An antimicrobial peptide pjf11 and its application in inhibiting peri-implantitis bacteria

A technology of PJF11 and antimicrobial peptides, applied in the fields of application, chemicals for biological control, peptides, etc., can solve the problems of difficult synthesis, high synthesis cost, long peptide chain, etc., achieve low hemolysis, low production cost, The effect of short peptide chains

Active Publication Date: 2019-01-15
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

SMAP29, LL-37, HBD2, HBD3, Cecropin B, etc. reported in the existing literature all show strong antibacterial effects, but at the same time, these antibacterial peptides have different peptide chain lengths, difficulty in synthesis, and high cost of synthesis. degree of cytotoxicity

Method used

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  • An antimicrobial peptide pjf11 and its application in inhibiting peri-implantitis bacteria
  • An antimicrobial peptide pjf11 and its application in inhibiting peri-implantitis bacteria
  • An antimicrobial peptide pjf11 and its application in inhibiting peri-implantitis bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The inventors designed an antimicrobial peptide PJF11, the amino acid sequence of which is shown in SEQ ID NO: 1, and one end of the peptide chain is connected with an acetate group, and the other end is connected with an amino group. The antimicrobial peptide PJF11 was synthesized by Zhejiang Ontores Biotechnologies Company.

[0029] This example studies the minimum inhibitory concentration (MIC) of the antimicrobial peptide PJF11 on peri-implantitis bacteria Fusobacterium nucleatum and Staphylococcus aureus.

[0030] Experimental method: Take the bacterial suspension in the logarithmic growth phase and adjust the bacterial concentration to about 1×10 6 cfu / ml, take the antibacterial peptide PJF11 dry powder and dilute it with the corresponding broth to prepare a series of concentrations of 400.0, 200.0, 100.0, 50.0, 25.0, 12.5, 6.2 μg / ml with a 2-fold decreasing concentration. After mixing the bacterial suspension, a series of final concentrations of 200.0, 100.0, 50...

Embodiment 2

[0034] In this example, the minimum bactericidal concentration (MBC) of the antimicrobial peptide PJF11 on peri-implantitis bacteria Fusobacterium nucleatum and Staphylococcus aureus was studied.

[0035]Experimental method: take the minimum inhibitory concentration (25 μg / ml for both bacteria) measured in the micro broth dilution experiment of Example 1 as the MIC group, select the MIC group (25 μg / ml) and the concentration higher than the MIC group (200.0 , 100.0, 50.0 μg / ml) respectively pipette 50 μl of liquid corresponding to each well, and evenly spread it on the plate medium with an L-shaped coating rod. After Fusobacterium nucleatum was observed in an anaerobic incubator for 24 to 48 hours, and after Staphylococcus aureus was observed in a 37°C constant temperature incubator for 16 to 24 hours, the lowest peptide concentration corresponding to the plate with no colony growth was used as the minimum bactericidal concentration , the above experiment was repeated 3 times....

Embodiment 3

[0038] In this example, the antibacterial curve of the antimicrobial peptide PJF11 against Staphylococcus aureus was determined.

[0039] Test method: Take the bacterial liquid in the logarithmic growth phase, adjust the bacterial concentration to about 1×10 6 cfu / ml. Take out the LB broth medium from the refrigerator and place it in a constant temperature incubator for 10 minutes. Drug groups (MIC concentration group and 0.5MIC concentration group) were set up, in which the MIC concentration group was bacterial suspension with a final concentration of antimicrobial peptide PJF11 of 25 μg / ml, and the 0.5MIC concentration group was bacterial suspension with a final concentration of antimicrobial peptide PJF11 of 12.5 μg / ml. Suspension, and a negative control group, which is the bacterial suspension without any drug. In the drug group, 100 μl of drug solution and bacterial solution were added to each well, and in the negative control group, 100 μl of broth and bacterial soluti...

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Abstract

The invention belongs to the technical field of biology, and specifically discloses an antibacterial peptide PJF11 and application thereof to inhibition of peri-implantitis bacteria. The sequence of the antibacterial peptide PJF11 is shown as SEQ ID NO: 1; one end of a peptide chain of the antibacterial peptide is connected with acetate, and the other end of the peptide chain of the antibacterial peptide is connected with an amino group. The antibacterial peptide PJF11 is discovered to have obvious antibacterial activity to the peri-implantitis bacteria, is safe and nontoxic to human bodies, meanwhile has the characteristics of short peptide chain, easiness in synthesis, low cost and the like, and can be effectively applied to inhibiting the infection of oral cavity by the peri-implantitis bacteria, particularly the infection of fusobacterium nucleatum and staphylococcus aureus.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an antibacterial peptide PJF11 and its application in inhibiting peri-implantitis bacteria. Background technique [0002] Implant dentures have good stability and a high long-term success rate. However, with the increase in the number of patients receiving dental implant treatment, the incidence of peri-implantitis also increases rapidly. The key to preventing peri-implantitis is infection control. In addition to strict aseptic operation during the operation and teaching patients to maintain oral hygiene, it is even more important to prevent it with antibacterial drugs before inflammation occurs or when there is mild inflammation. treat. This is especially important in patients with a history of chronic periodontitis. Studies have shown that the probability of peri-implantitis in patients with periodontitis after implant treatment is significantly higher than that of pat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06A01N47/44A01P1/00
Inventor 金丰良许小霞高延富欧阳莉娜张玉清余静鞠雯燕杨涛刘长虹
Owner SOUTH CHINA AGRI UNIV
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