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Establishment method for pig unilateral ureter obstruction recanalization model

A technology of ureteral obstruction and establishment method, applied in the field of establishment of animal models, to achieve the effects of easy acquisition, low price, and avoiding repeated stretching

Inactive Publication Date: 2015-06-24
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the obstructive factors of obstructive nephropathy are relieved, the kidney will recover to varying degrees in terms of shape and function. However, there are few studies on the lesions or repair process of the kidney after UUO obstruction is relieved and recanalized.

Method used

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  • Establishment method for pig unilateral ureter obstruction recanalization model
  • Establishment method for pig unilateral ureter obstruction recanalization model
  • Establishment method for pig unilateral ureter obstruction recanalization model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Include the following steps:

[0029](1) Select experimental animals, the selected experimental pigs are Guangxi Bamaxiang pigs, 3 months old, 8-10 kg in body weight, raised in accordance with standard animal feeding conditions, 18-25 ° C, 12 hours of light / dark cycle, every Pigs are fed pig feed twice a day, 250g each time;

[0030] (2) Modeling operation, the experimental animals were fasted for 12 hours before the operation, and combined anesthesia with diazepam, ketamine and Sumianxin II. The specific operation process of anesthesia: first intramuscularly inject 20 mg / kg of ketamine, and shake the animal after 50 seconds After falling to the ground and muscle relaxation, intramuscular injection of diazepam 1mg / kg and Sumianxin 0.15ml / kg were given intramuscularly, and the anesthesia took effect within 110 seconds. The pig was placed on a constant temperature operating table at 37°C, and all surgical procedures followed aseptic procedures. Routine skin preparation, ...

Embodiment 2

[0034] Include the following steps:

[0035] (1) Select experimental animals, the selected experimental pigs are Guangxi Bamaxiang pigs, 3 months old, 8-10 kg in body weight, raised in accordance with standard animal feeding conditions, 18-25 ° C, 12 hours of light / dark cycle, every Pigs are fed pig feed twice a day, 250g each time;

[0036] (2) Modeling operation, the experimental animals were fasted for 12 hours before the operation, and combined anesthesia with diazepam, ketamine and Sumianxin II. The specific operation process of anesthesia: first give ketamine 20mg / kg intramuscular injection, and shake the animal after 60 seconds After falling to the ground and muscle relaxation, intramuscular injection of diazepam 1mg / kg and Sumianxin 0.15ml / kg were given intramuscularly, and the anesthesia took effect within 120 seconds. The pig was placed on a constant temperature operating table at 37°C, and all surgical procedures followed aseptic procedures. Routine skin preparatio...

Embodiment 3

[0040] Include the following steps:

[0041] (1) Select experimental animals, the selected experimental pigs are Guangxi Bamaxiang pigs, 3 months old, 8-10 kg in body weight, raised in accordance with standard animal feeding conditions, 18-25 ° C, 12 hours of light / dark cycle, every Pigs are fed pig feed twice a day, 250g each time;

[0042] (2) Modeling operation, the experimental animals were fasted for 12 hours before the operation, and combined anesthesia with diazepam, ketamine and Sumianxin II. The specific operation process of anesthesia: first intramuscularly inject 20 mg / kg of ketamine, and shake the animal after 70 seconds After falling to the ground and muscle relaxation, intramuscular injection of diazepam 1mg / kg and Sumianxin 0.15ml / kg were given intramuscularly, and the anesthesia took effect within 130 seconds. The pig was placed on a constant temperature operating table at 37°C, and all surgical procedures followed aseptic procedures. Routine skin preparation,...

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Abstract

The invention relates to an establishment method for a pig unilateral ureter obstruction recanalization model, and belongs to an establishment method for animal models. A Bama minature pig is selected, combined anesthesia is adopted, a polyethylene piece is curled into a polyethylene pipe to be wound around a kidney ureter, and the two ends of the ureter are ligatured through the number 10 surgery sutures so that the right side ureter can be completely obstructed. The method has the advantage that the pig unilateral ureter obstruction recanalization model is successfully established, a good experimental platform is provided for the research on renal interstitial fibrosis, and meanwhile a good tool is provided for medicine experiments through the model; through the analysis on serum markers and pathology of all time points of obstruction and recanalization, the changes, of different degrees, happening to a kidney on the aspects of general pathology and physiological functions when obstruction recanalization is achieved at different time points can be easily found.

Description

technical field [0001] The invention belongs to a method for establishing an animal model, in particular to a method for establishing a pig unilateral ureteral obstruction recanalization model. Background technique [0002] Renal interstitial fibrosis is the last common pathway for all renal diseases to develop to the end stage. The mechanism of renal interstitial fibrosis is a complex pathophysiological process. Many cell mediators and growth factors are involved in this process, but there is still a lack of comprehensive understanding of its mechanism. The resulting end-stage renal disease requires Hemodialysis treatment is a heavy burden on patients, families and society. Therefore, it is of great significance to elucidate the pathogenesis of renal interstitial fibrosis and find its intervention targets. [0003] At present, there are many studies on the models of renal interstitial fibrosis, including 5 / 6 nephrectomy model, aristolochic acid model, uranyl nitrate model...

Claims

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Application Information

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IPC IPC(8): A61D1/00
CPCA61D1/00A61D3/00
Inventor 孙晶刘盈盈纪磊刘树军李兵罗曼宇王杨威董文鹏苗里宁
Owner JILIN UNIV
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