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Efficient microflow electrochromatography separation method of plant growth regulator containing carboxyl

A plant growth regulator and micro-current chromatography technology, applied in the field of analytical chemistry, can solve problems such as retention and weak hormone retention, and achieve the effect of increasing polarity and ionic charge intensity

Active Publication Date: 2015-05-20
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the separation of plant growth regulators by capillary electrochromatography is still mainly based on the reversed-phase chromatographic separation and electric field-driven migration of organic polymers on the surface of traditional capillary packing columns. Carboxylic acid phytohormones have weak retention and need to be improved

Method used

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  • Efficient microflow electrochromatography separation method of plant growth regulator containing carboxyl
  • Efficient microflow electrochromatography separation method of plant growth regulator containing carboxyl
  • Efficient microflow electrochromatography separation method of plant growth regulator containing carboxyl

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Experimental program
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Effect test

Embodiment 1

[0023] Based on the capillary electrochromatography system, the octadecyl bonded silica gel capillary packed column is used for separation. The specific method is: apply a negatively charged high-voltage electric field at the end of the capillary column and apply a liquid phase pump auxiliary pressure on the injection port, and the injection volume is 20 μL, using acetonitrile: sodium chloride solution = 70:30 (v / v) as the mobile phase, the concentration of sodium chloride is 3.0 mmol / L, the separation voltage is +8.0kV, the auxiliary pressure of the pump is 1000 psi, and the flow rate is 0.05 mL / min. The elution peaks of plant growth regulators are: 2,4a,7-trihydroxy-1-methyl-8-methylene gibberellin-3-ene-1,10-dicarboxylic acid-1,4a-lactone (peak 1); 2-cis,4-trans-5-(1-hydroxy-4-oxo-2,6,6-trimethyl-2-cyclohexen-1-yl)-3- Methyl-2,4-napadienoic acid (peak 2); 2,4-dichlorophenoxyacetic acid (peak 3).

Embodiment 2

[0025] Based on the capillary electrochromatography system, the octadecyl bonded silica gel capillary packed column is used for separation. The specific method is: apply a negatively charged high-voltage electric field at the end of the capillary column and apply a liquid phase pump auxiliary pressure on the injection port, and the injection volume is 20 μL, using acetonitrile: buffer solution = 70:30 (v / v) as the mobile phase, the electrolytes in the buffer solution are ammonium formate, sodium bisulfate, sodium bisulfite, the concentration is 3.0 mmol / L, the separation voltage +8.0kV, pump auxiliary pressure 1000 psi, flow rate 0.05 mL / min, capillary electrochromatographic separation of plant growth regulators, when sodium bisulfite is used as the electrolyte of the mobile phase, the elution peaks are: 2, 4a, 7 -Trihydroxy-1-methyl-8-methylenegibber-3-ene-1,10-dicarboxylic acid-1,4a-lactone (peak 1); 2-cis,4-trans- 5-(1-Hydroxy-4-oxo-2,6,6-trimethyl-2-cyclohexen-1-yl)-3-meth...

Embodiment 3

[0027] Based on the capillary electrochromatography system, the octadecyl bonded silica gel capillary packed column is used for separation. The specific method is: apply a negatively charged high-voltage electric field at the end of the capillary column and apply a liquid phase pump auxiliary pressure on the injection port, and the injection volume is 20 μL, using acetonitrile: buffer solution = 70:30 (v / v) as the mobile phase, the electrolytes in the buffer solution are sodium sulfate, sodium bicarbonate, sodium chloride, the concentration is 3.0 mmol / L, the separation voltage + 8.0kV, pump auxiliary pressure 1000 psi, flow rate 0.05 mL / min, capillary electrochromatographic separation of plant growth regulators, when sodium chloride is used as the electrolyte of the mobile phase, the elution peaks are: 2, 4a, 7-three Hydroxy-1-methyl-8-methylenegibber-3-ene-1,10-dicarboxylate-1,4a-lactone (peak 1); 2-cis,4-trans-5- (1-Hydroxy-4-oxo-2,6,6-trimethyl-2-cyclohexen-1-yl)-3-methyl-...

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Abstract

The invention discloses an efficient microflow electrochromatography separation method of a plant growth regulator containing carboxyl. Based on an inverse-phase capillary column, a compound containing a nucleophilic group is used as moving phase electrolyte to dynamically modify a reversed-phase column filler so as to form a nucleophilic base separation column; a high-voltage electric field and a micro-flow liquid phase analysis mode are applied to the columns to efficiently separate the plant growth regulator containing carboxyl. The method is characterized in that comprehensive functions of electro-osmotic flow, electrophoresis and polar chromatography are easy to achieve, and capillary electrophoresis separation of the polar carboxyl plant growth regulator and absorption chromatography separation of the nucleophilic group are realized.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a high-efficiency micro-current chromatographic separation method for carboxyl-containing plant growth regulators. Background technique [0002] Plant growth regulators are synthetic chemical substances used to regulate plant growth and development. They are mainly divided into plant growth promoters, plant growth retardants, and plant growth inhibitors. Among them, 2,4a,7-trihydroxy-1-methyl -8-methylene gibberellin-3-ene-1,10-dicarboxylic acid-1,4a-lactone belongs to gibberellin growth hormone, 2-cis,4-trans-5-(1- Hydroxy-4-oxo-2,6,6-trimethyl-2-cyclohexen-1-yl)-3-methyl-2,4-pentadienoic acid is a sesquiterpene structure The plant hormone, 2,4-dichlorophenoxyacetic acid belongs to the auxin analogues and is widely used in production. [0003] Regarding the detection of carboxylic acid plant growth regulators, mainly high performance liquid chromatography, liqui...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 林旭聪王绪培谢增鸿
Owner FUZHOU UNIV
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