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Application of phosphorescent quantum dot in selective detection of glutathione in biological fluid and wine

A technology of glutathione and quantum dots, which is applied in the direction of fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of lack of selectivity and achieve the effect of enhancing selectivity, avoiding sample pretreatment process, and exempting sample pretreatment process

Inactive Publication Date: 2015-11-04
TIANJIN NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the early days, the focus of the application of the optical properties of quantum dots in the fields of biosensing, labeling and imaging was focused on fluorescent quantum dots, but the autofluorescence of biological fluids and matrix scattered light will bring non-negligible errors, and there are also some differences in selectivity. lacking

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  • Application of phosphorescent quantum dot in selective detection of glutathione in biological fluid and wine
  • Application of phosphorescent quantum dot in selective detection of glutathione in biological fluid and wine
  • Application of phosphorescent quantum dot in selective detection of glutathione in biological fluid and wine

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Embodiment 1

[0038] 1、 Synthesis of Mn:ZnS Phosphorescent Quantum Dots

[0039] MPA, Zn(CH 3 COOH) 2 and Mn (CH 3 COOH) 2 , where MPA:Zn (CH 3 COOH) 2 : Mn (CH 3 COOH) at a molar ratio of 4:1:0.1. The pH value of the solution was adjusted to 11 with NaOH, the above mixed solution was protected by nitrogen, stirred at room temperature for 30 min, and Na 2 S and Zn (CH 3 COOH) 2 The molar ratio is 1:1 to Na 2 The S solution was quickly injected into the solution, continued to stir at room temperature for 20 min, then heated to 50 °C for 2 hours in the air with a constant temperature stirrer, added absolute ethanol, centrifuged, and vacuum-dried to obtain the Mn-doped ZnS quantum dot product.

Embodiment 2

[0041] 1、 Synthetic method with reference to embodiment 1

[0042] 2、 Phosphorescent quantum dots Mn:ZnS for the detection of glutathione in solution

[0043] (1) Add 0.01mL phosphorescent quantum dot mother solution and 1 mL Tris-HCl buffer solution to the centrifuge tube in proportion, add 0.3 μM glutathione (GSH) solution to the mixed solution, add water to make up to 10 mL, After standing for 10 minutes, phosphorescence detection was performed with a fluorescence spectrophotometer.

[0044] (2) Add 0.05 mL of phosphorescent quantum dot mother solution and 1 mL of Tris-HCl buffer solution to the centrifuge tube in proportion, add potassium permanganate solutions of different concentrations of 1 μM to the mixed solution, add water to make up to 10 mL, and statically After standing for 10 minutes, phosphorescence detection was performed with a fluorescence spectrophotometer.

[0045] (3) Add 0.01 mL phosphorescent quantum dot mother solution and 1 mL Tris-HCl buffer solut...

Embodiment 3

[0047] 1, synthetic method with reference to embodiment 1

[0048] 2. Phosphorescent quantum dot Mn:ZnS for the detection of glutathione in solution

[0049] (1) Add 0.05mL phosphorescent quantum dot mother solution and 1 mL Tris-HCl buffer solution to the centrifuge tube in proportion, add 10 μM glutathione (GSH) solution to the mixed solution, add water to make up to 10 mL, After standing for 10 minutes, phosphorescence detection was performed with a fluorescence spectrophotometer.

[0050] (2) Add 0.1 mL phosphorescent quantum dot mother solution and 1 mL Tris-HCl buffer solution in proportion to the centrifuge tube, add potassium permanganate solutions with different concentrations of 5 μM to the mixed solution, add water to make up to 10 mL, and statically After standing for 10 minutes, phosphorescence detection was performed with a fluorescence spectrophotometer.

[0051] (3) Add 0.05 mL phosphorescent quantum dot mother solution and 1 mL Tris-HCl buffer solution to th...

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Abstract

The invention discloses a method for detecting glutathione in a solution by using a phosphorescent quantum dot Mn: ZnS. According to the method, a deoxidant and an inducer are not needed in detection, and interference of background fluorescence and diffusion light can be avoided; and complex sample pretreatment is not needed in detection. The method is especially applicable to detection of glutathione in a biological fluid and a wine. The Mn: ZnS phosphorescent quantum dot prepared in the invention has good water-solubility and stability and shows good application prospects in detection of solutions or water samples.

Description

[0001] This patent is supported by the General Project 21375095 of the National Natural Science Foundation of China, the National Excellent Doctoral Dissertation Author Special Fund FANEDD-201023, the Key Project of Tianjin Applied Basic Research Program 12JCZDJC21700, and the first-level project ZX110185 of Tianjin "131" Innovative Talent Training Project funding. technical field [0002] The invention belongs to the technical field of biological analysis and detection, and relates to room-temperature phosphorescence detection of glutathione by water-phase synthesized Mn-doped ZnS quantum dots and its detection application in biological fluids and wine. Background technique [0003] In the early days, the focus of the application of the optical properties of quantum dots in the fields of biosensing, labeling and imaging was focused on fluorescent quantum dots, but the autofluorescence of biological fluids and matrix scattered light will bring non-negligible errors, and ther...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 李妍靳晴王鲁刘炳毅
Owner TIANJIN NORMAL UNIVERSITY
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