Strain for promoting germination and seedling formation of Dendrobium devonianum seed and application thereof

Abstract

The invention discloses a strain for promoting germination and seedling formation of a Dendrobium devonianum seed and application thereof. The classification designation of the strain is Tulasnella sp.CY collected to China General Microbiological Culture Collection Center, wherein the collection number is CGMCC NO.9551. The strain can obviously promote germination and seedling formation of the Dendrobium devonianum seed, and has higher specificity. The strain has very import practical meanings for acquiring the symbiotic seed and seedling of Dendrobium devonianum through seed, can be used for nursery seedling raising or outdoor trunk seed direct sowing, has the advantages of low cost, high outdoor survival rate, no tissue degradation and the like as compared with tissue culture, has the application value of acquiring the symbiotic seed and seedling instead of tissue culture, and provides reliable technical guarantees for solving the bottleneck problem of seed and seedling sources in the Dendrobium devonianum culture industry.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and in particular relates to a fungus strain of the genus Colloidal which can effectively promote the symbiotic germination of dendrobium dendrobium seeds to form protocorms, and grow and develop a large number of seedlings. At the same time, the invention also relates to the specific application of the bacterial strain in the breeding of Dendrobium dendrobium. Background technique [0002] As a traditional Chinese medicinal material, Dendrobium devonianum has extremely high medicinal value, and its cultivation scale has developed into the second pillar industry after Dendrobium candidum in some prefectures of Yunnan. There are roughly four sources of seedlings used in the industry: the first is wild plants, which were mainly seen in some small individual enterprises in the 1990s. With the scarcity of wild resources, this practice cannot continue. For the protection of plants, it is ill...

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Problems solved by technology

There are roughly four sources of seedlings used in the industry: the first is wild plants, which were mainly seen in some small individual enterprises in the 1990s. With the scarcity of wild resources, this practice cannot continue. For the protection of plants, it is illegal to collect wild plants for trading; the second method is to propagate seedlings by cuttings, which requires a lot of manpower, and the reproductive coefficient of seedlings is extremely low, which is not suitable for large-scale industrial development; the third method Seedlings are obtained by aseptic germination of seeds. The technology is relatively mature. Although the germination rate is high, the survival rate of seedlings is low during the process of going from a sterile environment to the outdoors, and because they cannot establish a symbiotic relationship with fungi in nature, there are slow growth in the later stage, etc. Fatal defect; currently the fourth method is mainly used, namely tissue culture method. Although a large number of seedlings can be obtained, certain technical exploration and a large amount of manpower are required in the process of seedling hardening and transplanting. During the growth of seedlings, it is necessary to build greenhouses, Basic investment such as modern water spraying facilities, what's more, a large amount of pesticides and chemical fertilizers need to be sprayed, resulting in a decrease in the quality of seedlings

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