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Preparation method of hepatitis B virus surface protein adsorbent

A hepatitis B virus and surface protein technology, applied in chemical instruments and methods, alkali metal oxides/hydroxides, inorganic chemistry, etc., can solve problems such as easy detachment of ligands, and achieve improved safety and ligand binding Firmness, the effect of reducing the probability and rate of shedding

Inactive Publication Date: 2015-07-15
WUHAN RUIFA MEDICAL DEVICES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the defect that the ligands on the hepatitis B virus surface protein adsorbent prepared in the prior art are easy to fall off, and provide a kind of hepatitis B virus surface protein that is easy to operate and can greatly improve the binding firmness of the ligands. The preparation method of adsorbent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The preparation method of hepatitis B virus surface protein adsorbent comprises the following steps:

[0020] 1) preparing sodium ion taurocholic acid co-transporting polypeptide (NTCP) and carrier agarose microspheres with affinity to the surface protein of hepatitis B virus;

[0021] How to prepare NTCP and agarose microspheres is a published prior art, and will not be described in detail in this application.

[0022] 2) Activate the surface groups of agarose microspheres: Add 600ml of agarose microsphere solution and 400ml of 2mol / L NaOH aqueous solution into a 2L reactor, mix well, add 400ml of ethylene glycol bisglycidyl ether, and place at constant temperature In a shaker, react at 37°C for 1 hour. After the reaction was completed, the agarose microspheres were filtered, rinsed with a large amount of distilled water until pH = 7, and the activated gel-like agarose microspheres were stored at 4°C for future use.

[0023] 3) Connect NTCP to the upper agarose micro...

Embodiment 2

[0027] The preparation method of hepatitis B virus surface protein adsorbent comprises the following steps:

[0028] 1) preparing sodium ion taurocholic acid co-transporting polypeptide (NTCP) and carrier agarose microspheres with affinity to the surface protein of hepatitis B virus;

[0029] How to prepare NTCP and agarose microspheres is a published prior art, and will not be described in detail in this application.

[0030] 2) Activate the surface groups of agarose microspheres: Add 600ml of agarose microsphere solution and 400ml of 2mol / L NaOH aqueous solution into a 2L reactor, mix well, add 400ml of ethylene glycol bisglycidyl ether, and place at constant temperature In a shaker, react at 37°C for 1 hour. After the reaction was completed, the agarose microspheres were filtered, rinsed with a large amount of distilled water until pH = 7, and the activated gel-like agarose microspheres were stored at 4°C for future use.

[0031] 3) Connect NTCP to the upper agarose micro...

Embodiment 3

[0035] The preparation method of hepatitis B virus surface protein adsorbent comprises the following steps:

[0036] 1) preparing sodium ion taurocholic acid co-transporting polypeptide (NTCP) and carrier agarose microspheres with affinity to the surface protein of hepatitis B virus;

[0037] How to prepare NTCP and agarose microspheres is a published prior art, and will not be described in detail in this application.

[0038] 2) Activate the surface groups of agarose microspheres: Add 600ml of agarose microsphere solution and 400ml of 2mol / L NaOH aqueous solution into a 2L reactor, mix well, add 400ml of ethylene glycol bisglycidyl ether, and place at constant temperature In a shaker, react at 37°C for 1 hour. After the reaction was completed, the agarose microspheres were filtered, rinsed with a large amount of distilled water until pH = 7, and the activated gel-like agarose microspheres were stored at 4°C for future use.

[0039] 3) Connect NTCP to the upper agarose micro...

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Abstract

The invention discloses a preparation method of a hepatitis B virus surface protein adsorbent. The method uses amino acid treatment to connect adsorption microspheres of a ligand to make the ligand firmly combined, so the shedding probability of the hepatitis B virus surface protein adsorbent is reduced, and the application safety of the hepatitis B virus surface protein adsorbent is improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method of a hepatitis B virus surface protein adsorbent. Background technique [0002] Hepatitis B virus (HBV) belongs to the hepadnaviridae family, and its genome is about 3.2kb in length, which is partially double-stranded circular DNA. Due to the serious situation of hepatitis B virus in China, the country has formulated a vaccination system. However, studies have shown that many hepatitis B viruses have "immune escape" properties, that is, the ability to infect vaccinators, which leads to a gloomy prospect for the application of the vaccine. [0003] Studies have found that the toxin protein in the blood is the main pathogenic factor of hepatitis B, and the hepatitis B toxin protein mediates the infection of liver cells by hepatitis B virus. The prior art discloses an adsorption material for hepatitis B antigenic protein and a preparation method thereof. In the me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/22B01J20/28B01J20/30
Inventor 王业富韩振伟李卫
Owner WUHAN RUIFA MEDICAL DEVICES CO LTD
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