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Method for preparing super-stable copper sulfide nano-cluster and application thereof

A copper sulfide and nano-cluster technology, applied in the field of bio-nano, can solve the problems of complex synthesis conditions, development limitations, and no simple synthesis, and achieve the effects of simple synthesis method, low preparation cost, and simple method.

Inactive Publication Date: 2015-05-13
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, copper sulfide has a variety of phases, and the synthesis methods are various, and the synthesis conditions are complicated, such as high temperature, inert gas protection, etc., so its development is limited.
In particular, the synthesis of copper sulfide nanoclusters has not been easily synthesized at room temperature.

Method used

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  • Method for preparing super-stable copper sulfide nano-cluster and application thereof
  • Method for preparing super-stable copper sulfide nano-cluster and application thereof
  • Method for preparing super-stable copper sulfide nano-cluster and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The preparation method of ultra-stable copper sulfide nanoclusters is as follows (flow process see figure 1 ):

[0033] 0.10mmol CuCl 2 2H 2 O and 0.08mmol sodium citrate dihydrate were dissolved in 100mL ultrapure water; after stirring at room temperature for 5min, add 0.1mmol Na 2 S·9H 2 O (the molar ratio of copper to sulfur is 1:1), the solution changes from light blue to brownish yellow, indicating that there is an intermediate state of copper sulfide nanoclusters. Add 0.1 mmol MUH molecules to the above solution, and stir overnight at room temperature. Then use a dialysis bag with a molecular weight cutoff (MWCO) of 3.5K to dialyze in ultrapure water for 3 days to remove excess MUH molecules to obtain pure copper sulfide nanoclusters. Its particle size is about 5nm (see figure 2 ), and has absorption in the near-infrared region (see image 3 ).

[0034] Anti-cancer effect evaluation: MDA-MB-231 breast cancer cells were selected and incubated with copper s...

Embodiment 2

[0039] The preparation method of ultra-stable copper sulfide nanoclusters is as follows:

[0040] 0.12mmol CuCl 2 2H 2 O and 1.00mmol sodium citrate dihydrate were dissolved in 100mL ultrapure water; after stirring at room temperature for 5min, directly add 0.1mmol Na 2 S·9H 2 O and 1 mmol MUH, stirred overnight at room temperature. Then use a dialysis bag with a molecular weight cutoff (MWCO) of 3.5K to dialyze in ultrapure water for 3 days to remove excess MUH molecules to obtain pure copper sulfide nanoclusters.

Embodiment 3

[0042] The preparation method of ultra-stable copper sulfide nanoclusters is as follows:

[0043] 0.08mmol CuCl 2 2H 2 O and 0.10 mmol sodium citrate dihydrate were dissolved in 100 mL ultrapure water; after stirring at room temperature for 5 min, 0.1 mmol Na 2 S·9H 2 O and 0.02 mmol MUH, stirred overnight at room temperature. Then use a dialysis bag with a molecular weight cutoff (MWCO) of 3.5K to dialyze in ultrapure water for 3 days to remove excess MUH molecules to obtain pure copper sulfide nanoclusters.

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Abstract

The invention provides a method for preparing a super-stable copper sulfide nano-cluster. The method comprises the following steps: mixing a soluble copper salt solution and a protective agent at room temperature, mixing with a sulfurizing salt solution, thereby obtaining a copper sulfide nano-cluster reaction intermediate state solution; adding a trapping agent into the copper sulfide nano-cluster reaction intermediate state solution, stirring and reacting at room temperature, thereby obtaining a stable copper sulfide nano-cluster solution; dialyzing the stable copper sulfide nano-cluster solution in a dialysis bag, thereby obtaining the pure copper sulfide nano-cluster solution; or the method comprises the following steps: mixing a soluble copper salt solution, a protective agent, a sulfurizing salt solution and a trapping agent, stirring and reacting at room temperature, thereby obtaining MUH-stable copper sulfide nano-cluster solution by virtue of a one-pot process; and dialyzing the MUH-stable copper sulfide nano-cluster solution in a dialysis bag, thereby obtaining the pure copper sulfide nano-cluster solution. The copper sulfide nano-cluster prepared by the method has high stability, high dispersity and high cell and bacteria toxicity and can be used for developing anti-tumor drugs and antibacterial products.

Description

technical field [0001] The invention belongs to the field of bio-nano technology, and in particular relates to a normal-temperature preparation method of ultra-stable copper sulfide nano-clusters, and also relates to the application of the nano-clusters in preparing anticancer and antibacterial drugs. Background technique [0002] Bacterial infection and cancer are major diseases that threaten human health. Drug resistance caused by the application of traditional antibiotics and anticancer drugs is becoming more and more serious. With the development of nanotechnology, a large number of new materials have been developed for antibacterial and anticancer applications, such as functional polymers, graphene and metal nanoparticles (such as silver nanoparticles are widely used as antibacterial and anticancer products). Among them, copper-based nanoparticles have attracted more and more attention due to their low cost compared with gold and silver materials, and the research and ...

Claims

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Application Information

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IPC IPC(8): C01G3/12B82Y30/00B82Y40/00A61K33/34A61P31/04A61P35/00
CPCA61K33/34C01G3/12C01P2002/84C01P2004/04C01P2004/51C01P2004/64
Inventor 吴富根王宏银华先武
Owner SOUTHEAST UNIV
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