Preparation method of pink tomato material

Abstract

The invention provides a preparation method of a pink tomato material, and belongs to the field of plant gene engineering. The method comprises the following steps: 1, carrying out sequence analysis on the transcription factor S1MYB12 for controlling tomato skins to determine a specific sgRNA sequence; 2, constructing an expression vector containing sgRNA and a Cas9 protein; 3, transforming the obtained recombinant vector to red tomato through an Agrobacterium infection technology to obtain T0 generation transgenic tomato plants; and 4, planting the T0 generation plants with S1MYB12 gene sequence mutation in a greenhouse, selfing to obtain T1 generation plants, and screening plants free from exogenous transgenic components, with S1MYB12 gene sequence homozygous mutation and with pink fruits from the T1 generation plants in order to obtain the pink tomato material. The method has the advantages of low cost, preparation cycle shortening, and obtaining of the pink tomato material within one year; and the pink tomato does not contain exogenous gene, is safe and is free from risk.

Description

technical field [0001] The invention belongs to the fields of plant genetic engineering and genetic modification, and in particular relates to a method for preparing powdery tomato materials using a CRISPR / Cas9 system. Background technique [0002] Tomato is one of the most important vegetable crops widely grown worldwide. Fruit color is an important economic trait of tomato, and consumers in different regions have different preferences for tomato fruit color. In Europe and North America, consumers prefer red tomatoes, while consumers in Asia prefer pink tomatoes. Tomato commercial breeding in the past few decades has mainly focused on the improvement of red-fruited tomatoes, and there are relatively few excellent pink-fruited tomatoes. At present, Chinese breeders mostly focus on cultivating pink fruit resources with red fruit background to meet the needs of consumers. [0003] The difference between red and pink tomatoes is mainly determined by the flavonoids in the pee...

AI Technical Summary

Problems solved by technology

[0004] It takes at least 3-4 years to transform an excellent red-fruited tomato inbred line into a pink-fruited tomato inbred line through the traditional backcrossing method, including one crossing, at least five backcrossings and one selfing, which is time-consuming and labor-intensive

Images