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A kind of oligomeric guluronic acid phosphate and its preparation method and application

A technology of guluronic acid phosphate and polyguluronic acid, which is applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problem of high industrialization cost, achieve low production cost, Significant immune enhancement, the effect of enhancing humoral immunity and cellular immunity

Active Publication Date: 2018-09-18
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the high cost of industrialization of polysaccharide immunomodulators and the current research status of polysaccharide phosphate and oligomeric guluronic acid phosphate, the invention provides a kind of oligomeric guluronic acid phosphate and its Preparation method and application

Method used

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  • A kind of oligomeric guluronic acid phosphate and its preparation method and application
  • A kind of oligomeric guluronic acid phosphate and its preparation method and application
  • A kind of oligomeric guluronic acid phosphate and its preparation method and application

Examples

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Embodiment 1

[0025] Example 1: Preparation of oligomeric guluronic acid phosphate

[0026] The raw material used in the present invention is oligomeric guluronic acid (purity>80%, weight-average molecular weight<20kDa) which is obtained through degradation and classification of alginate, and existing oligomeric guluron in the prior art can also be used aldehyde acid. Weigh 10 g of oligomeric guluronic acid into a reaction flask, add 180 g of urea, add 800 mL of N,N-dimethylformamide, and then add 70 mL of phosphoric acid (mass volume ratio is 85%), and stir evenly. The suspension formed by mixing the above components was refluxed at 130° C. for 4 hours. After the reaction is over, collect the reaction precipitate, wash it repeatedly with 95% ethanol and dissolve it in water, adjust the pH of the solution to 8-9 with NaOH, then use semi-permeable membrane dialysis to remove residual urea and phosphate during the reaction, spray dry, The oligomeric guluronic acid phosphate is obtained.

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Embodiment 2

[0036] Example 2: Immunopotentiation of oligomeric guluronic acid phosphate

[0037] According to the internationally recognized method, the immune enhancing effect of oligomeric guluronic acid phosphate was evaluated at the animal level.

[0038] 1) Effects on body weight and immune organ / body weight ratio of immunocompromised mice

[0039] Take 72 SPF-level Kunming mice (18-22g) and feed them adaptively for 3 days, and then randomly divide them into 6 groups, 12 mice in each group, which are blank group, model group, low polyguluronic acid phosphate , medium and high dose groups and lentinan group. The administration was administered by intragastric administration for 10 consecutive days; except for the blank group, the mice in the other groups were intraperitoneally injected with cyclophosphamide 100 mg / kg·d on the sixth day of administration to establish a model. 24 hours after the last administration, the mice were weighed, sacrificed by cervical dislocation, the spleen a...

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Abstract

The invention discloses an oligomeric guluronic acid phosphate as well as a preparation method and an application thereof. The molecular framework of oligomeric guluronic acid phosphate is a linear polymer of L-guluronic acid connected through alpha-(1,4)-glucosidic bonds, in the formula, R1 is pharmaceutical salt such as H, Li, Na, K, Ca or Mg, R2 is H or PO3<2->, the salting mode of -PO32- is accordant with that when R1 is taken as a substituent group, and n is less than or equal to 20. By adopting the oligomeric guluronic acid phosphate, the situation of low immunity of mice caused by cyclophosphamide can be alleviated, the major performance includes that the immune viscera / weight ratio can be increased, the humoral immunity and the cell immunity can be improved, the phagocytic ability of monocyte macrophages is improved, and the numbers of leukocytes and lymphocytes in peripheral hemogram can be remarkably increased. The pharmacology experiment result shows that the oligomeric guluronic acid phosphate disclosed by the invention can be used for preparing medicines, food or functional products with immunological enhancement.

Description

technical field [0001] The invention belongs to the field of marine drugs and immune enhancers, and in particular relates to an oligomeric guluronic acid phosphate and its preparation method and application. Background technique [0002] Polysaccharides are a class of natural biological response modifiers (Biological response modifiers, BRMs), which can enhance the body's specific and non-specific immune functions. For example, lentinan and astragalus polysaccharides are used as immune Enhancers are used in pharmaceuticals or functional products. However, most of the separation and purification processes of these polysaccharides are relatively cumbersome, and the industrialization cost is relatively high. [0003] In recent years, polysaccharide phosphates, as a class of polysaccharide derivatives with special structures, have been reported to have biological activities such as immune regulation, anti-tumor, and anti-oxidation. In 1998, the Kitazawa group of Tohoku Univers...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C07H11/04C07H1/00A61P37/04A23L33/125A23L33/10
Inventor 李春霞管华诗李全才辛萌李海花于广利
Owner OCEAN UNIV OF CHINA
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