A kind of ganoderma lucidum crabapple tea and its preparation process and application
A preparation process and technology of crabapple tea, which can be applied to tea substitutes, medical preparations containing active ingredients, plant raw materials, etc., can solve problems such as unreported health care functions, and achieve considerable application, easy availability of materials, and good synergy. Effect
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Embodiment 1
[0026] Take Ganoderma lucidum fruiting body slices, and take Chinese crabapple tea leaves, and dry them in an oven preheated to 60-80°C for 20-40 minutes, control the water content below 8%, crush the dried Ganoderma lucidum fruiting body slices and dried Chinese crabapple tea leaves respectively, And pass through 20-60 mesh; by weight, mix 10-200 parts of ganoderma lucidum with 80-120 parts of crabapple, crush and sieve again, pass through 80-120 mesh, and pack in 0.2-5g per bag.
[0027] Ganoderma lucidum and crabapple water extracts on CCL 4 Synergistic protection from induction of hepatocellular injury:
[0028] Take 1 Kunming mouse about 10g, decapitate it, separate the liver aseptically, put it in PBS solution after weighing, perfuse and wash the liver until it is grayish white, cut the liver into tissue pieces of about 1mm3, grind it with a nylon mesh, add Centrifuge the PBS solution at 1000r / min for 5min, remove the supernatant, add 10ml of culture medium, and blow ge...
Embodiment 2
[0041] Take Ganoderma lucidum spore powder and break the wall, and take another Chinese crabapple tea and dry them in an oven preheated to 60-80°C for 20-40 minutes, control the water content below 8%, respectively crush the dried Ganoderma lucidum spore powder and dried Chinese crabapple tea leaves, And pass through 20-60 mesh; by weight, mix 10-200 parts of Ganoderma lucidum spore powder with 80-120 parts of crabapple, crush and sieve again, pass through 80-120 mesh, and pack with 0.2-5g per bag .
[0042] Synergistic protective effects of Ganoderma lucidum and Begonia extracts on mice with chronic alcoholic liver injury:
[0043]Male Kunming mice were randomly divided into groups, 10 mice in each group, administered orally, and the normal control group and model group were given corresponding volumes of water. Except for the normal control group, 38° liquor (0.1ml / 10g) was intragastrically administered every morning for 40 consecutive days. After fasting for 16 hours on t...
Embodiment 3
[0049] Make potato culture medium: 200g potatoes, 20g glucose, 20g agar, 1000ml water, cut potatoes into small pieces and put them in a pot, add 1000ml water, boil for 20 minutes, filter with gauze, add water to 1000ml, add agar and glucose, add After warming and dissolving, adjust the pH to 6.0 with potassium dihydrogen phosphate or dipotassium hydrogen phosphate solution, divide the culture medium into Erlenmeyer flasks, fill each bottle with 1 / 3 capacity culture medium, and sterilize with steam at 0.1-0.15 MPa for 30 minutes;
[0050] Strain activation: take out the ganoderma lucidum strain from the strain bank, inoculate it on a sterile operating table, use a shaker for shaking culture, the rotation speed is 150r / min, the temperature is 25°C, and cultivate for 5-7 days;
[0051] Make seed culture solution: glucose 20g, gypsum 2g, soybean powder 10g, malt powder 2g, 20% potato juice 1000ml after preparation, adjust the pH to 6.0 with potassium dihydrogen phosphate or dipotas...
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