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Method for rapidly preparing galactomannan oligosaccharide enzymatic hydrolysate by utilizing high-concentration guar gum solution

The technology of mannose enzymolysis solution and guar gum is applied in the biological field, which can solve the problems of long time, low enzymolysis efficiency, low oligosaccharide, etc., to save production time and cost, fast enzymolysis speed and improve efficiency. Effect

Inactive Publication Date: 2015-07-01
QINGZHOU RONMER BIOLOGY TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One is that the existing method mainly adopts β-mannanase to the enzymatic hydrolysis of guar gum, but the efficiency of adopting a single enzyme to hydrolyze guar gum is not high
Although some existing technologies use the method of degrading plant polysaccharides with compound enzymes, since each enzyme degrades separately, it takes a long time and the enzymatic hydrolysis efficiency is low
The 2nd, in the guar gum enzymatic hydrolysis process, because the guar gum solution viscosity is too high, the concentration when the enzymolysis guar gum generally adopts at present is 0.3-2%, the concentration of the oligosaccharide that obtains is lower, in further When preparing galactomannose powder, dehydration and drying treatment is required, which not only increases the cost of subsequent dehydration and drying of the product, galactomannose, but also seriously affects the efficiency of industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take 12g of guar gum powder, dilute to 100 mL with 0.2mol / mL citric acid-sodium citrate buffer solution, adjust the pH value to 6.0; then heat and swell at 92°C for 50min; cool to 25°C , add 840U of β-mannanase, 12000U of xylanase and 24000U of endoglucanase at the same time, and mix thoroughly;

[0025] After adding the three enzymes, the temperature gradient enzymatic hydrolysis method is adopted, specifically:

[0026] (1) Raise the temperature from the cooled temperature to 35°C, and perform enzymatic hydrolysis for 1.5 hours;

[0027] (2) Raise the temperature to 48°C and perform enzymatic hydrolysis for 3 hours; during the heating process, increase the temperature by 1°C every 3 minutes, and keep it for 10 minutes for every 1°C increase;

[0028] (3) Raise the temperature to 55°C and perform enzymatic hydrolysis for 2 hours.

[0029] After the enzymatic hydrolysis, the oligogalactomannose enzymatic hydrolysis solution is obtained. The mass percentages of galact...

Embodiment 2

[0033] Take 13g of guar gum powder, dilute to 100 mL with 0.2mol / mL citric acid-sodium citrate buffer solution, adjust the pH value to 6.3; then heat and swell at 93.5°C for 55min; after cooling to 28°C At the same time, add 1040U of β-mannanase, 19500U of xylanase, and 32500U of endoglucanase, and mix well.

[0034] After adding the three enzymes, the temperature gradient enzymatic hydrolysis method is adopted, specifically:

[0035] (1) Raise the temperature from the cooled temperature to 37°C, and perform enzymatic hydrolysis for 2 hours;

[0036] (2) Raise the temperature to 50°C and perform enzymatic hydrolysis for 3.5 hours; increase the temperature by 1.5°C every 4 minutes during the heating process, and keep for 10 minutes for every 1.5°C increase;

[0037] (3) Raise the temperature to 55°C and perform enzymatic hydrolysis for 2.5 hours.

[0038] After the enzymatic hydrolysis, the oligogalactomannose enzymatic hydrolysis solution is obtained. The mass percentages o...

Embodiment 3

[0042] Take 15g of guar gum powder, dilute to 100 mL with 0.2mol / mL citric acid-sodium citrate buffer solution, adjust the pH value to 6.6; then heat and swell at 95°C for 60min; after cooling to 30°C , while adding β-mannanase 1350U, xylanase 30000U, endoglucanase 45000U, mixed well.

[0043] After adding the three enzymes, the temperature gradient enzymatic hydrolysis method is adopted, specifically:

[0044] (1) Raise the temperature from the cooled temperature to 40°C, and perform enzymatic hydrolysis for 2.5 hours;

[0045] (2) Raise the temperature to 53°C and perform enzymatic hydrolysis for 4 hours; during the heating process, increase the temperature by 2°C every 5 minutes, and keep it for 10 minutes for every 2°C increase;

[0046] (3) Raise the temperature to 55°C and perform enzymatic hydrolysis for 3 hours.

[0047] After the enzymatic hydrolysis, the oligogalactomannose enzymatic hydrolysis solution is obtained. The mass percentages of galactomannose oligosacc...

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Abstract

The invention discloses a method for rapidly preparing galactomannan oligosaccharide enzymatic hydrolysate by utilizing a high-concentration guar gum solution. The method is characterized by firstly preparing a guar gum solution with a citric acid-sodium citrate buffer solution, then controlling the temperature and time to carry out heating swelling, after cooling, adding beta-mannanase, xylanase and endoglucanase at the same time, fully mixing the materials uniformly and controlling the enzymolysis temperature and time to carry out degradation. The method has the effects of achieving rapid enzymolysis of guar gum at high concentration, obtaining the galactomannan oligosaccharide enzymatic hydrolysate and saving the drying and purifying costs during subsequent production of galactomannan oligosaccharide.

Description

technical field [0001] The invention relates to a method for quickly preparing a high-concentration guar gum solution for enzymatic hydrolysis of oligogalactomannose, and belongs to the field of biotechnology. Background technique [0002] Guar gum is a natural thickener and quality improver processed from the endosperm of guar beans, an annual herbaceous leguminous crop. The main component is galactomannan. Guar gum is a white or yellowish free-flowing powder, soluble in cold or hot water, and forms a jelly-like substance when it meets water, which can thicken rapidly. The backbone of the galactomannan structure is mannan linked together by β-(1-4) glycosidic bonds, and the branched chains are galactans linked by α-(1-6) glycosidic bonds. Galactomannose is an incomplete degradation product of galactomannan. It is a general term for oligosaccharides polymerized by 2-10 galactose and mannose molecules through glycosidic bonds. The molar ratio of galactose and mannose About ...

Claims

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Application Information

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IPC IPC(8): C12P19/14
Inventor 刘云国张亮荣宝刚
Owner QINGZHOU RONMER BIOLOGY TECH CO LTD
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