A kind of spore-producing medium of corn leaf spot fungus and its preparation method and application

A technology of corn spot disease bacteria and medium, applied in the field of plant pathogenic fungus research, can solve problems such as complex operation process, achieve good growth, reduce unnecessary links, and low production cost

Active Publication Date: 2018-04-24
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using the water agar plate surface germination method to carry out fungicide inhibition of pathogenic fungus spore germination test, usually requires a corresponding concentration of liquid medicine to prepare the spore suspension, and then coat the surface of the plate containing the drug, the operation process is more complicated than the concave glass slide method

Method used

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  • A kind of spore-producing medium of corn leaf spot fungus and its preparation method and application
  • A kind of spore-producing medium of corn leaf spot fungus and its preparation method and application
  • A kind of spore-producing medium of corn leaf spot fungus and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: the influence of different mediums on the growth of pathogen mycelium, sporulation

[0026] Tested strain: corn leaf spot fungus FJ.

[0027] Test medium: mannitol medium, potato dextrose medium (PDA medium), potato sucrose medium (PSA medium) and 1.4% water agar medium.

[0028] Wherein the preparation method of mannitol medium is as follows:

[0029] Weigh and wash the potatoes, cut them into small pieces, add water to boil for 0.5h, filter the potatoes with gauze, add water to make up to 1L, divide them into triangular flasks, then add mannitol and agar powder in proportion, sterilize at 121°C for 25min, and wait for the medium to cool At about 50°C, pour into a petri dish with a diameter of 8.9cm, about 25ml per dish, and set aside. The quality of raw materials for preparing different concentrations of mannitol medium is shown in Table 1:

[0030] The quality of each raw material of table 1 mannitol medium

[0031]

Potatoes (g)

Aga...

Embodiment 2

[0043] Example 2: Application of spores cultivated in mannitol medium in drug screening

[0044] Tested strain: corn leaf spot fungus FJ.

[0045] The test agents: pyraclostrobin technical (BASF, Germany), iprodione technical (Jiangsu Huifeng Co., Ltd.) and fluazinam technical (Lier Chemical Co., Ltd.).

[0046] Test medium: medium containing 0.5% mannitol and 1.4% water agar medium.

[0047] Preparation of drug-containing medium: On the basis of preliminary experiments, water agar media with different concentrations of drugs in Table 4 were prepared respectively, and the medium without drug was used as a control, and repeated 3 times (3 dishes).

[0048] Test method: use 0.5% mannitol medium to cultivate the pathogenic bacteria for 6-7 days, then cover the surface of the culture medium with the same bacterial age with a sterile filter paper square (about 1.5×1.5cm in size), after the filter paper is wet, Move the filter paper to the surface of the water agar medium containi...

Embodiment 3

[0054] Embodiment 3: the application of the spore cultivated in mannitol medium in pathogenicity determination of pathogen

[0055] Tested strain: Corn spot fungus FJ

[0056] Tested corn variety: T1402

[0057] Test medium: 0.5% mannitol medium, 1.0% mannitol medium, PDA medium and PSA medium.

[0058] Test method: After the fungus has been activated, use an inoculation needle to inoculate the germ cake into the center of the medium plate, with the mycelium facing down, and culture in the dark at 28°C for 7 days (repeat this operation for subculture) to obtain a large number of meristems After spores, inject clean water on the surface of the culture medium, scrape and wash the spores on the surface of the culture medium with a sterile glass slide, filter with double-layer sterile gauze, and dilute the spore suspension with clean water to make the final concentration of conidia 1-10× 10 5 per mL, spray the spore suspension on corn plants (5-6 leaf stage), cover the inoculat...

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Abstract

The invention discloses a spore-producing medium for corn leaf spot fungus, a preparation method and application thereof. The medium includes potatoes, mannitol, agar powder and water, wherein 200 g / L of potatoes, 5-10 g / L of mannitol, and 14 g / L of agar powder. Cultivated on the surface of the spore-forming medium, and cultivated in the dark at 28°C for 6-7 days, a large number of conidia with uniform shape, good germination effect, and high germination rate can be produced on the surface of the medium, and the sporulation yield is about equal to 1.2 to 3.6 times the sporulation yield of PDA medium, and the germination rate can reach more than 90%. The spore-producing culture medium for the corn leaf spot fungus is suitable for the subculture of the pathogen, and can be used in the screening of the pathogenic agent and the determination of the pathogenicity of the pathogen.

Description

technical field [0001] The invention relates to the technical field of research on plant pathogenic fungi, in particular to a spore-producing medium for spore-forming fungi of maize spot and its preparation method and application. Background technique [0002] Southern corn leaf blight is an important disease in corn production, widely distributed in my country. Corn blight is an airborne disease. The conidia produced by P. maize play an important role in the spread, infection and pathogenicity of the pathogen. The conidia can be spread to the corn leaves growing in the field by means of airflow or rain. When there are free water droplets, the conidia The spores can germinate in 4-8 hours to produce germ tubes and invade the epidermal cells of corn leaves, and lesions can be formed within 3-4 days, and then the lesions can produce conidia, which spread with the help of airflow and repeatedly infect corn grown in the field . At present, using the disease resistance of crop...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N3/00C12N1/14C12Q1/04C12R1/645
Inventor 杨秀娟陈福如甘林阮宏椿杜宜新石妞妞
Owner INST OF PLANT PROTECTION FAAS
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