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Method for extracting longan seed polyphenol

A technology of longan core and reflux extraction, which is applied in the fields of pharmaceutical formula, food science, plant raw materials, etc., to achieve the effects of low production cost, high extraction efficiency and convenient operation

Inactive Publication Date: 2015-08-19
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the antibacterial activity of polyphenols in plant foods and medicinal plants against different microorganisms has also been extensively studied. In addition, polyphenols also have biological activities such as preventing hypertension, antithrombotic, antigastric ulcer, antiviral, and skin protection. The results of a large number of experimental studies on health care and beauty functions show that plant polyphenols have antioxidant, anti-cancer, anti-aging, and antibacterial effects. Adequate intake of plant polyphenols can prevent and inhibit the occurrence of diseases. The potential of oxidizing agent and food, health products, and cosmetic active raw materials has huge development value and broad market prospect. However, at the present stage, there is no process method with practical production significance for the extraction and purification process of longan kernel polyphenols. to report

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A method for extracting longan kernel polyphenols, comprising the following steps:

[0017] 1), peeling and crushing: select the longan kernels without pests and wash them, bake them at a temperature of 30°C for 25 hours, peel them, and then put them into a pulverizer to pulverize them to obtain longan kernel powder. The particle size of the longan kernel powder is 15 mesh ;

[0018] 2), Filtration: Add an ethanol solution of 4 times the weight of the longan kernel powder to the longan kernel powder in step 1), reflux extraction at 50° C. for 4 hours, filter the obtained extract, and collect the filtrate;

[0019] 3), extraction: adding the filtrate obtained in step 2) to ethyl acetate for extraction, collecting the ethyl acetate part, and performing silica gel column chromatography on the ethyl acetate extraction part, polyphenols and silica gel are adsorbed, and non-polyphenol components are removed ; Then adopt sherwood oil-chloroform gradient elution, collect eluat...

Embodiment 2

[0023] A method for extracting longan kernel polyphenols, comprising the following steps:

[0024] 1), peeling and crushing: select longan kernels without pests, wash them, bake them at 60°C for 27 hours, peel them, and then put them into a grinder to grind them to obtain longan kernel powder. The particle size of longan kernel powder is 20 mesh ;

[0025] 2), Filtration: Add an ethanol solution of 6 times the weight of the longan kernel powder to the longan kernel powder in step 1), reflux extraction at 60° C. for 5 hours, filter the obtained extract, and collect the filtrate;

[0026] 3), extraction: adding the filtrate obtained in step 2) to ethyl acetate for extraction, collecting the ethyl acetate part, and performing silica gel column chromatography on the ethyl acetate extraction part, polyphenols and silica gel are adsorbed, and non-polyphenol components are removed ; Then adopt sherwood oil-chloroform gradient elution, collect eluate;

[0027] 4) Concentration and a...

Embodiment 3

[0030] A method for extracting longan kernel polyphenols, comprising the following steps:

[0031] 1), peeling and crushing: select longan kernels without pests and wash them, bake them at a temperature of 70°C for 30 hours, peel them, and then put them into a pulverizer to grind them to obtain longan kernel powder. The particle size of longan kernel powder is 20 mesh ;

[0032] 2), Filtration: Add an ethanol solution of 8 times the weight of the longan kernel powder to the longan kernel powder in step 1), reflux extraction at 80° C. for 7 hours, filter the obtained extract, and collect the filtrate;

[0033] 3), extraction: adding the filtrate obtained in step 2) to ethyl acetate for extraction, collecting the ethyl acetate part, and performing silica gel column chromatography on the ethyl acetate extraction part, polyphenols and silica gel are adsorbed, and non-polyphenol components are removed ; Then adopt sherwood oil-chloroform gradient elution, collect eluate;

[0034]...

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PUM

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Abstract

The invention discloses a method for extracting longan seed polyphenol, and belongs to the field of natural product extraction method. The method comprises the following steps: 1) peeling and crushing; 2) filtering; 3) extracting; and 4) concentrating to obtain the polyphenol. The invention has the advantages of reasonable control of drying level of the longan seed, reduction of activity of polyphenol oxidase in longan seed, reduction of the loss of polyphenol in the production process, easiness to operate, high extraction efficiency and low production cost. The method has important meaning for expanding the use of longan seed and increasing their added value, not only is conducive to the full use of longan resources, but also helps promoting the development of longan planting.

Description

technical field [0001] The invention belongs to the field of extraction methods of natural products, in particular to a method for extracting polyphenols from longan seeds. Background technique [0002] Longan, the fruit of Sapindaceae plant longan, is warm in nature and sweet in taste, nourishes the heart and spleen, nourishes qi and blood, and has a good nourishing and nourishing effect. It can be used for insomnia, forgetfulness, palpitations, dizziness and other diseases caused by deficiency of heart and spleen, deficiency of qi and blood. China It is a big longan producing country in the world. It is mainly produced in Guangdong, Guangxi, Fujian, Sichuan and other places. The planting area exceeds 10,000 tons, and the annual output value is nearly 100 million yuan, ranking first in the world. Through comprehensive utilization, the discarded longan cores weigh hundreds of thousands of tons every year. [0003] Longan kernels are rich in natural products such as starch, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/77A61K8/97A23L1/30A61K131/00
Inventor 蓝擎
Owner GUANGXI UNIV
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