Method for inoculating stock spawn of edible fungi
A technology for edible fungi and original species, applied in the directions of botanical equipment and methods, applications, fungi, etc., can solve the problems of small multiplying multiples, low efficiency and high cost, and achieve expansion of growth points, ensure physiological activity, and increase growth. point effect
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Embodiment 1
[0029] An inoculation method for edible fungi stock, which includes the following steps,
[0030] 1. Making mother species:
[0031] (1) Making the mother seed culture material: 1L of the mother seed culture material is made up of 200g potatoes, 20g bran, 20g glucose, 20g agar, 1g potassium dihydrogen phosphate, 0.5g magnesium sulfate. In 18×20cm test tubes, each test tube is filled with 1 / 5 of the volume of the test tube, and sterilized at 121°C for 30 minutes;
[0032] (2) Inoculation: The test tube is placed on a slope while it is hot, and the inoculation starts after the temperature drops to room temperature. Before inoculation, the ultra-clean workbench is sterilized by ultraviolet light for 20 minutes, and the inoculation is performed in the ultra-clean workbench. Each test tube is inoculated with bacteria with an area of 3mm×3mm. Seed block
[0033] (3) Cultivation: The inoculated test tube is placed in a constant temperature shaker at 22°C for 15 days, and the test tube is ...
Embodiment 2
[0049] An inoculation method for edible fungi stock, which includes the following steps,
[0050] 1. Making mother species:
[0051] (1) Making the mother seed culture material: 1L of the mother seed culture material is made up of 200g potatoes, 20g bran, 20g glucose, 20g agar, 1g potassium dihydrogen phosphate, 0.5g magnesium sulfate. In 18×20cm test tubes, each test tube is filled with 1 / 5 of the volume of the test tube, and sterilized at 121°C for 30 minutes;
[0052] (2) Inoculation: The test tube is placed on a slope while it is hot, and the inoculation starts after the temperature drops to room temperature. Before inoculation, the ultra-clean workbench is sterilized by ultraviolet light for 20 minutes, and the inoculation is performed in the ultra-clean workbench. Each test tube is inoculated with bacteria with an area of 3mm×3mm. Seed block
[0053] (3) Cultivation: The inoculated test tube is placed in a constant temperature shaker at 25°C for 10 days, and the mycelium is o...
Embodiment 3
[0068] A method for inoculating edible fungi stock, which is characterized in that it includes the following steps:
[0069] 1. Making mother species:
[0070] (1) Making the mother seed culture material: 1L of the mother seed culture material is made up of 200g potatoes, 20g bran, 20g glucose, 20g agar, 1g potassium dihydrogen phosphate, 0.5g magnesium sulfate. In 18×20cm test tubes, each test tube is filled with 1 / 5 of the volume of the test tube, and sterilized at 121°C for 30 minutes;
[0071] (2) Inoculation: The test tube is placed on a slope while it is hot, and the inoculation starts after the temperature drops to room temperature. Before inoculation, the ultra-clean workbench is sterilized with ultraviolet light for 20 minutes, and the inoculation is performed in the ultra-clean workbench. Seed block
[0072] (3) Cultivation: The inoculated test tube is placed in a constant temperature shaker at 24°C for 12 days, and then the test tube can be overgrown and used to make the o...
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