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Chitosan-Nisin core-shell microspheres and preparation method and application thereof

A technology of chitosan and chitosan solution, which is applied in the direction of preserving meat/fish with chemicals, can solve the problems of chemical preservative residue, high temperature setting requirements, and large investment in supporting facilities, etc., to achieve enhanced antibacterial activity, Effect of maintaining nutritional and sensory properties, good biocompatibility

Inactive Publication Date: 2015-09-23
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, low-temperature preservation has disadvantages such as high temperature setting requirements and large investment in supporting facilities, which limit its application in aquatic products
The use of chemical preservatives makes microorganisms resistant to drugs and affects the antibacterial effect, and chemical preservatives will remain in aquatic products, posing safety hazards

Method used

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  • Chitosan-Nisin core-shell microspheres and preparation method and application thereof
  • Chitosan-Nisin core-shell microspheres and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: This example prepares chitosan-Nisin core-shell microspheres.

[0024] Accurately weigh 1.000g of chitosan and dissolve in 900ml of pure water, add 9ml of glacial acetic acid to fully dissolve, stir, and sonicate for 10min. Accurately add 1.000g of Nisin to the upward solution, and after mixing evenly, add 100ml of 0.5M trisodium citrate solution with an infusion pump at a speed of 0.5ml / min. Add 5ml of Tween 80 as a non-ionic stabilizer, and stir for 2h under a magnetic stirrer. The resulting suspension was centrifuged at 6000 r / min at 4° C. for 10 minutes, the supernatant was discarded, and the resulting precipitate was placed in a -80° C. refrigerator and freeze-dried to obtain chitosan-embedded Nisin particles.

[0025] Test result: measure the content of Nisin in the supernatant that step 2 discards with high performance liquid phase method, obtain the embedding rate of Nisin 58%; By laser particle size analyzer, record the average particle diameter of ...

Embodiment 2

[0027] Example 2: This example prepares chitosan-Nisin core-shell microspheres.

[0028] Accurately weigh 3.000g of chitosan and dissolve in 900ml of pure water, add 18ml of glacial acetic acid to fully dissolve, stir, and sonicate for 10min. Accurately add 1.500g Nisin to the upward solution, and after mixing evenly, add 0.5M sodium sulfate solution at a rate of 2ml / min with an infusion pump. Then add 10ml Tween 80 as a non-ionic stabilizer and stir for 3.5h under a magnetic stirrer. The resulting suspension was centrifuged at 8000 r / min at 4° C. for 10 minutes, the supernatant was discarded, and the resulting precipitate was placed in a -80° C. refrigerator and freeze-dried to obtain chitosan-embedded Nisin particles.

[0029] Test results: The inclusion compound prepared by this formula has a Nisin embedding rate of 62% and an average particle size of 930±45.8nm.

[0030] Referring to the usage limit of Nisin in food additives and other factors, 0.75 g of the core-shell m...

Embodiment 3

[0031] Example 3: This example prepares chitosan-Nisin core-shell microspheres.

[0032] Accurately weigh 3.000g of chitosan and dissolve in 900ml of pure water, add 13.5ml of glacial acetic acid to fully dissolve, stir, and sonicate for 10min. Accurately add 1.000g of Nisin to the upward solution, and after mixing evenly, add 100ml of 0.5M trisodium citrate solution at a rate of 6ml / min with an infusion pump. Then 8ml of Tween 80 was added as a non-ionic stabilizer. Stir for 5 h under a magnetic stirrer. The resulting suspension was centrifuged at 8000 r / min at 4° C. for 10 minutes, the supernatant was discarded, and the resulting precipitate was placed in a -80° C. refrigerator and freeze-dried to obtain chitosan-embedded Nisin.

[0033] Test results: The inclusion compound prepared by this formula has a Nisin embedding rate of 61% and an average particle size of 677±35.6nm.

[0034] Referring to the usage limit of Nisin in food additives and other factors, 1.0 g of the c...

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Abstract

The invention discloses chitosan-Nisin core-shell microspheres and a preparation method and application thereof. The chitosan-Nisin core-shell microspheres are made from core-shell materials wherein chitosan coats Nisin. Preparation of the material comprises embedding lactic streptococci by using chitosan under an acidic condition to form a clathrate compound so as to reduce the spatial resistance of the material, improve the stability of the material, enhance the antibacterial activity and reduce the environmental influence, so that the function of the material can be ensured in different environments. Moreover, the chitosan-Nisin core-shell microspheres have the advantages of good biocompatibility, hypotoxicity, biodegradability and the like, and are quite suitable for fresh-keeping of aquatic products (the fresh-keeping condition is neutral or alkaline), particularly yellow croakers, silvery pomfret and hairtail.

Description

technical field [0001] The invention relates to a chitosan-Nisin core-shell microsphere suitable for fresh-keeping of aquatic products and a preparation method thereof, and the chitosan-Nisin core-shell microsphere is used for fresh-keeping of fish. Background technique [0002] Chitosan is widely used, and it is increasingly used in biomedicine, including drug delivery, gene delivery, wound healing, antibacterial applications, tissue engineering, and vaccine delivery. The many applications of chitosan are attributed to its excellent functional versatility, which can be utilized in the form of solution, gel and nano or microparticles. Chitosan nano / microparticle system can be used to promote special functions such as gene, protein biological preparation and antigen delivery. When developing chitosan nano / microparticle systems for drug or protein delivery, it is very important to control the characteristics of particle size, particle size distribution, surface charge, etc., ...

Claims

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Application Information

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IPC IPC(8): A23B4/20
Inventor 胡亚芹陈士国丁甜陈健初吴甜甜吴春华符莎露
Owner ZHEJIANG UNIV
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