A kind of artificial domestication and cultivation method of wild ring peduncle mushroom
An artificial technology of ring stalk mushroom, which is applied in mushroom cultivation, cultivation, plant cultivation, etc., can solve the problems of artificial domestication and cultivation of wild ring stalk mushroom, and achieve the effect of wide application value and good mycelium growth
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Embodiment 1
[0038] Embodiment one: the method for artificial domestication and cultivation of wild ring peduncle
[0039] A method for the artificial domestication and cultivation of wild ring peduncle, the specific steps of the method are as follows:
[0040] (1) Isolation of fruiting body tissue of wild Puccinia ringscens: Split the fruiting bodies of wild Ringing mushrooms longitudinally along the cap and stipe in a sterile microenvironment, and then use sterilized tweezers to quickly separate the fruiting body cap and Take soybean grain-sized sporocarp meat tissue from the junction of the stipe and inoculate it on the slant of conventional PDA or PSA mother seed medium, and culture it in the dark at a constant temperature of 25°C for 7d-10d; Pick the tip mycelium, transplant it to the slant of conventional PDA or PSA medium, and culture it in the dark at a constant temperature of 25°C until the mycelium covers the entire slant, and repeat this 3 times.
[0041] (2) Propagation of the...
Embodiment 2
[0052] Embodiment two: the method for artificial domestication and cultivation of wild ring peduncle
[0053] A method for the artificial domestication and cultivation of wild ring peduncle, the specific steps of the method are as follows:
[0054] (1) Isolation of fruiting body tissue of wild Puccinia ringscens: Split the fruiting bodies of wild Ringing mushrooms longitudinally along the cap and stipe in a sterile microenvironment, and then use sterilized tweezers to quickly separate the fruiting body cap and Take soybean grain-sized sporocarp meat tissue from the junction of the stipe and inoculate it on the slant of conventional PDA medium, culture at a constant temperature of 25°C in the dark for 7d-10d; The filaments were transplanted to the slant of conventional PDA medium, and cultured in the dark at a constant temperature of 25°C until the mycelium covered the entire slant, and this was repeated 3 times.
[0055] (2) Propagation of the first-level species: transfer th...
Embodiment 3
[0066] Example Three: Mushroom Fruiting Management
[0067] Suitable conditions required during the fruiting period: temperature 20°C-28°C, relative air humidity 85%-95%, compost moisture content 65%, covering soil moisture content 22%, and fresh air.
[0068] 1. Pre-production management:
[0069] (1) Moisture management: master the principle of spraying water on mushrooms, spray more water when there are many mushrooms, if the humidity of the bed surface is enough, spray once every 1d-2d, so as not to spray too much water and cause mycelia to suffocate.
[0070] (2) Ventilation management: The ventilation time depends on the air quality in the mushroom shed. Ventilate more when spraying water and after spraying water to avoid dampness and stuffiness in the mushroom shed, which will cause mycelium to die.
[0071] (3) Topdressing: Due to the fast mushrooming in the early stage of mushroom production and a large number of mushrooms, timely and appropriate topdressing has a s...
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