Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bacterial engineering

一种细菌、突变体的技术，应用在生物技术应用领域，能够解决方式不完全理解、不能等问题

Inactive Publication Date: 2015-09-09
BACTEVO
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This approach is currently impractical because gene products and regulatory elements coordinate and crosstalk throughout the cellular environment in ways that are not currently fully understood and thus cannot be addressed as formal modules

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacterial engineering
  • Bacterial engineering
  • Bacterial engineering

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0118] Example 1: Production of mutant bacteria exhibiting improved survival and / or growth in the presence of fosfomycin

[0119] (i) Activated transposon (Tn A ) construction

[0120] The constructed plasmid incorporates an amplifiable nucleotide sequence that acts as a transposon. Elements of the transposon include a 19 bp mosaic end recognized by a specific transposase and define a transposon (an antibiotic for selection of transformants produced by transposition- resistance gene), and an outward oriented promoter at one end of the transposon to activate the expression of a target gene adjacent to the transposon insertion site.

[0121] Alternative plasmids were constructed from different genes from E. coli, Acinetobacter, Pseudomonas or Rhodopseudomonas using different outwardly directed promoters. Table 1 provides details of the different promoters used. In addition, different host species bacteria require different antibiotic resistance genes to select transformants,...

Embodiment 2

[0157] Example 2: Mutant Rhodopseudomonas palustris exhibiting improved growth in industrial waste production

[0158] Rhodopseudomonas palustris is a purple photosynthetic bacterium that belongs to the α-proteobacteria. It has extraordinary metabolic versatility, growing by any of four life-supporting metabolic modes: photoautotrophy and photosynthesis (energy from light and carbon from carbon dioxide), photoheterotrophy (energy from light and carbon from organic compounds), chemoheterotrophy (carbon and energy from organic compounds) and chemoautotrophy (energy from inorganic compounds and carbon from carbon dioxide). This metabolic flexibility facilitates the use of this bacterium in biotechnological applications, including bioremediation of industrial waste.

[0159] Preparation of Electrocompetent Rhodopseudomonas palustris

[0160] Electrocompetent cells for transposon mutagenesis were prepared by inoculating 500 mL of Yeast Peptone Dextrose (YPD) broth with Rhod...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Described is a process for producing a mutant bacterium which exhibits improved survival and / or growth under a selected growth condition, the process comprising the steps of: (a) generating a pool of mutant bacteria by transposon mutagenesis with an activating transposon (TnA), wherein the TnA comprises a promoter capable of increasing transcription of a gene at or near its insertion site; (b) growing bacteria from the mutant pool under the selected growth condition and under one or more reference conditions to produce two or more test cultures; and (c) comparing the distribution of TnA insertions between test cultures to identify a first class of genes which are disadvantageous for growth and / or survival under the selected growth condition and a second class of genes which are advantageous for growth and / or survival under the selected growth condition.

Description

technical field [0001] The present invention relates to design (engineering, engineering) suitable for biotechnology applications, including the production of proteins, secondary metabolites (secondary metabolites) and biofuels, biocatalysis, bioremediation (bioremediation), biotransformation, biodegradation , methods for biological control, drug development, drug screening, vaccines, probiotics, bacterial cells for biosensors and drug delivery vehicles. Background technique [0002] Bacteria find applications in many aspects of biotechnology, including as hosts for the production of heterologous proteins and peptides (including enzymes and therapeutic antibodies), as sources of secondary metabolites or their derivatives, as biological controls, biological Reagents for degradation, biofuel production, biocatalysis and bioremediation and as probiotics, vaccine components and drug delivery systems. [0003] Natural bacterial strains are not optimized for biotechnological use....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
CPCC12N15/102C12N15/1082C12N15/1058
Inventor 戴维·休·威廉斯阿瑟·基思·特纳约翰·理查德·韦恩
Owner BACTEVO
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com