Gas-producing photoacoustic imaging contrast agent and preparation method thereof
A photoacoustic imaging and contrast agent technology, applied in the field of clinical medicine, can solve problems that cannot be ruled out, harmful to the body, and expensive, and achieve the effects of low cost, easy source, and improved diagnostic value
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Embodiment 1
[0036] Example 1 Preparation of photoacoustic imaging contrast agent
[0037] 1. Selection of main materials: polyethylene glycol-distearoylphosphatidylethanolamine (DSPE-PEG), dipalmitoylphosphatidylcholine (DPPC), cholesterol, ammonium bicarbonate (NH 4 HCO 3 ).
[0038] 2. Preparation of photoacoustic imaging contrast agent: Ammonium bicarbonate lipid microspheres were prepared by thin film hydration method and extrusion method. Liposome microspheres were filled with ammonium bicarbonate saturated solution, outsourcing lipid, and then removed by dialysis Ammonium bicarbonate outside the liposomes to obtain liposome microspheres.
[0039] 3. Preparation steps:
[0040] Dissolve 10mgDPPC, 1mgDSPE-PEG, and 1mg cholesterol in 10ml chloroform. After fully dissolving to a clear and transparent degree, put it on a rotary evaporator and rotate it at a temperature of 45°C, for 50 minutes, and a rotation speed of 60r / min. The obtained phospholipid membrane was added with 5ml of a...
Embodiment 2
[0041] Example 2 Preparation of photoacoustic imaging contrast agent
[0042] 1. Selection of main materials: polyethylene glycol-distearoylphosphatidylethanolamine (DSPE-PEG), dipalmitoylphosphatidylcholine (DPPC), cholesterol, ammonium bicarbonate (NH 4 HCO 3 ).
[0043] 2. Preparation of photoacoustic imaging contrast agent: Prepare ammonium bicarbonate lipid microspheres (ammonium bicarbonate saturated solution inside, lipid outsourcing) by thin film hydration method and extrusion method, and then remove the liposomes by dialysis. ammonium bicarbonate to obtain liposome microspheres.
[0044] 3. Preparation steps:
[0045] Step 1: Dissolving phospholipids: Add 1mgDSPE-PEG, 10mgDPPC and 1mg cholesterol to 10ml chloroform, shake it by hand until fully dissolved, and form a clear mixed solution of phospholipids and chloroform;
[0046] Step 2: Prepare phospholipid film: place the mixed solution obtained in step 1 on a rotary evaporator to rotate at a speed of 40r / min, time:...
Embodiment 3
[0049] Example 3 Preparation of photoacoustic imaging contrast agent
[0050] 1. Selection of main materials: polyethylene glycol-distearoylphosphatidylethanolamine (DSPE-PEG), dipalmitoylphosphatidylcholine (DPPC), cholesterol, ammonium bicarbonate (NH 4 HCO 3 )
[0051] 2. Preparation of photoacoustic imaging contrast agent: Prepare ammonium bicarbonate lipid microspheres (ammonium bicarbonate saturated solution inside, lipid outsourcing) by thin film hydration method and extrusion method, and then remove the liposomes by dialysis. ammonium bicarbonate to obtain liposome microspheres.
[0052] 3. Preparation steps:
[0053] Step 1: Dissolving phospholipids: Add 1mgDSPE-PEG, 10mgDPPC and 1mg cholesterol to 10ml chloroform, shake it by hand until fully dissolved, and form a clear mixed solution of phospholipids and chloroform;
[0054] Step 2: Preparation of phospholipid film: Place the mixed solution obtained in step 1 on a rotary evaporator to rotate at a speed of 80 r / m...
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