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Method for extracting polysaccharide and catalpol in dried rehmannia root

A technology of rehmannia glutinosa and polysaccharides, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of high cost, cumbersome process, low yield of polysaccharides and catalpol, and achieve low implementation cost and extraction Simple method and high extraction efficiency

Inactive Publication Date: 2015-09-09
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved in the present invention is to provide a method for simultaneously extracting polysaccharides and catalpol from Rehmannia glutinosa, which can solve the problems of low yield, cumbersome process, and high cost of extracting polysaccharides and catalpol in the prior art, and obtain polysaccharides and catalpol. The yield of polysaccharide and catalpol in the alcohol mixture is high, it has the advantages of simple and safe process, high extraction efficiency, low cost, high economic efficiency, is conducive to environmental protection, and is suitable for industrial production

Method used

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  • Method for extracting polysaccharide and catalpol in dried rehmannia root
  • Method for extracting polysaccharide and catalpol in dried rehmannia root
  • Method for extracting polysaccharide and catalpol in dried rehmannia root

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Experimental program
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Effect test

preparation example Construction

[0025] (2) preparation of need testing solution

[0026] Precisely weigh about 10 mg of the sample in a 50 ml volumetric flask, distill the volume to the mark with distilled water, and after fully dissolving, take 0.1 ml into a stoppered test tube, add water to 2 ml, add 1 ml of 5% phenol test solution, shake well, and quickly add concentrated Sulfuric acid 5ml, mixed quickly, let stand for 5min, heated in boiling water bath for 15min, cooled to room temperature, then the test sample was obtained, measured absorbance, calculated polysaccharide content, recorded as α (%).

[0027] 2 Determination method of catalpol content

[0028] (1) Chromatographic conditions

[0029] Column: XBridge TM C18 (5μm 4.6x250mm); column temperature 30°C; detection wavelength 210nm; mobile phase: acetonitrile-water (1:99); injection volume 20μl; flow rate 1ml / min.

[0030] (2) Preparation of standard curve

[0031] Weigh 10mg of catalpol reference substance, place it in a 100mL volumetric flask...

Embodiment 1

[0076] Extraction of Rehmannia glutinosa

[0077] Take Rehmannia glutinosa, dry it to constant weight, crush it, pass through a 20-mesh sieve, take 100.00g of mass, add 800ml of distilled water, and extract 1.5h each time at 90°C, extract twice, combine the extracts, and concentrate to a density of 1.10g / ml to obtain 280ml of concentrated solution.

[0078] spray drying

[0079] According to 280ml of concentrated solution, the density is 1.10g / ml, the calculated amount of 5% maltodextrin needs to be added is 16.21g. Add maltodextrin into the concentrated solution, mix well, and after fully dissolving, select the inlet air temperature of 150°C and the outlet air temperature of about 80°C, and spray dry to obtain the product with a mass of 83.19g.

[0080] Total sugar and catalpol content

[0081] According to the determination method of the aforementioned rehmannia polysaccharide and catalpol, the total sugar content is 84.07%, and the catalpol content is 1.00%.

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Abstract

The invention relates to the technical field of extraction of active substances in Chinese medicinal herbs and particularly discloses a method for extracting polysaccharide and catalpol in dried rehmannia root. The method includes the following steps: firstly, performing sample pretreatment, and drying the dried rehmannia root to constant weight prior to smashing and sieving; secondly, for extraction, uniformly mixing the treated dried rehmannia root and distilled water with the solid-to-liquid ratio being 1:(8-12)g / ml, heating to the temperature of 90-100 DEG C, and extracting for 1-2 hours for two times to three times, and combining extracting solution; thirdly, concentrating the extracting solution and drying to obtain finished products. Further, in the third step, the extracting solution is concentrated to a specific weight of 1.10g / ml and is added with 5% of maltodextrin, inlet air temperature of a spray dryer is 150+ / -2 DEG C, and outlet air temperature is 80+ / -2 DEG C. Yield of the polysaccharide and catalpol in prepared polysaccharide and catalpol mixture is high, and the method has the advantages of simple techniques, less extraction time and low cost and is suitable for industrial production.

Description

technical field [0001] The invention relates to the technical field of extraction of effective substances in Chinese medicinal plants. Background technique [0002] Rehmannia glutinosa, also known as Rehmannia glutinosa, Shengdi, Dimai, etc., is one of the commonly used tonic traditional Chinese medicines. It is widely used in clinical practice, and its active ingredients are mainly polysaccharides and catalpol. Polysaccharides are compounds composed of many identical or different monosaccharides with α- or β-glycosidic bonds. They are commonly found in plants in nature, including starch, cellulose, polysaccharides, pectin, etc. Due to the wide range of sources of plant polysaccharides, the molecular composition and molecular weight of different plant polysaccharides are different. Relevant studies have pointed out that rehmannia polysaccharides have activities such as improving immunity, inhibiting tumors, anti-aging, promoting hematopoietic function, and lowering blood su...

Claims

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Application Information

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IPC IPC(8): C08B37/00C07H17/04C07H1/08
Inventor 刘聚祥郑桦
Owner HEBEI AGRICULTURAL UNIV.
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