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Method for preparing selenium nano-particles by taking secretion of microbial cells as matrix

A technology of microbial cells and secretions, which is applied in the fields of material synthesis, nanoscience, and microbiology, and achieves the effects of low cost, simple preparation process, and simple operation

Inactive Publication Date: 2015-07-01
HENAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there has been no report on the method of preparing selenium nanoparticles in microorganisms as a substrate

Method used

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  • Method for preparing selenium nano-particles by taking secretion of microbial cells as matrix
  • Method for preparing selenium nano-particles by taking secretion of microbial cells as matrix

Examples

Experimental program
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Effect test

Embodiment 1

[0016] Yeast cell culture: Inoculate a single yeast colony into 30mL of water, add a glucose solution with a mass concentration of 0.2%, put it in a constant temperature and constant speed shaker, and pour 1mM selenium powder quickly after shaking for 24 hours, and place it at a temperature of 20 ℃, the shaking speed is 100r / min, shaking for 3h, centrifugal washing and drying to prepare nano-selenium particles with a particle size of 3-8nm. figure 1 It is the XRD collection of illustrative plates of the selenium nanoparticle that the present embodiment makes, and the XRD collection of collections of collection of known product and selenium is consistent as shown in the figure, figure 2 It is a TEM image of the selenium nanoparticles prepared in this embodiment, and it can be seen from the figure that the particle size of the selenium nanoparticles prepared is 3-8nm.

Embodiment 2

[0018] Cultivate Lactobacillus cells: inoculate a single colony of Lactobacillus into 40mL of water, add 0.2% sucrose solution, place in a constant temperature and constant speed shaker, shake for 24 hours, and quickly pour 1mM Na 2 SeO 3 The prepared solution was shaken at a temperature of 25° C. at a shaking speed of 200 r / min for 5 hours, washed by centrifugation, and dried to obtain nano-selenium particles with a particle size of 3-8 nm.

Embodiment 3

[0020] Yeast cell culture: Inoculate a single colony of yeast into 40mL of water, add a lentinan solution with a mass concentration of 0.4%, put it in a constant temperature and constant speed shaker, shake it for 24 hours, and quickly pour the solution prepared by 2mM selenic acid , at a temperature of 25° C., a shaking speed of 200 r / min, shaking for 5 hours, centrifuging, washing, and drying to prepare nano-selenium particles with a particle size of 3-8 nm.

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Abstract

The invention discloses a method for preparing selenium nano-particles by taking secretion of microbial cells as a matrix, belonging to the technical field of synthesis of nano-particles. The method for preparing selenium nano-particles by taking secretion of microbial cells as a matrix is technically characterized by comprising the following steps: inoculating 30-100mL of water with microbial single colonies and adding a sugary solution with mass concentration of 0.2-1.6%; oscillating for 24h, then adding 1-5mmol of selenium powder, selenic acid or sodium selenite, putting the mixed solution in a constant-temperature constant-speed shaker and oscillating for 3-24h at 20-60 DEG C at an oscillating speed of 100-600r / min; and centrifuging, collecting precipitate, washing and drying to obtain selenium nano-particles with particle size of 3-8nm. The method disclosed by the invention has the advantages of environment friendliness, good biocompatibility, simple preparation process and the like.

Description

technical field [0001] The invention belongs to the technical fields of nanoscience, microbiology and material synthesis, and in particular relates to a method for preparing selenium nanoparticles by using microbial cell secretion as a substrate. Background technique [0002] When the particle size enters the nanometer level, it has quantum size effect, surface effect, volume effect, macroscopic quantum tunneling effect, and dielectric confinement effect, so it exhibits a series of novel physical and chemical properties, and involves volume Some basic physical and chemical problems that are ignored or not at all in phase materials. Therefore, as a new material, nanomaterials have shown broad application prospects in the fields of information storage, catalysis, light absorption, luminescence, and medicine. Selenium has a low melting point and high conductivity. It is an important semiconductor material. It is used in solar cells, rectifiers, toner cartridges and other field...

Claims

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Application Information

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IPC IPC(8): C12P3/00C12R1/77C12R1/89C12R1/01
Inventor 马晓明杨媛媛杨林王魁朱郁葱
Owner HENAN NORMAL UNIV
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