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Method for inoculating stock spawn of edible fungi

A technology for edible fungi and original species, applied in the directions of botanical equipment and methods, applications, fungi, etc., can solve the problems of small multiplying multiples, low efficiency and high cost, and achieve expansion of growth points, ensure physiological activity, and increase growth. point effect

Inactive Publication Date: 2015-07-15
青岛联合菌业科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the multiplication factor of traditional edible fungi is small, and large-scale planting requires more original species, which leads to high cost and low efficiency.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] An inoculation method for edible fungi stock, which includes the following steps,

[0030] 1. Making mother species:

[0031] (1) Making the mother seed culture material: 1L of the mother seed culture material is made up of 200g potatoes, 20g bran, 20g glucose, 20g agar, 1g potassium dihydrogen phosphate, 0.5g magnesium sulfate. In 18×20cm test tubes, each test tube is filled with 1 / 5 of the volume of the test tube, and sterilized at 121°C for 30 minutes;

[0032] (2) Inoculation: The test tube is placed on a slope while it is hot, and the inoculation starts after the temperature drops to room temperature. Before inoculation, the ultra-clean workbench is sterilized by ultraviolet light for 20 minutes, and the inoculation is performed in the ultra-clean workbench. Each test tube is inoculated with bacteria with an area of ​​3mm×3mm. Seed block

[0033] (3) Cultivation: The inoculated test tube is placed in a constant temperature shaker at 22°C for 15 days, and the test tube is ...

Embodiment 2

[0049] An inoculation method for edible fungi stock, which includes the following steps,

[0050] 1. Making mother species:

[0051] (1) Making the mother seed culture material: 1L of the mother seed culture material is made up of 200g potatoes, 20g bran, 20g glucose, 20g agar, 1g potassium dihydrogen phosphate, 0.5g magnesium sulfate. In 18×20cm test tubes, each test tube is filled with 1 / 5 of the volume of the test tube, and sterilized at 121°C for 30 minutes;

[0052] (2) Inoculation: The test tube is placed on a slope while it is hot, and the inoculation starts after the temperature drops to room temperature. Before inoculation, the ultra-clean workbench is sterilized by ultraviolet light for 20 minutes, and the inoculation is performed in the ultra-clean workbench. Each test tube is inoculated with bacteria with an area of ​​3mm×3mm. Seed block

[0053] (3) Cultivation: The inoculated test tube is placed in a constant temperature shaker at 25°C for 10 days, and the mycelium is o...

Embodiment 3

[0068] A method for inoculating edible fungi stock, which is characterized in that it includes the following steps:

[0069] 1. Making mother species:

[0070] (1) Making the mother seed culture material: 1L of the mother seed culture material is made up of 200g potatoes, 20g bran, 20g glucose, 20g agar, 1g potassium dihydrogen phosphate, 0.5g magnesium sulfate. In 18×20cm test tubes, each test tube is filled with 1 / 5 of the volume of the test tube, and sterilized at 121°C for 30 minutes;

[0071] (2) Inoculation: The test tube is placed on a slope while it is hot, and the inoculation starts after the temperature drops to room temperature. Before inoculation, the ultra-clean workbench is sterilized with ultraviolet light for 20 minutes, and the inoculation is performed in the ultra-clean workbench. Seed block

[0072] (3) Cultivation: The inoculated test tube is placed in a constant temperature shaker at 24°C for 12 days, and then the test tube can be overgrown and used to make the o...

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PUM

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Abstract

The invention relates to a method for inoculating stock spawn of edible fungi. The method comprises the steps: stock culture compost manufacturing; inoculating; culturing; compounding; loading; sterilizing; inoculating; culturing; sterile water manufacturing; conducting sterilization for 30 min at 121 DEG C by a pulverizer, after asepsis is guaranteed and the temperature is lowered to indoor temperature, taking out the compost from a stock spawn bacteria culturing bottle, wherein the stock spawn overgrows the compost, mixing the compost with the sterile water, grinding the mixture in the pulverizer, and mixing the sterile water with the compost overgrown by hyphae, wherein the proportion of the sterile water and the compost is one L sterile water is mixed with 50-100 g compost; filtrating the ground bacteria culture, taking a filtered bacterium solution, and diluting the bacterium solution by 80-100 times for production inoculation. By grinding the compost containing the stock spawn, the hyphae are segmented, the growing points of the edible fungi are greatly enlarged, the propagation expansion multiple is increased greatly, and the production cost is reduced. The sterile water is added in the grinding process, so that the grinding is more sufficient, the growing points of the edible fungi are further increased; the sterile water can serve as a great carrier of the stock spawn of the edible fungi, the physiological activity of the stock spawn of the edible fungi is guaranteed before culturing, and the fruiting rate of cultivated species is improved.

Description

Technical field [0001] The invention relates to the field of edible fungi production, in particular to an inoculation method of edible fungi stock. Background technique [0002] Edible fungus is rich in protein and amino acids, and its content is several to several tens of times that of ordinary vegetables and fruits. For example, fresh mushrooms contain 1.5-3.5% protein, which is 3 times that of Chinese cabbage, 6 times that of carrots, and 17 times that of apples. The protein contained in 1 kg of dried mushrooms is equivalent to 2 kg of lean meat, 3 kg of eggs or 12 kg of milk. Edible mushrooms are rich in lysine, containing 18 kinds of amino acids that make up protein, and 8 kinds of trace elements necessary for the human body. Lysine, which is low in cereal foods, is also abundant in edible fungi. The fat content of edible fungi is very low, accounting for about 0.2-3.6% of the dry weight, and 74-83% of them are unsaturated fatty acids that are beneficial to human health. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
CPCC12N1/14
Inventor 陈翠翠巩玉辉李坚之周绍辉隋海周
Owner 青岛联合菌业科技发展有限公司
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