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Crateva unilocalaris Buch. polysaccharide extraction and purification method and application of crateva unilocalaris Buch. polysaccharide

The technology of a polysaccharide extraction and purification method, which is applied in the field of extraction and purification of polysaccharide from cabbage, can solve problems such as research on the extraction process of cabbage polysaccharide.

Inactive Publication Date: 2015-05-20
中华人民共和国北仑出入境检验检疫局
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on tree head vegetable is mainly based on chemical components and analysis methods, and there is no research on the extraction process of tree head vegetable polysaccharides

Method used

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  • Crateva unilocalaris Buch. polysaccharide extraction and purification method and application of crateva unilocalaris Buch. polysaccharide
  • Crateva unilocalaris Buch. polysaccharide extraction and purification method and application of crateva unilocalaris Buch. polysaccharide
  • Crateva unilocalaris Buch. polysaccharide extraction and purification method and application of crateva unilocalaris Buch. polysaccharide

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Experimental program
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Effect test

specific Embodiment 1

[0028] 1. Materials, reagents and equipment

[0029] The tender stems and leaves of tree head vegetables are collected from Yunlongjian Scenic Area in Lianyungang City; ethanol, anhydrous ether, acetone, sulfuric acid, phenol, glucose, ascorbic acid (VC), tert-butylhydroquinone (TBHQ), pyrogallol, Salicylic acid, Tris (Tris), potassium ferricyanide, trichloroacetic acid, ferric chloride, and ferrous sulfate are all analytically pure from Shanghai Sinopharm Group. UV-2550 ultraviolet spectrophotometer (Shimadzu Corporation); RE-5285A rotary evaporator (Shanghai Yarong Biochemical Instrument Factory); DHG-9240A electric constant temperature blast drying oven (Shanghai Yiheng Technology Co., Ltd.); TDL-4 desktop centrifuge (Shanghai Anting Scientific Instrument Factory).

[0030] 2. The method for extracting and purifying polysaccharides from tree head vegetable, the specific steps are as follows:

[0031] Take the tree head vegetable and carry out microwave dehydration at room...

specific Embodiment 2

[0032] Determination of optimal extraction conditions

[0033] 1. Single factor test

[0034]Select extraction temperature (60-100°C), extraction time (2-4h), liquid-to-solid ratio (10-30:1mL / g), particle size (20-80 mesh) to conduct a single factor test.

[0035] 1.1 The effect of extraction temperature on the extraction rate of crude polysaccharides

[0036] Under the conditions of extraction time 3h, liquid-solid ratio 25:1, and extraction once, the influence of different extraction temperatures on the extraction rate of polysaccharides was investigated, and the results were as follows figure 1 shown. With the increase of extraction temperature, the extraction rate of crude polysaccharides from Arboria arborifolia increased rapidly, and when the temperature reached 80°C, the extraction rate of polysaccharides increased slowly, so the extraction temperature was preferably 90°C.

[0037] 1.2 The effect of liquid-solid ratio on the extraction rate of crude polysaccharides ...

specific Embodiment 3

[0055] Determination of Antioxidant Activity of Barkweed Polysaccharides

[0056] The extracted crude polysaccharides of barkweed were dissolved in water and purified repeatedly by Sevag method to remove impurities such as protein and nucleic acid until there were no characteristic absorption peaks at 260 nm and 280 nm by ultraviolet detection. Then the precipitate was washed three times with acetone and ethyl acetate, and dried at low temperature to obtain the polysaccharide product of barkweed with a purity of 99.2%, which was used for the determination of antioxidant activity. With vitamin C (V C ) and TBHQ as a control, the reducing power, hydroxyl radical scavenging rate and superoxide anion free radical scavenging rate were measured on the purified polysaccharides of barkweed.

[0057] 1. The reducing ability of tree head vegetable polysaccharide

[0058] By test results Figure 5 It can be seen that within the range of the experimental mass concentration, the reducin...

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Abstract

The invention discloses a crateva unilocalaris Buch. polysaccharide extraction and purification method and application of crateva unilocalaris Buch. polysaccharide. The method is characterized by comprising the following steps: performing microwave dehydration of crateva unilocalaris Buch., wherein the microwave power is 650W, and the dehydration time is 10min; milling the dehydrated crateva unilocalaris Buch. into dry crateva unilocalaris Buch. powder with the particle size of 40 meshes through a knife mill; placing the dry crateva unilocalaris Buch. powder in a reflux device, adding an ethanol solution with the volume concentration of 95%; performing reflux extraction for 3.6h at a constant temperature 97DEG C to obtain crude crateva unilocalaris Buch. polysaccharide; dissolving the crude crateva unilocalaris Buch. polysaccharide in water; purifying the crude crateva unilocalaris Buch. polysaccharide repeatedly by a Sevag method until the crude crateva unilocalaris Buch. polysaccharide has no characteristic absorption peak at 260nm and 280nm in an ultraviolet test; then performing washing and precipitation 3 times with acetone and ethyl acetate in sequence; and finally performing drying at 50DEG C to obtain crateva unilocalaris Buch. polysaccharide with the purity of 99.2%. The crateva unilocalaris Buch. polysaccharide can be applied to preparation of an antioxidant and has the advantages of high extraction efficiency, high product purity and high antioxidant performance.

Description

technical field [0001] The invention relates to a method for extracting polysaccharides, in particular to a method for extracting and purifying barkweed polysaccharides and its application. Background technique [0002] Tree head vegetable (crateva unilocalaris Buch.) is also known as spiny bract vegetable. Most of it grows wild in mountainous areas such as Yunnan, Guangdong and Guangxi in my country. It is known as forest vegetable. The tree head vegetable looks like Chinese toon, and it is mostly picked and eaten in spring. It is an umbrella-shaped tender tip of a thorny vine tree, plump and fragrant. Since tree head vegetables mostly grow in humid mountainous areas at an altitude of about 1,500 meters, they need suitable climatic conditions to grow, so they have not been domesticated and cultivated artificially. [0003] Tree head vegetable has high edible nutritional value and medicinal value of traditional Chinese medicine. It is rich in protein and mineral elements,...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 荣德福黄世杰李雪莲郑建君
Owner 中华人民共和国北仑出入境检验检疫局
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