Method for producing alpha-arbutin by virtue of biological fermentation

A technology of biological fermentation and arbutin, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of high production cost, complicated synthesis process, and not very deep arbutin, and achieve good industrialization Easy production and process control and low production cost

Active Publication Date: 2015-09-16
FENCHEM BIOTEK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are many studies on α-arbutin at home and abroad, among which the research on arbutin abroad is relatively sufficient, and it has been applied in the field of cosmetics. The domestic research on arbutin is not very in-depth. The method of preparing arbutin is mainly through chemical synthesis, but the method of chemical synthesis cannot produce α-arbutin, and the domestic production of arbutin is only in the early stage. In the process of research, there is no report that large-scale fermentation production can be successfully carried out
The Chinese patent with application number 201110431955.9 discloses a method for catalyzing the synthesis of α-arbutin by using lipase, which can catalyze the synthesis of α-arbutin by Candida lipase, but its synthesis process is relatively complicated, and for large-scale The production is not easy to control, and the production cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A method for producing α-arbutin by biological fermentation, the steps are as follows:

[0031] Step 1, adding 10 kg of wheat flour to water for liquefaction, specifically adding 100 kg of water to the wheat flour, stirring and mixing evenly, then heating to 80° C., and stirring for 30 minutes to obtain liquefied flour;

[0032] Step 2, adding bran, calcium carbonate, potassium dihydrogen phosphate and urea to the liquefied flour, adding 3 kg of bran, 0.4 kg of calcium carbonate, 0.2 kg of potassium dihydrogen phosphate, and disodium edetate 0.1kg, add urea 0.1kg, stir and mix evenly to obtain a mixed matrix;

[0033] Step 3, insert Aspergillus oryzae into the beef extract peptone medium, the inoculum size is 0.6%, and cultivate and activate at 40-42°C for 5 hours;

[0034] Step 4, insert the Aspergillus oryzae in step 3 into the mixed matrix of step 2, the inoculum amount is 0.3%, cultivate for 3 hours under the condition of 38-40°C, then add hydroquinone into the mix...

Embodiment 2

[0040] A method for producing α-arbutin by biological fermentation, the steps are as follows:

[0041] Step 1, adding 15kg of wheat flour to water for liquefaction, specifically adding 180kg of water to the wheat flour, stirring and mixing evenly, then heating to 82°C, and stirring for 35 minutes to obtain liquefied flour;

[0042] Step 2, adding bran, calcium carbonate, potassium dihydrogen phosphate and urea to the liquefied flour, adding 3kg of bran, 0.6kg of calcium carbonate, 0.45kg of potassium dihydrogen phosphate, and disodium edetate 0.3kg, add 0.3kg of urea, stir and mix evenly to obtain a mixed matrix;

[0043] Step 3, insert Aspergillus oryzae into beef extract peptone medium, the inoculum size is 0.7%, and cultivate and activate at 40-42°C for 7 hours;

[0044] Step 4, insert the Aspergillus oryzae in step 3 into the mixed matrix of step 2, the inoculum amount is 0.4%, cultivate for 4 hours under the condition of 38-40°C, then add hydroquinone in the mixed matrix...

Embodiment 3

[0050] A method for producing α-arbutin by biological fermentation, the steps are as follows:

[0051] Step 1, adding 12 kg of wheat flour to water for liquefaction, specifically adding 160 kg of water to the wheat flour, stirring and mixing evenly, then heating to 83° C., and stirring for 36 minutes to obtain liquefied flour;

[0052] Step 2, adding bran, calcium carbonate, potassium dihydrogen phosphate and urea to the liquefied flour, adding 6 kg of bran, 0.6 kg of calcium carbonate, 0.48 kg of potassium dihydrogen phosphate, and disodium edetate 0.2kg, add urea 0.36kg, stir and mix evenly to obtain a mixed matrix;

[0053] Step 3, insert Aspergillus oryzae into the beef extract peptone medium, the inoculum amount is 0.8%, and cultivate and activate at 40-42°C for 8 hours;

[0054] Step 4, insert the Aspergillus oryzae in step 3 into the mixed matrix of step 2, the inoculum amount is 0.5%, cultivate it at 38-40°C for 5 hours, then add hydroquinone into the mixed matrix, an...

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PUM

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Abstract

The invention discloses a method for producing alpha-arbutin by virtue of biological fermentation, wherein liquefied flour, bran, calcium carbonate, monopotassium phosphate and urea are used as a fermentation substrate, and hydroquinone is added in the fermentation substrate and fermented by aspergillus oryzae to prepare alpha-arbutin. The method specifically comprises the following steps: adding wheat flour in water and liquefying, and then adding bran, calcium carbonate, monopotassium phosphate, ethylenediamine tetraacetic acid disodium salt and urea; inoculating the activated aspergillus oryzae in the solution, culturing for a period of time under the condition of 38-40 DEG C, adding hydroquinone, uniformly mixing, keeping a fermentation temperature at 42-45 DEG C, and fermenting for a certain time, thereby obtaining a fermentation mixture; and finally separating and purifying the fermentation mixture to obtain alpha-arbutin. The method for producing alpha-arbutin by virtue of biological fermentation provided by the invention is simple in process, and easy for process control; moreover, the various materials and reagents are all common in the market, so that the production cost is low; the alpha-arbutin produced by the fermentation method provided by the invention is high in conversion rate and capable of being greatly subjected to industrial production.

Description

technical field [0001] The invention belongs to the field of biological fermentation, and in particular relates to a method for producing α-arbutin by biological fermentation. Background technique [0002] Arbutin, also known as arbutin, arbutin, arbutin, arbutin or myricetin, is a kind of perennial evergreen shrub plant - bearberry, which is derived from the leaf cells of the Rhododendronaceae bearberry skin depigmenting ingredients. [0003] Arbutin is a natural active substance derived from green plants. It is a skin depigmentation component that integrates "green plants, safe and reliable" and "high-efficiency decolorization". It can quickly penetrate into the skin without affecting cell proliferation. At the same time, it can effectively inhibit the activity of tyrosinase in the skin, block the formation of melanin, and accelerate the decomposition and excretion of melanin by directly combining itself with tyrosinase, thereby reducing skin pigmentation, removing spots ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/44C12R1/69
Inventor 刘微波刘娟樊媛媛华寿虎
Owner FENCHEM BIOTEK
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