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Process for separating butanol fermentation liquor by using continuous chromatography

A technology for separating butanol and continuous chromatography, applied in the field of extracting butanol, can solve the problems of increasing the cost of ABE separation process, low total recovery rate of butanol, low adsorption capacity, etc., achieving low operating cost, improving equipment utilization, The effect of low wastewater discharge

Active Publication Date: 2015-10-14
NANJING UNIV OF TECH
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  • Description
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Problems solved by technology

However, the adsorption capacity is low and the overall recovery of butanol is low
Liu et al. (Liu F, Liu L, Feng X. Separation of acetone-butanol-ethanol (ABE) from dilute aqueous solutions by pervaporation) used polyether block amide copolymer membranes to separate ABE fermentation broth, but pervaporation separated biotin Alcohol is greatly affected by the properties of the membrane material itself, and the cost is high
DIJK (WO 2008 / 095896A1) and others use a microporous resin with an ultra-high crosslinking degree to separate bio-butanol, but the resin has a certain amount of adsorption to acetone and ethanol at the same time, which increases the cost of the later ABE separation process

Method used

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  • Process for separating butanol fermentation liquor by using continuous chromatography
  • Process for separating butanol fermentation liquor by using continuous chromatography
  • Process for separating butanol fermentation liquor by using continuous chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: Obtaining of ABE fermentation broth.

[0046] Obtained from the fermentation of Clostridium acetobutylicum B3 (CGMCC NO.5234, recorded in Chinese patent CN103320335A), its medium composition: carbon source and nitrogen source: 60g / L Glucose, 2.2g / L CH 3 COONH 4 , 1.0g / L corn steep liquor; metal ions: 0.01g / L Nacl, 0.2g / L MgSO 4 , 0.01g / L MnSO 4 , 0.01g / L FeSO 4 ;Buffer system: 0.5g / L KH 2 PO 4 , 0.5g / L K 2 HPO 4 ;Vitamins: p-aminobenzoic acid 1mg / L, thiamine 1mg / L, biotin 0.01mg / L; carbon source, nitrogen source and buffer system were sterilized at 121°C for 15min, then cooled to 37°C, metal ions and vitamins Sterilize by filtration through a 0.22 μm membrane filter. The medium of the inoculated strains was passed through N 2 2-7min, maintain anaerobic environment, anaerobic culture at 37°C for 50h.

[0047] The obtained fermented liquid is subjected to coarse filtration first, and ultrafiltration is carried out with an ultrafiltration membrane w...

Embodiment 2

[0048] Example 2: Continuous chromatographic separation of ABE fermentation broth.

[0049] A continuous separation system consisting of 10 resin columns ( figure 1 ), the adsorption section is 5, the desorption section is 3, and the regeneration section is 2. Each resin column is filled with 90 g of resin (KA-I), the diameter of the resin column is 3.0 cm, and the height is 24 cm. The pretreated ABE fermentation broth is put on the column, the concentration of the column: butanol 12g / L, ethanol 2g / L, acetone 6g / L, the adsorption flow rate is 14BV / h, and the adsorption capacity is 0.14g / g wet resin; , empty the feed liquid in the void volume of the resin bed at a flow rate of 1.0BV / h, and circulate the discharged feed liquid to the feed liquid to re-enter the adsorption section for adsorption. After the resin column is emptied and before entering the desorption section, soak the resin with the collected product solution at a flow rate of 1.0BV / h, and then start desorption. ...

Embodiment 3

[0050] Example 3: Continuous chromatographic separation of ABE fermentation broth.

[0051] A continuous separation system consisting of 12 resin columns ( figure 2 ), the adsorption section is 6, the desorption section is 3, and the regeneration section is 3. Each resin column is filled with 80 g of resin (KA-I), the diameter of the resin column is 3.0 cm, and the height is 22 cm. The pretreated ABE fermentation broth is put on the column, the concentration of the column: butanol 12g / L, ethanol 2g / L, acetone 6g / L, the adsorption flow rate is 16BV / h, and the adsorption capacity is 0.14g / g wet resin; , the feed liquid in the void volume of the resin bed is emptied at a flow rate of 1.2BV / h, and the discharged feed liquid is circulated to the feed liquid to re-enter the adsorption section for adsorption. After the resin column is emptied and before entering the desorption section, soak the resin with the collected product solution at a flow rate of 1.2BV / h, and then start des...

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Abstract

The invention discloses a process for separating butanol fermentation liquor by using continuous chromatography. The butanol fermentation liquor is ABE (Acetone-Butanol-Ethanol) fermentation liquor. The process is characterized by comprising the steps of carrying out filtration and ultrafiltration treatment on the butanol fermentation liquor, then, pumping the obtained clear liquid into a continuous separating device containing a hydrophobic macroporous polymer adsorbent for adsorption, carrying out desorption by using ethanol as a desorbent so as to obtain a product butanol, and carrying out adsorbent regeneration by using pure water as a regenerant. According to the process, the aim of separating and purifying butanol is realized through the continuous device by using adsorption and elution competition actions among three ingredients, i.e., acetone, ethanol and butanol, the content of butanol in an upper layer of product liquid can reach over 29-32wt%, the purity of butanol is over 91%, and the average yield is 99%; and the volume of an extracting solution is reduced, a solvent in the extracting solution is self-produced ethanol or acetone in the butanol fermentation liquor, and no other ingredient is added during extraction, so that a large number of energy is saved to further recover various ingredients through the traditional rectification method.

Description

technical field [0001] The invention belongs to the field of biotechnology processing, and in particular relates to a method for extracting butanol from butanol fermentation liquid by using continuous chromatography technology. Background technique [0002] Butanol (n-butanol or 1-butanol) and its derivatives are important chemical raw materials, widely used in food, medicine, agriculture, chemical energy and other fields. Butanol is mainly used in the production of butyl acrylate and butyl methacrylate plasticizers in various plastic and rubber industries, accounting for about 40% of the total butanol consumption; butanol can also be used in the synthesis of drugs, Such as antibiotics, vitamins and hormones, etc., and as the extractant of spices, the best diluent for brake fluid. Because the energy density, air-fuel ratio, heat of vaporization and octane number of butanol are close to those of gasoline, it can be mixed with gasoline in a certain proportion without complete...

Claims

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Application Information

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IPC IPC(8): C07C29/76C07C31/12
CPCC07C29/76C07C31/12
Inventor 应汉杰吴菁岚柯旭周精卫庄伟陈勇
Owner NANJING UNIV OF TECH
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