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Preparation method and application of a photoelectrochemical sensor based on ag2secdse-labeled sandwich cardiac troponin T

A cardiac troponin and photoelectrochemical technology, which is applied in the field of photoelectrochemical sensor preparation, can solve the problems of low photoelectric conversion efficiency and difficult application, and achieve the effects of excellent photoelectric conversion characteristics, high sensitivity detection, and simple operation.

Inactive Publication Date: 2016-04-20
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] TiO 2 It is a semiconductor material widely used in photocatalysis, solar cells and fuel cells. However, due to TiO 2 With a wide band gap (3.2eV), it can only be effectively excited in the ultraviolet region, so the photoelectric conversion efficiency in the visible region is extremely low, and TiO 2 Nanoribbons have high electron-hole recombination efficiency, which brings certain difficulties to their application.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 An Ag-based 2 The preparation method of the photoelectrochemical sensor of the sandwich-type cardiac troponin T labeled with SeCdSe:

[0029] (1) Pretreatment of ITO electrodes: Cut the ITO conductive glass to a size of 2cm×0.5cm, ultrasonically clean it with detergent, acetone, ethanol and ultrapure water for 30 minutes, and dry it with nitrogen.

[0030] (2) Add 6μL, 4mg / mL CeO dropwise2 -TiO 2 The solution was placed on the surface of the electrode and dried at room temperature.

[0031] (3) Add 6 μL, 8 μg / mL cardiac troponin T capture antibody Ab 1 On the surface of the electrode, dry it in a refrigerator at 4°C, and rinse it with ultrapure water;

[0032] (4) Drop 3 μL of 0.5% BSA solution on the surface of the electrode to seal the non-specific active sites on the surface of the electrode, and dry it in a refrigerator at 4°C;

[0033] (5) Wash with ultrapure water, dry to a wet state, apply 6 μL of cardiac troponin T antigen solution with different...

Embodiment 2

[0035] Example 2 An Ag-based 2 The preparation method of the photoelectrochemical sensor of the sandwich-type cardiac troponin T labeled with SeCdSe:

[0036] (1) Pretreatment of ITO electrodes: Cut the ITO conductive glass to a size of 2cm×0.5cm, ultrasonically clean it with detergent, acetone, ethanol and ultrapure water for 30 minutes, and dry it with nitrogen.

[0037] (2) Add 6 μL, 5 mg / mL CeO dropwise 2 -TiO 2 The solution was placed on the surface of the electrode and dried at room temperature.

[0038] (3) Add 6 μL, 10 μg / mL cardiac troponin T capture antibody Ab 1 On the surface of the electrode, dry it in a refrigerator at 4°C, and rinse it with ultrapure water;

[0039] (4) Drop 3 μL of BSA solution with a mass fraction of 1% on the surface of the electrode to seal the non-specific active sites on the surface of the electrode, and dry it in a refrigerator at 4°C;

[0040] (5) Wash with ultrapure water, dry to a wet state, apply 6 μL of cardiac troponin T anti...

Embodiment 3

[0042] Example 3 An Ag-based 2 The preparation method of the photoelectrochemical sensor of the sandwich-type cardiac troponin T labeled with SeCdSe:

[0043] (1) Pretreatment of ITO electrodes: Cut the ITO conductive glass to a size of 2cm×0.5cm, ultrasonically clean it with detergent, acetone, ethanol and ultrapure water for 30 minutes, and dry it with nitrogen.

[0044] (2) Add 6μL, 6mg / mL CeO dropwise 2 -TiO 2 The solution was placed on the surface of the electrode and dried at room temperature.

[0045] (3) Add 6 μL, 12 μg / mL cardiac troponin T capture antibody Ab 1 On the surface of the electrode, dry it in a refrigerator at 4°C, and rinse it with ultrapure water;

[0046] (4) Drop 3 μL of BSA solution with a mass fraction of 2.0% on the surface of the electrode to seal the non-specific active sites on the surface of the electrode, and dry it in a refrigerator at 4°C;

[0047] (5) Wash with ultrapure water, dry to a wet state, apply 6 μL of cardiac troponin T anti...

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Abstract

The invention relates to a preparation method and application of a photoelectrochemical sensor based on Ag2SeCdSe-labeled sandwich cardiac troponin T, and belongs to the technical fields of new functional materials and biosensing detection. Specifically, the present invention uses CeO2-TiO2 composite nanomaterials with photocatalytic activity as the photoactive base material, uses Ag2SeCdSe as the antibody marker, and prepares a sandwich-type photoelectrochemical sensor through the signal amplification effect of the marker Ag2SeCdSe on the base material, which is used for myocardial Highly sensitive detection of the injury-specific marker cardiac troponin T. This method is of great significance to the early diagnosis and treatment of acute myocardial infarction.

Description

technical field [0001] The present invention relates to an Ag-based 2 Preparation method and application of SeCdSe-labeled sandwich cardiac troponin T photoelectrochemical sensor. specifically CeO 2 -TiO 2 Composite nanomaterials as photoactive substrate materials, with Ag 2 SeCdSe is an antibody label, through the label Ag 2 The invention relates to the signal amplification effect of SeCdSe on a base material, and prepares a sandwich-type photoelectrochemical sensor for detecting cardiac troponin T, which belongs to the technical field of new functional materials and biosensing detection. Background technique [0002] Photoelectrochemical detection is an emerging bioanalytical detection method. In the photoelectrochemical detection process, light is the excitation signal, and the electrical signal is the detection signal. The background signal is reduced due to the complete separation of the excitation signal and the detection signal, so that photoelectrochemistry has ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/327G01N33/68G01N33/543G01N33/58
Inventor 柳懿芯魏琴马洪敏范大伟王晓东胡丽华杜斌曹伟李贺吴丹
Owner UNIV OF JINAN
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