biodegradable plastic film and biocontrol bacteria, can solve the problem of inability to completely and effectively solve white pollution, limited recyclable plastics, and longitudinal tearing. Low strength and other problems, to achieve the effect of low comprehensive use cost, complete degradation, uniform and thorough degradation

Active Publication Date: 2015-11-11
新疆康润洁环保科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are two main ways to solve the "white pollution" caused by plastics. One is to recycle the used plastics, but this is restricted by the use of plastics, categories, and economics. Generally speaking, recyclable plastics Limited, most of these plastics are made of petroleum, which cannot be degraded in the natural environment after use, and can also resist bacterial erosion, so the recovery rate is low, so it cannot completely and effectively solve the "white pollution" "Secondly, vigorously promote the use of degradable plastics, which is one of the effective ways to solve the problem of "white pollution"
However, most of the degradable plastic base materials are polyester polymer materials. Since the molecular arrangement of polyester is a linear arrangement during the synthesis process, when it is made into finished agricultural mulch film, the longitudinal tear strength is too low to meet the normal requirements. Therefore, the molecular chain arrangement of the molecule is changed during the synthesis, its branching degree is improved, and the "knot" effect between the molecules is effectively improved, thereby improving the physical and mechanical properties of the polyester polymer material. Meet the requirements of film laying required for normal crop growth

Method used

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  • Method for preventing and treating greensickness

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] (1) Preparation of biocontrol bacteria powder

[0045] The biocontrol bacteria-Bacillus subtilis was isolated from the rhizosphere soil of cotton. The Bacillus subtilis stored on the slant was activated on the PDA plate, and the bacteria were picked and shaken in the PD medium to collect the bacteria. The collected Bacillus subtilis was placed in 1000 mL of liquid NA medium, and cultured with shaking at a temperature of 25-28° C. and a rotation speed of 300 r / min for 40 hours to obtain a Bacillus subtilis fermentation broth. Centrifuge the fermentation broth of Bacillus subtilis at 4800r / min for 15min at room temperature, take the supernatant, mix it with 30g of macroporous resin AmberliteXAD16, vibrate at 25°C for 3h at 200r / min, and fill the column. Wash the column with distilled water first, then elute with acetone, and collect the acetone eluate. Concentrate the acetone eluent by rotary evaporation at 40° C., and finally make a powder to obtain a Bacillus subtilis...

Embodiment 2

[0054] Step (1) preparation of biocontrol bacteria powder and step (2) preparation of polyester biodegradation raw material are the same as in Example 1.

[0055] Step (3) preparation of biodegradable plastic film

[0056] Increase the biocontrol bacterium powder that contains 3%PVP in the auxiliary agent pipeline 7, wherein biocontrol bacterium powder 3.0Kg, other is identical as embodiment 1 step (3);

[0057] A polyester biodegradable mulch film for preventing and treating cotton verticillium wilt prepared above is placed in the middle of two layers of filter screens and buried 25 cm below the soil. After complete biodegradation, take out the degradation residue in the middle of the two layers . The residue was evenly sprinkled on the field with cotton, and the results showed that compared with the cotton field control without biodegradable plastic film embedded, the incidence of cotton Verticillium wilt was 7.8%, and the effective rate of disease prevention was 80%.

Embodiment 3

[0059] Step (1) preparation of biocontrol bacteria powder and step (2) preparation of polyester biodegradation raw material are the same as in Example 1.

[0060] Step (3) preparation of biodegradable plastic film

[0061] Increase biocontrol bacteria powder and seaweed fertilizer containing 3%PVP through auxiliary agent pipeline 7, wherein each 3.0Kg of biocontrol bacteria powder and seaweed fertilizer, other as embodiment 1 step (3);

[0062] A polyester biodegradable mulch film for preventing and treating cotton verticillium wilt prepared above is placed in the middle of two layers of filter screens and buried 25 cm below the soil. After complete biodegradation, take out the degradation residue in the middle of the two layers . The residue was evenly sprinkled on the field with cotton, and the results showed that, compared with the cotton field control without biodegradable plastic film embedding, the incidence of cotton verticillium wilt was 1.8%, and the effective rate o...

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Abstract

The invention relates to a method for preventing and treating greensickness through cooperation of an agricultural biodegradable mulching plastic film and biocontrol bacteria. According to the method in which cooperation between the biodegradable mulching plastic film and the biocontrol bacteria is used, the greensickness can be prevented and treated more effectively, thereby realizing higher efficiency and longer duration.

Description

technical field [0001] The invention relates to a method for preventing and treating verticillium wilt of cotton, in particular to a method for preventing and controlling verticillium wilt through the combination of agricultural biodegradable mulch and biocontrol bacteria. Background technique [0002] Cotton Verticillium wilt is a disease caused by pathogens in the soil that attack crops from the roots or stems of crops under favorable conditions. The pathogens are mainly transmitted through soil organic fertilizers, irrigation water or natural running water. Blocks can reduce crop yields by 30%-50%. [0003] Biological control is the natural control of harmful organisms by using the natural beneficial organisms in the agricultural ecosystem. The biological control of plant diseases has attracted more and more attention because of its safety, effectiveness, low toxicity, and less residue. The use of biological control is undoubtedly a radical cure. In addition, biological ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G13/02A01N63/00A01P3/00
CPCA01G9/1438A01G13/0275A01N63/00Y02A40/28
Inventor 孔琳仪孔立明任建林陈志伟
Owner 新疆康润洁环保科技股份有限公司
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