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c, d type clostridium perfringens antitoxin serum and preparation method thereof

A technology of Clostridium perfringens and perfringens, applied in the direction of serum immunoglobulin, anti-bacterial immunoglobulin, etc., can solve the problems of no preparation and application, achieve good prevention and control effect, reduce losses, high potency effect

Active Publication Date: 2018-03-27
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In my country, there is no report on preparation and application of Clostridium perfringens type C and D antitoxin serum

Method used

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  • c, d type clostridium perfringens antitoxin serum and preparation method thereof
  • c, d type clostridium perfringens antitoxin serum and preparation method thereof
  • c, d type clostridium perfringens antitoxin serum and preparation method thereof

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Embodiment 1

[0021] The detailed process of embodiment 1 serum preparation

[0022] First, prepare the bacterial liquid. Clostridium perfringens type C (National Collection of Type Cultures-British Microbial Culture Collection Center preservation number: NCTC 3180) and Clostridium perfringens type D (National Collection of Type Cultures-British microbial strains) Preservation Center Preservation No.: NCTC8346) were respectively inoculated on the blood plate culture medium for 37°C and 36h recovery, respectively picked a colony and cultured in the nutrient broth, and the gas concentration was 88%N 2 , 7%H 2 , 5%CO 2 Cultivate at 39°C for 13 hours under the anaerobic environment conditions to obtain Clostridium perfringens type C and Clostridium perfringens type D respectively, which were reserved as inoculation medium for toxin production.

[0023] Next, prepare the immunogen. Add 4ml of Clostridium perfringens type C and 3ml Clostridium perfringens type D to 100ml pH7.5 toxin-producing...

Embodiment 2

[0028] Example 2. Verification of vaccine safety and immune effect.

[0029] Safety inspection: get 5 healthy mice of 16~20g, intravenously inject 0.5ml of the antiserum prepared by the present invention respectively; simultaneously use 2 guinea pigs with a body weight of 250g-450g, subcutaneously inject 5ml of the antiserum prepared by the present invention, and observe continuously for 10 days ; There were no local adverse reactions such as granuloma, and no abnormal reactions in the whole body. (Refer to "Quality Standards of Veterinary Biological Products of the People's Republic of China")

[0030]The mensuration of absolute lethal dose: the C type and D type Clostridium perfringens exotoxin solution prepared by the present invention are respectively diluted with physiological saline 5, 10, 15, 20, 30, 40, 50, 60 times respectively to 8 Group mice were intraperitoneally injected with 0.5ml, 5 in every group, and the toxin content of the maximum dilution multiple (60 bein...

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Abstract

The invention relates to a type C and D type Clostridium perfringens antitoxin serum and a preparation method thereof; the C type Clostridium perfringens strain and the D type Clostridium perfringens strain are respectively inoculated on a blood plate Resuscitate on the medium, and then use the nutrient broth to enrich the bacteria for anaerobic culture to obtain the C-type Clostridium perfringens liquid and the D-type Clostridium perfringens liquid respectively, and then use the toxin-producing medium to cultivate and inactivate. It is isolated from serum after basic immunization and booster immunization. The antitoxin serum of C and D type Clostridium perfringens prepared by the present invention has high titer, strong specificity and no side effects; it can be used for the immunity of C and D types of Clostridium perfringens in animals, and can be used in research Pre-control plays an important role in the epidemic of sheep sudden attack and enterotoxemia caused by type C, D or C and D two types of Clostridium perfringens infection.

Description

(1) Technical field [0001] The invention relates to a type C and D type Clostridium perfringens antitoxin serum and a preparation method thereof. (2) Technical background [0002] Clostridium perfringens (C.perfringens), also known as Clostridium welchii (C.welchii), is a common bacteria in the intestinal tract of animals. opportunistic pathogenic bacteria. The bacteria can infect a variety of animals, leading to complex symptoms or pathological changes such as necrotic enteritis and enterotoxemia. Clostridium perfringens is divided into five subtypes A, B, C, D, and E according to the four main exotoxins (α, β, ε, ι) secreted by it. The main virulence-causing toxins of Clostridium perfringens type C are mainly α and β exotoxins, which are the main pathogens of a variety of livestock enterogenous toxemia and "sudden death syndrome", such as red scour in piglets and sudden death in sheep. Clostridium perfringens type D mainly produces α and ε exotoxins, which can cause ent...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/12C07K16/06
Inventor 柴同杰刘雪慧王海荣
Owner SHANDONG AGRICULTURAL UNIVERSITY
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