Polar body genome reconstruction ovum, preparation method and application thereof

Abstract

The invention belongs to the bio-medical field, relates to a polar body genome and an application thereof, and particularly relates to a polar body genome reconstruction ovum, a preparation method and an application thereof. In the invention, according to a characteristic that a polar body not only contains female DNA being same as that in egg cell cytoplasm but also carries little even no cytoplasm substances (mitochondria), the polar body genome reconstruction ovum and an application thereof in preparation of a material for preventing mitochondria maternal genetic diseases are provided in the invention. An animal model test proves that the reconstruction ovum and progenies thereof are free of mitochondria DNA of patients, so that the reconstruction ovum can effectively stop cytoplasm-related diseases and can actively and completely prevent the mitochondria maternal genetic diseases.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a polar body genome and its use, in particular to a polar body genome restructured egg and its preparation method and use, especially its use in preparing materials for treating maternal mitochondrial genetic diseases. Background technique [0002] According to research reports, mitochondrial DNA (mitochondrial DNA, mtDNA) is another genome independent of the nucleus extrachromosomal, known as human chromosome 25, and its genetic characteristics are non-Mendelian inheritance, also known as maternal inheritance ), that is, the mother passes on mtDNA to her sons and daughters, but only the daughter can pass on her mtDNA to the next generation. Mitochondrial disease (mitochondriopathy) refers to a group of multi-system diseases caused by defects in mitochondrial metabolic enzymes caused by genetic defects, resulting in ATP synthesis disorders and insufficient energy sources. Among them, mt...

AI Technical Summary

Problems solved by technology

However, research practice has shown that both metaphase chromosomes and prokaryotes are surrounded by a large number of mitochondria due to energy requirements, accounting for about 40% of the total mitochondria in the cytoplasm. During the process of nuclear replacement, these mitochondria The nucleus enters the new egg cytoplasm, so that the reconstructed egg after replacement still contains a certain amount of mutant mtDNA, which may pass through the "genetic bottleneck" and replicate and amplify in the offspring, accumulating to a certain threshold. still cause disease in offspring

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