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Antinuclear antibody combined detection kit and detection method thereof

A combined detection and anti-nuclear antibody technology, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of low accuracy of detection results, large amount of samples required for detection, and long detection time, and achieves good sealing effect, satisfying Clinical use, high sensitivity effect

Inactive Publication Date: 2015-11-04
厦门拜尔杰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention provides an anti-nuclear antibody combined detection kit and its detection method to solve the problems of large amount of specimens required for the detection of existing kits, troublesome operation, long detection time, high price and low accuracy of detection results and other shortcomings

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0025] A combined antinuclear antibody detection kit, containing 20 pieces of detection cards, specifically including the following four reagents: 10mL of sample diluent, 5mL of enzyme conjugate, 10mL of washing solution and 5mL of substrate. Among them, the sample diluent includes 0.46% Tris, 0.8% Sodium Chloride, 0.1% Tween 80, 0.2% Casein, 0.1% Carboxymethylcellulose Sodium Salt, 0.04% Ethylenediaminetetraacetic acid, 0.1% Proclin-300, the rest is deionized water or distilled water; the enzyme conjugate includes 0.46% tris, 5% goat serum, 0.8% sodium chloride, 0.1% Tween 20, 0.04% ethylene glycol Amine tetraacetic acid, 0.5% sodium p-hydroxybenzoate, 0.05% HRP-labeled goat anti-human IgG, 0.1% Proclin-300, and the rest are deionized water or distilled water; the washing solution includes 0.46% sodium dihydrogen phosphate, 0.58% Disodium hydrogen phosphate dihydrate, 0.01% sodium lauryl sulfate, 0.1% Tween 80, 0.1% Tween 20, 0.04% ethylenediaminetetraacetic acid, 0.1% Procli...

Embodiment 2

[0027] An antinuclear antibody combined detection kit, containing 10 detection cards, specifically including the following four reagents: 5mL of sample diluent, 3mL of enzyme conjugate, 5mL of washing solution and 3mL of substrate. Among them, the sample diluent includes 0.46% Tris, 0.8% Sodium Chloride, 0.1% Tween 80, 0.2% Casein, 0.1% Carboxymethylcellulose Sodium Salt, 0.04% Ethylenediaminetetraacetic acid, 0.1% Proclin-300, the rest is deionized water or distilled water; the enzyme conjugate includes 0.46% tris, 5% goat serum, 0.8% sodium chloride, 0.1% Tween 20, 0.04% ethylene glycol Amine tetraacetic acid, 0.5% sodium p-hydroxybenzoate, 0.05% HRP-labeled goat anti-human IgG, 0.1% Proclin-300, and the rest are deionized water or distilled water; the washing solution includes 0.46% sodium dihydrogen phosphate, 0.58% Disodium hydrogen phosphate dihydrate, 0.01% sodium lauryl sulfate, 0.1% Tween 80, 0.1% Tween 20, 0.04% ethylenediaminetetraacetic acid, 0.1% Proclin-300, the ...

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Abstract

The invention discloses an antinuclear antibody combined detection kit which comprises a sample diluent, an enzyme conjugate, a washing fluid and a substrate. The invention also discloses a detection method using the antinuclear antibody combined detection kit. The detection method comprises balancing the detection kit to room temperature, processing a sample, rinsing a membrane surface in a card window, adding the sample and the washing fluid, adding the enzyme conjugate, developing, terminating, and the like. By using the kit and the method, multichannel multi-sample detection can be performed at the same time (that is, 16 antinuclear antibody detection projects can be performed at the same time, and 16 independent detection results can be provided), and the sensitivity can reach the detection level of an immunoblotting product and is far higher than the detection level of a dot immunogold filtration product; and the detection time of the kit is close to the detection time of dot immunogold filtration method and is far better than the detection time of an immunoblotting method, does not need reagent concentrate processing, warm bath, repeated cleaning and the like, and extremely well satisfies clinical usage.

Description

technical field [0001] The invention relates to a detection kit, more specifically to an antinuclear antibody joint detection kit and a detection method thereof. Background technique [0002] In recent years, the incidence of autoimmune diseases (AID) has shown an obvious upward trend, and its incidence accounts for 3% to 5% of the world's total population. It has become a type of disease that seriously affects human health. Autoantibodies are the most important feature of autoimmune diseases (AID), and one or more specific or related autoantibodies can be detected in the serum or other body fluids of a considerable number of AID patients. The diagnosis, differential diagnosis, disease assessment, and curative effect and prognosis judgment of AID provide very important value. The Antinuclear Antibody Spectrum (ANAs) is one of them. The diagnosis and differential diagnosis of mixed connective tissue disease (MCTD) and dermatomyositis and polymyositis (PM / DM) and other c...

Claims

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Application Information

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IPC IPC(8): G01N33/564G01N33/535
CPCG01N33/535G01N33/564
Inventor 陈隆玉史正文
Owner 厦门拜尔杰生物科技有限公司
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