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Enzymatic method for preparing bone gelatin

An enzymatic preparation and bone gelatin technology, applied in the direction of fermentation, can solve the problems of gelatin quality influence, enzyme inactivation, etc., and achieve the effect of shortening the period of degreasing and gelatinization, high degreasing rate, and shortening the production cycle.

Inactive Publication Date: 2015-05-06
HUNAN ER KANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method uses high temperature to inactivate the enzyme. High temperature can not only inactivate the enzyme, but also have a certain impact on the quality of gelatin.

Method used

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  • Enzymatic method for preparing bone gelatin

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Effect test

Embodiment 1

[0037] The present embodiment adopts the pork bone of 1 kg, prepares bone gelatin by the method of the present invention, comprises the following steps;

[0038] (1) Degreasing: Put the bone particles into a pulverizer for crushing, the particle size is ≤2 mm, then add acetone to the bone meal for degreasing, the volume of acetone is 5 L, soak at room temperature for 4 h;

[0039] (2) Add water to make slurry: add 2 kg of deionized water to the defatted bone meal, stir to make a bone meal slurry;

[0040] (3) Homogenizer pulverization: add the bone meal slurry into the homogenizer to make it finer, with a particle size of 10 μm;

[0041](4) Acidification with citric acid: add citric acid with a concentration of 0.05mol / L to the defatted bone powder, the weight ratio of citric acid to bone powder is 0.1:1, and acidify at room temperature for 4 hours to obtain a collagen solution;

[0042] (5) Hydrogen peroxide decolorization: Add hydrogen peroxide with a concentration of 10% t...

Embodiment 2

[0050] The present embodiment adopts the bovine bone of 1 kg, prepares bone gelatin by the method of the present invention, comprises the following steps;

[0051] (1) Degreasing: Put the bone particles into a pulverizer for crushing, then add 8 L of acetone to the bone powder for degreasing, and soak for 8 hours at room temperature;

[0052] (2) Add water to make slurry: add deionized water to the degreased bone meal, the weight of deionized water is 3 times that of the bone meal, stir to make a bone meal slurry;

[0053] (3) Homogenizer pulverization: add the bone meal slurry into the homogenizer to make it finer, with a particle size of 20 μm;

[0054] (4) Acidification with citric acid: add citric acid with a concentration of 0.1mol / L to the defatted bone powder for acidification, and the weight ratio of citric acid to bone powder is 0.5:1 to obtain a collagen solution;

[0055] (5) Hydrogen peroxide decolorization: Add hydrogen peroxide with a concentration of 12% to the...

Embodiment 3

[0060] The present embodiment adopts the sheep bone of 1 kg, prepares bone gelatin by the method of the present invention, comprises the following steps;

[0061] (1) Degreasing: Put the bone particles into a pulverizer for crushing, then add 10L acetone to the bone meal for degreasing, and soak at room temperature for 12 hours;

[0062] (2) Add water to make slurry: add deionized water to the degreased bone meal, the weight of the deionized water is 4 times that of the bone meal, stir to make a bone meal slurry;

[0063] (3) Acidification with citric acid: add citric acid with a concentration of 0.3 mol / L to the defatted bone powder for acidification, and the weight ratio of citric acid to bone powder is 0.8:1 to obtain a collagen solution;

[0064] (4) Homogenizer pulverization: add the bone meal slurry into the homogenizer to make it finer, with a particle size of 30 μm;

[0065] (5) Hydrogen peroxide decolorization: Add hydrogen peroxide with a concentration of 15% to the...

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Abstract

The invention belongs to the field of preparation of gelatin and particularly relates to an enzymatic method for preparing bone gelatin. The enzymatic method comprises the following steps: preparing bone particles into degreased bone meal, adding water, stirring and pulping, acidifying with citric acid, adding hydrogen peroxide, decolorizing, washing with water, adding a biological combined enzyme, extracting glue, centrifuging, filtering, carrying out ion exchange, concentrating and drying. The enzymatic method has the advantages that (1) the bone particles are degreased with acetone so that the degreasing is more complete and the amino acid structure of the collagen can be retained; (2) the acidification is performed with citric acid so that the steps of pickling and liming are omitted, the discharge of wastewater and waste residues is significantly reduced, the production period is shortened and the cost is reduced; and (3) the biological combined enzyme can give fully play, the extraction of collagen is facilitated and the quality and yield of high-quality gelatin are significantly improved.

Description

technical field [0001] The invention relates to a method for preparing gelatin, in particular to a method for enzymatically preparing bone gelatin. Background technique [0002] Gelatin is a protein obtained by degrading and extracting animal skin and bone. It is mainly composed of 18 kinds of amino acid polypeptide molecular mixtures with the same composition and a wide molecular weight distribution. The molecular weight of gelatin is generally tens of thousands to hundreds of thousands. There are few amino acids containing sulfhydryl, but the content of four amino acids, glycine, alanine, proline and hydroxyproline, is very high, accounting for about 67% of the total amino acid content. Because gelatin is both acidic and alkaline, it is an amphoteric substance, and gelatin micelles are charged, and under the action of an electric field, it will move to one of the two poles. The molecular structure of gelatin contains a large number of hydroxyl groups, as well as many carb...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 帅放文王向峰章家伟
Owner HUNAN ER KANG PHARMA
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