Determination method for glycine betaine in wolfberry fruits

A determination method and betaine technology, applied in the field of detection and analysis, can solve the problems of not meeting the needs of sample detection, affecting the results of quantitative analysis, not suitable for betaine determination, etc., to avoid the use of toxic and harmful organic solvents, high-sensitivity determination, Satisfy the detection effect

Inactive Publication Date: 2015-09-16
JINAN INST OF FRUIT PRODS CHINA GENERAL SUPPLY & MARKETING COOP
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Problems solved by technology

These two standards both use ion chromatography, but they are not suitable for the determination of betaine in samples with high sugar content, and there is interference of sodium ions in the determination process, which seriously affects the quantitative analysis results
In addition, other standards involving the determination of betaine have complex operations and high detection limits, such as NY / T1746-2009 "Determination of Betaine in Beetroot", the detection limit is 400mg / kg, which is far from meeting the actual sample detection needs

Method used

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  • Determination method for glycine betaine in wolfberry fruits
  • Determination method for glycine betaine in wolfberry fruits
  • Determination method for glycine betaine in wolfberry fruits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The selection of embodiment 1 ion chromatography analysis chromatographic conditions

[0029] (1) Selection of chromatographic column

[0030] The experiment compares the commonly used IonPac CS17 chromatographic column and IonPac CS19 chromatographic column. The results showed that when the IonPac CS17 column was used for analysis, the chromatographic peaks of other impurities in the wolfberry sample could not be completely separated from the chromatographic peaks of betaine; when the IonPac CS19 column was used, the separation effect was significantly better than that of IonPac CS17, and the retention time of betaine was stable. The relative standard deviation of retention times was less than 1.1% over 123 days.

[0031] (2) Column temperature and analysis time conditions

[0032] When the temperature is maintained at 30°C, the best column efficiency is obtained, complete separation from adjacent miscellaneous peaks, high resolution, and good separation effect.

[...

Embodiment 2

[0034] Determination experiment of betaine in embodiment 2 wolfberry samples

[0035] This method was applied to the determination of betaine in wolfberry samples.

[0036] (1) Sample pretreatment

[0037] Weigh about 0.5g of the pulverized solid sample (the solid sample is Lycium barbarum) or about 2.5g of the liquid sample, weigh it precisely, add water to dissolve it and set the volume to 25ml, ultrasonically treat (500W / 40KHz) for 30min, and centrifuge for 10min (speed 10000rpm ), take the supernatant and filter the membrane, pass through the pre-activated RP column, discard the first 3mL, take the continued filtrate, and put it on the machine for testing.

[0038] (2) Configuration of standard solution

[0039] Betaine standard stock solution 1000mg / L: Accurately weigh 0.1000g of the standard substance, dissolve it in pure water, shake well and set aside. Then gradually dilute to the required concentration. Standard substance chromatogram (chromatographic condition is...

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Abstract

The invention discloses a determination method for glycine betaine in wolfberry fruits. The method comprises the following steps: (1) extracting wolfberry fruit samples with a water extracting solvent, and taking a filtering solution obtained by filtering as a solution to be detected; and (2) analyzing and determining glycine betaine in the samples by combining an ion chromatography with a pulse ampere detector. The invention establishes the method for determining glycine betaine in the wolfberry fruits by combining the ion chromatography with the pulse ampere detector. The method has good linearity; and the recycling rate is 90.8%-94.7%, the accuracy is 1.11% and the detection limit is 0.0366 micrograms / milliliter. According to the method, a toxic reagent is not used; and a test verifies that the method is applicable to rapid and batch detection of glycine betaine in various phytophagous and functional foods.

Description

technical field [0001] The invention relates to an analysis method for determining betaine in wolfberry, belonging to the technical field of detection and analysis. Background technique [0002] Lycium barbarum (medlar) is the dried fruit of a perennial deciduous shrub belonging to the family Solanaceae. Modern pharmacological research generally believes that the active ingredients and trace elements of wolfberry have pharmacological effects. There are three main types of active ingredients in wolfberry, and betaine is one of them. Betaine is a kind of quaternary ammonium compound, molecular formula C 5 h 11 NO 2, Structural formula (CH 3 ) 3 N + -CH 2 -COO - , relative molecular weight 117.15. It is one of the main alkaloids in the fruits, stalks and leaves of wolfberry, and it acts as a methyl supplier in plants. At present, there are more and more studies on the determination methods of betaine content, mainly including titration, colorimetry, high performance ...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 闫新焕宋烨朱风涛刘雪梅郑晓冬孟晓萌潘少香李仁勇
Owner JINAN INST OF FRUIT PRODS CHINA GENERAL SUPPLY & MARKETING COOP
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