Sesame photoperiod sensitive genic male sterile line and hybrid seed production method thereof
A hybrid seed production, light-sensitive technology, applied in the fields of botanical equipment and methods, plant genetic improvement, angiosperms/flowering plants, etc., can solve the limitations of large-scale promotion of sesame hybrids, incomplete removal of fertile plants, and fertility. Identify problems such as heavy workload, and achieve the effect of improving low utilization efficiency, increasing farmers' income and good seed production effect
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Embodiment 1
[0022] 1. Sesame light-sensitive male sterile line
[0023] The sesame light-sensitive genic male sterile line Sipsms3041 was planted in the sesame experimental base of Henan Academy of Agricultural Sciences (Zhengzhou). Field observation confirmed that the stamens of the sterile line were green and had no powder or little powder. Take the flowers to be opened, Alexander staining observation shows that most of the pollen grains are abortive type, the ratio is more than 99%, see figure 1 -Left picture; under self-crossing conditions, only 2-3 capsules on a single plant are enlarged, generally without seeds, see figure 2 - Left image. In Hainan area, the male sterile line showed fine powder, with yellow-green stamens and a small amount of pollen. The staining observation of pollen grains shows that there are two types of pollen grains: normal type and aborted type, and the proportion of normal pollen is about 33%, see figure 1 - In the middle picture, the number of capsules...
Embodiment 2
[0028] In December 2012, Sipsms3041 was planted in the Sanya Sesame Experimental Base in winter under the condition of net isolation, and the sterile line was propagated at a density of 15,000 plants / mu. Under the condition of short light, the fertility of the light-sensitive male sterile line can be recovered to a certain extent, and it can bear fruit. After the seeds matured, the inbreds were harvested.
[0029]In June 2013, under the condition of natural isolation without planting other sesame varieties within 2 kilometers around, the light-sensitive male sterile line Sipsms3041 was used as the female parent, and the sesame variety "Danbag" was used as the male parent at a row ratio of 2:1 Planted at intervals in Zhengzhou Sesame Seed Production Base, see image 3 . Using natural peaking to achieve insect pollination and obtain hybrid F 1 . When the seeds are fully mature, the F on the sterile line plants are harvested. 1 Seeds are hybrids. In the summer of 2014, a sm...
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