Fluorescence-labeled multiplex amplification kit for simultaneously amplifying human autosome and y-chromosome str loci and its application

Abstract

The invention discloses a fluorescence labeling combination amplification reagent kit capable of synchronously amplifying autosome gene loca and Y chromosome STR gene loca of people and application of the fluorescence labeling combination amplification reagent kit. The reagent kit can simultaneously amplify 34 gene loca to the maximum in a single tube reaction in a combination mode, wherein 34 gene loca include 15 autosome gene loca, 18 Y chromosome STR gene loca and Amelogenin. The six-color fluorescence technology is adopted, a unique gene locus arrangement mode is matched, the 34 gene loca are divided into five groups, all the groups of STR gene loca are labeled by different fluorescence labels, and in addition, the reagent kit has very high detection material adaptability. By means of the reagent kit, the autosome gene loca and the Y chromosome STR gene loca are detected simultaneously, the amplification template amount can be reduced, the detection time can be shortened, and meanwhile the efficiency of excluding and determining criminal suspects can be improved.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to a multiple amplification testing system for human autosomes and Y chromosome STR loci, in particular to a fluorescent labeling multiple amplification kit for simultaneously amplifying human autosomes and Y chromosome STR loci, It can be applied to forensic individual identification, suspect family investigation and paternity identification, etc. Background technique [0002] Short tandem repeats (Short Tandem Repeat, STR) are a type of DNA sequence with length polymorphism existing in the human genome, and its core sequence is generally composed of 2 to 6 bases, because the core sequence varies among different Length polymorphisms are present with varying numbers of tandem repeats. On average, there is one STR locus every 6-10kb in the human genome DNA. These highly polymorphic repeat sequences widely distributed in the human genome become effective genetic markers for individual...

AI Technical Summary

Problems solved by technology

At present, the Y-chromosome STR kit (Yfiler) and the autosomal STR kit (Identifiler) produced by ABI Company, which are the most widely used in China, are only used twice due to the influence of the status of the sample, the number of amplification sites, and the amplification system. The PCR process takes 5-6 hours, so the two inspection procedures often delay the best chance of catching the criminal suspect because of the increased detection time, and at the same time, the small amount of physical evidence left by the criminal suspect at the scene leads to detection Fail and eventually lose the scout lead

All in all, with the increase of criminal cases, the existing detection kits can no longer meet the requirements of public security for the rapid detection and on-site detection of forensic DNA in actual combat. How to realize the detection of autosomal STR loci and Y chromosome STR loci together , to shorten the detection time while improving the efficiency of excluding and identifying criminal suspects has become a problem to be solved

Images