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Active peptide for treating cancer and application of active peptide

A technology of usage and homology, applied in the direction of peptides, drug combinations, anti-tumor drugs, etc., to achieve good stability

Inactive Publication Date: 2015-10-07
查文娟
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no reports on medical devices for early diagnosis of tongue cancer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Separation and Obtaining of Polypeptides

[0039] (1) Wash the cone snails with PBS buffer solution, then use 13mmol / L HCl solution to homogenate and prepare a crude solution at 10 degrees Celsius; (2) process the crude solution obtained in step (1) with 8000 rpm Centrifuge for 20 minutes, collect the supernatant, filter the supernatant with three layers of gauze, then add 1 kg of papain (enzyme activity is 800AU / g), continue to incubate and stir for 3 hours to obtain the enzymolysis solution. Separate with Sephadex G-50 (1.5cm×90cm), elute with 19mmol / L HCl solution, flow rate 1.3mL / min, collect the eluted product, adjust the solution to pH 6.8, 10000 rpm Minutes centrifuged for 15 minutes, and the supernatant was freeze-dried for later use; after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), small molecular weight bands were recovered, and 40 small polypeptides corresponding to them were obtained by sequencing. the sequence of amino ...

Embodiment 2

[0040] Example 2 Using the MTT method to evaluate the growth inhibitory effect of polypeptides on human tongue cancer cell lines

[0041] Method: Cells in the logarithmic phase of growth: Human tongue cancer cell lines Tca8113 and T6 were treated with 2×I0 4 Concentrations were seeded in 96-well plates. After 24 h of cell culture, the original cell culture medium (RPMI-1640 medium) was sucked off. The experiment was divided into blank control group and drug treatment group. The blank group was replaced with 1640 medium containing 10% fetal bovine serum; the drug-treated group was replaced with a cell culture medium containing a peptide concentration of 50 μM. After culturing for 48 h, add MTT at a concentration of 5 mg / mL, and continue to store in CO 2 After culturing in an incubator for 4 h, 100 μL of supernatant was sucked along the upper part of the culture medium, 100 μL of DMSO was added, and placed in the dark for 10 min, and the absorbance value (wavelength 570 nm) w...

Embodiment 3

[0045] Example 3 Stability experiment of polypeptide in mice

[0046]The polypeptide was injected into mice at a dose of 0.5 mg / ml / kg. After a week of in vivo metabolism, the tail blood of the mice was taken and analyzed by SDS-PAGE. Most of the residual polypeptides in the blood were at a concentration of It can still reach an average of 0.2 mg / ml / kg, which fully demonstrates that the polypeptides have good stability in vivo and can be used for subsequent pharmaceutical purposes.

[0047] The above results clearly demonstrate that the present invention provides a disease that can be used to treat cancer. Has a better prospect for the treatment of cancer.

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PUM

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Abstract

The invention provides a series of peptides for resisting tongue cancer and a method for extracting the peptide. The series of peptides are higher in stability and capacity for suppressing growth of tongue cancer cells. The peptides can be used for treating cancers.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an active peptide isolated from cone snails for treating tongue cancer. Background technique [0002] Cancer is one of the most harmful diseases to human life and health, and a large number of people die of cancer every year. The research and development of anticancer drugs has always been a hot spot in pharmaceutical research. 74% of anti-tumor drugs are natural products or their derivatives, such as paclitaxel and its derivatives are anti-tumor drugs with better clinical application effect at present. Therefore, it is of great significance to search for anticancer compounds or lead compounds from natural products. [0003] Tongue cancer can be manifested in three types in the early stage: ulcer, exogenous and infiltrating. In some cases, the first symptom is only tongue pain, which can sometimes be reflected to the temple or ear. The exophytic type can arise from malignant tran...

Claims

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Application Information

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IPC IPC(8): C07K7/08A61K38/10A61P35/00
Inventor 查文娟
Owner 查文娟
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