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Microalbuminuria detection kit and preparation thereof

A urine microalbumin and detection kit technology, applied in the biological field, can solve the problems of many operation steps, poor repeatability, radioactive contamination, etc.

Inactive Publication Date: 2015-08-19
王贤俊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Radioimmunoassay has high sensitivity and strong specificity, but has the disadvantages of radioactive contamination and short shelf life of reagents.
At present, the most widely used detection method for urine microalbumin in China is still the ELISA method, but the enzyme-labeled method has many steps, so the repeatability is poor

Method used

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  • Microalbuminuria detection kit and preparation thereof
  • Microalbuminuria detection kit and preparation thereof
  • Microalbuminuria detection kit and preparation thereof

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Experimental program
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Effect test

Embodiment

[0036] The performance index comparison between the reagent of the present invention and the commercially available reagent A:

[0037] 1) Precision measurement: The same sample is drawn for 20 consecutive times for measurement, and the mean, standard deviation and coefficient of variation of the measured values ​​are calculated. CV = SD X ‾ × 100 %

[0038] Table 1 Precision test results

[0039]

[0040]

[0041] The coefficient of variation CV is usually used to measure the precision of a measurement method. The smaller the CV value, the better the precision of the measurement method. For clinical chemistry testing items, a method precision with a CV of less than 5% is generally accepted as acceptable. The CV value of the reagent of the present invention in Table 1 is lower than that of the commercially available reagent A, indicating that the precision of the method of the present invention is better than that of the commercially available reagent A. ...

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Abstract

The invention provides a microalbuminuria (mAlb) detection kit, which is based on a latex enhanced turbidimetric immunoassay method, is a liquid double reagent, namely reagent R1 and reagent R2, wherein the reagent R1 is a reactant and the reagent R2 is a solution containing albumin immuno latex particles, and the detection kit is characterized by crosslinking by applying a chemical process, and covalently crosslinking a goat anti-human albumin antibody (also called goat anti-human mAlb antibody) with carboxylated polystyrene latex through water-soluble carbodiimide (EDC) and H-hydroxy succinimide (NHS), so as to form an mAlb antibody latex reagent. The reagent has the advantages of high sensitivity, strong specificity and simple preparation, and is worthy of further promotion.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and specifically relates to a urine microalbumin (mAlb) detection kit and preparation thereof. Background technique: [0002] Microalbuminuria refers to the appearance of microalbumin in the urine. Albumin is a normal protein in the blood, but only a very small amount of albumin appears in the urine under physiological conditions. Microalbuminuria reflects abnormal protein leakage from the kidneys. The increase in urine microalbumin is more common in diabetic nephropathy, hypertension, preeclampsia, and is an early sensitive indicator of kidney injury. No matter what kind of disease, the urine microalbumin is caused by the damage of the inherent cells of the kidney due to different initial reasons, which changes the structure of the inherent cells of the kidney, and the function changes with the changes in the structure, which is reflected in the urine. When the urine microalbumin is found to be in th...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/347
Inventor 王贤俊郑蓓蕾郭二豪江新涛
Owner 王贤俊
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